################################## # NO CUT ENZYMES ( 87 enzymes) # ################################## Acc65I AccI AccII AclI AflII AgeI AocI Aor51HI AscI AseI AsnI Asp718I AspI AviII BbvCI BcgI BclI BfrI BglI BsiEI BsiWI BsmI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MfeI MluI MunI MvnI NdeI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI SspI StuI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AatII 1991 GACGTC GACGT^C AccIII 1675 TCCGGA T^CCGGA AhdI 1517 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 956 GTGCAC G^TGCAC ApaI 2124 GGGCCC GGGCC^C ApaLI 956 GTGCAC G^TGCAC Asp700I 1591 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1517 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 1363 CCTAGG C^CTAGG BalI 323 TGGCCA TGG^CCA BamHI 315 GGATCC G^GATCC BbeI 146 GGCGCC GGCGC^C BbrPI 1391 CACGTG CAC^GTG BciVI 15 GTATCC GTATCC(6/5) BglII 1938 AGATCT A^GATCT BlnI 1363 CCTAGG C^CTAGG BsaAI 1391 CACGTG [CT]AC^GT[GA] BsaBI 970 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 371 GAGACC GGTCTC(1/5) BseAI 1675 TCCGGA T^CCGGA Bsp1407I 1577 TGTACA T^GTACA BspEI 1675 TCCGGA T^CCGGA BspHI 363 TCATGA T^CATGA BsrFI 577 GCCGGC [GA]^CCGG[CT] BsrGI 1577 TGTACA T^GTACA BssSI 353 CACGAG CACGAG(-5/-1) BstXI 1705 CCATCTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 577 GCCGGC [GA]^CCGG[CT] Eam1105I 1517 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 1735 GAATTC G^AATTC HaeII 146 GGCGCC [GA]GCGC^[CT] KasI 146 GGCGCC G^GCGCC MamI 970 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 323 TGGCCA TGG^CCA MroI 1675 TCCGGA T^CCGGA MscI 323 TGGCCA TGG^CCA NaeI 577 GCCGGC GCC^GGC NarI 146 GGCGCC GG^CGCC NgoMIV 577 GCCGGC G^CCGGC NheI 556 GCTAGC G^CTAGC PmaCI 1391 CACGTG CAC^GTG PmlI 1391 CACGTG CAC^GTG PpuMI 1185 AGGACCT [GA]G^G[AT]CC[CT] PstI 272 CTGCAG CTGCA^G RcaI 363 TCATGA T^CATGA SapI 1696 GAAGAGC GCTCTTC(1/4) SbfI 271 CCTGCAGG CCTGCA^GG ScaI 1825 AGTACT AGT^ACT SfoI 146 GGCGCC GGC^GCC SmlI 1044 CTCAAG C^T[CT][GA]AG Sse8387I 271 CCTGCAGG CCTGCA^GG SspBI 1577 TGTACA T^GTACA XmnI 1591 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AflIII 159 ACATGT A^C[GA][CT]GT 945 ACATGT AvaI 1379 CCCGGG C^[CT]CG[GA]G 2097 CCCGGG BanI 146 GGCGCC G^G[CT][GA]CC 1913 GGCACC BlpI 263 GCTAAGC GC^T[ACGT]AGC 1440 GCTCAGC Bpu1102I 263 GCTAAGC GC^T[ACGT]AGC 1440 GCTCAGC BsaWI 94 TCCGGT [AT]^CCGG[AT] 1675 TCCGGA BsmBI 109 CGTCTC CGTCTC(1/5) 471 GAGACG BsoBI 1379 CCCGGG C^[CT]CG[GA]G 2097 CCCGGG BspDI 1277 ATCGAT AT^CGAT 1551 ATCGAT BspLU11I 159 ACATGT A^CATGT 945 ACATGT BsrBI 236 CCGCTC CCGCTC(-3/-3) 2042 GAGCGG BsrDI 1774 CATTGC GCAATG(2/0) 1882 GCAATG CelII 263 GCTAAGC GC^T[ACGT]AGC 1440 GCTCAGC ClaI 1277 ATCGAT AT^CGAT 1551 ATCGAT EaeI 323 TGGCCA [CT]^GGCC[GA] 1714 TGGCCG HgaI 473 GACGC GACGC(5/10) 108 GCGTC MseI 713 TTAA T^TAA 1986 TTAA PflMI 253 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 306 CCATGGAATGG PvuII 1808 CAGCTG CAG^CTG 1975 CAGCTG SfcI 272 CTGCAG C^T[GA][CT]AG 1630 CTATAG SmaI 1379 CCCGGG CCC^GGG 2097 CCCGGG Tru9I 713 TTAA T^TAA 1986 TTAA Van91I 253 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 306 CCATGGAATGG XmaCI 1379 CCCGGG C^CCGGG 2097 CCCGGG XmaI 1379 CCCGGG C^CCGGG 2097 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 93 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI RsaI SacI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII