##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
Acc65I AccI AccII AclI AflII
AgeI AocI Aor51HI AscI AseI
AsnI Asp718I AspI AviII BbvCI
BcgI BclI BfrI BglI BsiEI
BsiWI BsmI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CpoI DraI DraIII DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MfeI MluI
MunI MvnI NdeI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI SspI StuI SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 1991 GACGTC GACGT^C
AccIII 1675 TCCGGA T^CCGGA
AhdI 1517 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 956 GTGCAC G^TGCAC
ApaI 2124 GGGCCC GGGCC^C
ApaLI 956 GTGCAC G^TGCAC
Asp700I 1591 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1517 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1363 CCTAGG C^CTAGG
BalI 323 TGGCCA TGG^CCA
BamHI 315 GGATCC G^GATCC
BbeI 146 GGCGCC GGCGC^C
BbrPI 1391 CACGTG CAC^GTG
BciVI 15 GTATCC GTATCC(6/5)
BglII 1938 AGATCT A^GATCT
BlnI 1363 CCTAGG C^CTAGG
BsaAI 1391 CACGTG [CT]AC^GT[GA]
BsaBI 970 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 371 GAGACC GGTCTC(1/5)
BseAI 1675 TCCGGA T^CCGGA
Bsp1407I 1577 TGTACA T^GTACA
BspEI 1675 TCCGGA T^CCGGA
BspHI 363 TCATGA T^CATGA
BsrFI 577 GCCGGC [GA]^CCGG[CT]
BsrGI 1577 TGTACA T^GTACA
BssSI 353 CACGAG CACGAG(-5/-1)
BstXI 1705 CCATCTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 577 GCCGGC [GA]^CCGG[CT]
Eam1105I 1517 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1735 GAATTC G^AATTC
HaeII 146 GGCGCC [GA]GCGC^[CT]
KasI 146 GGCGCC G^GCGCC
MamI 970 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 323 TGGCCA TGG^CCA
MroI 1675 TCCGGA T^CCGGA
MscI 323 TGGCCA TGG^CCA
NaeI 577 GCCGGC GCC^GGC
NarI 146 GGCGCC GG^CGCC
NgoMIV 577 GCCGGC G^CCGGC
NheI 556 GCTAGC G^CTAGC
PmaCI 1391 CACGTG CAC^GTG
PmlI 1391 CACGTG CAC^GTG
PpuMI 1185 AGGACCT [GA]G^G[AT]CC[CT]
PstI 272 CTGCAG CTGCA^G
RcaI 363 TCATGA T^CATGA
SapI 1696 GAAGAGC GCTCTTC(1/4)
SbfI 271 CCTGCAGG CCTGCA^GG
ScaI 1825 AGTACT AGT^ACT
SfoI 146 GGCGCC GGC^GCC
SmlI 1044 CTCAAG C^T[CT][GA]AG
Sse8387I 271 CCTGCAGG CCTGCA^GG
SspBI 1577 TGTACA T^GTACA
XmnI 1591 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 159 ACATGT A^C[GA][CT]GT
945 ACATGT
AvaI 1379 CCCGGG C^[CT]CG[GA]G
2097 CCCGGG
BanI 146 GGCGCC G^G[CT][GA]CC
1913 GGCACC
BlpI 263 GCTAAGC GC^T[ACGT]AGC
1440 GCTCAGC
Bpu1102I 263 GCTAAGC GC^T[ACGT]AGC
1440 GCTCAGC
BsaWI 94 TCCGGT [AT]^CCGG[AT]
1675 TCCGGA
BsmBI 109 CGTCTC CGTCTC(1/5)
471 GAGACG
BsoBI 1379 CCCGGG C^[CT]CG[GA]G
2097 CCCGGG
BspDI 1277 ATCGAT AT^CGAT
1551 ATCGAT
BspLU11I 159 ACATGT A^CATGT
945 ACATGT
BsrBI 236 CCGCTC CCGCTC(-3/-3)
2042 GAGCGG
BsrDI 1774 CATTGC GCAATG(2/0)
1882 GCAATG
CelII 263 GCTAAGC GC^T[ACGT]AGC
1440 GCTCAGC
ClaI 1277 ATCGAT AT^CGAT
1551 ATCGAT
EaeI 323 TGGCCA [CT]^GGCC[GA]
1714 TGGCCG
HgaI 473 GACGC GACGC(5/10)
108 GCGTC
MseI 713 TTAA T^TAA
1986 TTAA
PflMI 253 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
306 CCATGGAATGG
PvuII 1808 CAGCTG CAG^CTG
1975 CAGCTG
SfcI 272 CTGCAG C^T[GA][CT]AG
1630 CTATAG
SmaI 1379 CCCGGG CCC^GGG
2097 CCCGGG
Tru9I 713 TTAA T^TAA
1986 TTAA
Van91I 253 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
306 CCATGGAATGG
XmaCI 1379 CCCGGG C^CCGGG
2097 CCCGGG
XmaI 1379 CCCGGG C^CCGGG
2097 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII