##################################
# NO CUT ENZYMES (   87 enzymes) #
##################################

Acc65I      AccI        AccII       AclI        AflII       
AgeI        AocI        Aor51HI     AscI        AseI        
AsnI        Asp718I     AspI        AviII       BbvCI       
BcgI        BclI        BfrI        BglI        BsiEI       
BsiWI       BsmI        BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstZ17I     
Bsu36I      CpoI        DraI        DraIII      DrdI        
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRV       EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KpnI        KspI        MfeI        MluI        
MunI        MvnI        NdeI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SexAI       
SfiI        SfuI        SgrAI       SnaBI       SpeI        
SphI        SspI        StuI        SwaI        Tth111I     
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AatII        1991  GACGTC               GACGT^C
AccIII       1675  TCCGGA               T^CCGGA
AhdI         1517  GACATCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        956  GTGCAC               G^TGCAC
ApaI         2124  GGGCCC               GGGCC^C
ApaLI         956  GTGCAC               G^TGCAC
Asp700I      1591  GAACCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1517  GACATCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII        1363  CCTAGG               C^CTAGG
BalI          323  TGGCCA               TGG^CCA
BamHI         315  GGATCC               G^GATCC
BbeI          146  GGCGCC               GGCGC^C
BbrPI        1391  CACGTG               CAC^GTG
BciVI          15  GTATCC               GTATCC(6/5)
BglII        1938  AGATCT               A^GATCT
BlnI         1363  CCTAGG               C^CTAGG
BsaAI        1391  CACGTG               [CT]AC^GT[GA]
BsaBI         970  GATGCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          371  GAGACC               GGTCTC(1/5)
BseAI        1675  TCCGGA               T^CCGGA
Bsp1407I     1577  TGTACA               T^GTACA
BspEI        1675  TCCGGA               T^CCGGA
BspHI         363  TCATGA               T^CATGA
BsrFI         577  GCCGGC               [GA]^CCGG[CT]
BsrGI        1577  TGTACA               T^GTACA
BssSI         353  CACGAG               CACGAG(-5/-1)
BstXI        1705  CCATCTCTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        577  GCCGGC               [GA]^CCGG[CT]
Eam1105I     1517  GACATCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI        1735  GAATTC               G^AATTC
HaeII         146  GGCGCC               [GA]GCGC^[CT]
KasI          146  GGCGCC               G^GCGCC
MamI          970  GATGCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         323  TGGCCA               TGG^CCA
MroI         1675  TCCGGA               T^CCGGA
MscI          323  TGGCCA               TGG^CCA
NaeI          577  GCCGGC               GCC^GGC
NarI          146  GGCGCC               GG^CGCC
NgoMIV        577  GCCGGC               G^CCGGC
NheI          556  GCTAGC               G^CTAGC
PmaCI        1391  CACGTG               CAC^GTG
PmlI         1391  CACGTG               CAC^GTG
PpuMI        1185  AGGACCT              [GA]G^G[AT]CC[CT]
PstI          272  CTGCAG               CTGCA^G
RcaI          363  TCATGA               T^CATGA
SapI         1696  GAAGAGC              GCTCTTC(1/4)
SbfI          271  CCTGCAGG             CCTGCA^GG
ScaI         1825  AGTACT               AGT^ACT
SfoI          146  GGCGCC               GGC^GCC
SmlI         1044  CTCAAG               C^T[CT][GA]AG
Sse8387I      271  CCTGCAGG             CCTGCA^GG
SspBI        1577  TGTACA               T^GTACA
XmnI         1591  GAACCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AflIII        159  ACATGT               A^C[GA][CT]GT
              945  ACATGT              
AvaI         1379  CCCGGG               C^[CT]CG[GA]G
             2097  CCCGGG              
BanI          146  GGCGCC               G^G[CT][GA]CC
             1913  GGCACC              
BlpI          263  GCTAAGC              GC^T[ACGT]AGC
             1440  GCTCAGC             
Bpu1102I      263  GCTAAGC              GC^T[ACGT]AGC
             1440  GCTCAGC             
BsaWI          94  TCCGGT               [AT]^CCGG[AT]
             1675  TCCGGA              
BsmBI         109  CGTCTC               CGTCTC(1/5)
              471  GAGACG              
BsoBI        1379  CCCGGG               C^[CT]CG[GA]G
             2097  CCCGGG              
BspDI        1277  ATCGAT               AT^CGAT
             1551  ATCGAT              
BspLU11I      159  ACATGT               A^CATGT
              945  ACATGT              
BsrBI         236  CCGCTC               CCGCTC(-3/-3)
             2042  GAGCGG              
BsrDI        1774  CATTGC               GCAATG(2/0)
             1882  GCAATG              
CelII         263  GCTAAGC              GC^T[ACGT]AGC
             1440  GCTCAGC             
ClaI         1277  ATCGAT               AT^CGAT
             1551  ATCGAT              
EaeI          323  TGGCCA               [CT]^GGCC[GA]
             1714  TGGCCG              
HgaI          473  GACGC                GACGC(5/10)
              108  GCGTC               
MseI          713  TTAA                 T^TAA
             1986  TTAA                
PflMI         253  CCACAAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              306  CCATGGAATGG         
PvuII        1808  CAGCTG               CAG^CTG
             1975  CAGCTG              
SfcI          272  CTGCAG               C^T[GA][CT]AG
             1630  CTATAG              
SmaI         1379  CCCGGG               CCC^GGG
             2097  CCCGGG              
Tru9I         713  TTAA                 T^TAA
             1986  TTAA                
Van91I        253  CCACAAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              306  CCATGGAATGG         
XmaCI        1379  CCCGGG               C^CCGGG
             2097  CCCGGG              
XmaI         1379  CCCGGG               C^CCGGG
             2097  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   93 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApoI        AspHI       AvaII       
BanII       BbsI        BbvI        BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaHI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EarI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PleI        RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       StyI        TaqI        
TfiI        TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XcmI        XhoII