##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
Acc65I AccI AclI AflII AflIII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BbrPI BciVI BclI BfrI
BglI BglII BlnI BsaAI BsiWI
Bsp1407I BspHI BspLU11I BsrBI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI FbaI FseI FspI
HindIII HpaI KpnI KspI MfeI
MluI MunI NcoI NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I RcaI RsrII SacII SalI
SapI SbfI ScaI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AatII 562 GACGTC GACGT^C
AccII 1410 CGCG CG^CG
AccIII 1372 TCCGGA T^CCGGA
AcsI 230 AAATTC [GA]^AATT[CT]
Alw44I 702 GTGCAC G^TGCAC
AlwNI 1318 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1051 CCTCAGG CC^T[ACGT]AGG
ApaI 1571 GGGCCC GGGCC^C
ApaLI 702 GTGCAC G^TGCAC
ApoI 230 AAATTC [GA]^AATT[CT]
Asp700I 326 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 598 CCCGAG C^[CT]CG[GA]G
BamHI 488 GGATCC G^GATCC
BcgI 251 CGATCGGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 1376 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1372 TCCGGA [AT]^CCGG[AT]
BseAI 1372 TCCGGA T^CCGGA
BsmBI 1157 CGTCTC CGTCTC(1/5)
BsoBI 598 CCCGAG C^[CT]CG[GA]G
BspDI 249 ATCGAT AT^CGAT
BspEI 1372 TCCGGA T^CCGGA
BspMI 359 GCAGGT ACCTGC(4/8)
BsrDI 1472 CATTGC GCAATG(2/0)
BsrFI 815 GCCGGC [GA]^CCGG[CT]
BstAPI 1095 GCACAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1410 CGCG CG^CG
Bsu36I 1051 CCTCAGG CC^T[ACGT]AGG
Cfr10I 815 GCCGGC [GA]^CCGG[CT]
ClaI 249 ATCGAT AT^CGAT
EarI 44 GAAGAG CTCTTC(1/4)
Eco81I 1051 CCTCAGG CC^T[ACGT]AGG
EcoNI 904 CCTCGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 312 GATATC GAT^ATC
EcoT14I 869 CCAAGG C^C[AT][AT]GG
EcoT22I 1284 ATGCAT ATGCA^T
HgaI 1468 GACGC GACGC(5/10)
HincII 10 GTCAAC GT[CT]^[GA]AC
HindII 10 GTCAAC GT[CT]^[GA]AC
Ksp632I 44 GAAGAG CTCTTC(1/4)
MamI 1376 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1372 TCCGGA T^CCGGA
MseI 199 TTAA T^TAA
MvnI 1410 CGCG CG^CG
NaeI 815 GCCGGC GCC^GGC
NdeI 1577 CATATG CA^TATG
NgoMIV 815 GCCGGC G^CCGGC
NsiI 1284 ATGCAT ATGCA^T
NspI 170 ACATGC [GA]CATG^[CT]
PshAI 892 GACCGTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 528 CTGCAG CTGCA^G
PvuI 251 CGATCG CGAT^CG
SacI 1595 GAGCTC GAGCT^C
SexAI 1244 ACCTGGT A^CC[AT]GGT
StuI 982 AGGCCT AGG^CCT
StyI 869 CCAAGG C^C[AT][AT]GG
Tru9I 199 TTAA T^TAA
XbaI 1464 TCTAGA T^CTAGA
XmnI 326 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
BalI 1204 TGGCCA TGG^CCA
1450 TGGCCA
BbsI 177 GAAGAC GAAGAC(2/6)
540 GAAGAC
BcnI 61 CCCGG CC^[GC]GG
74 CCGGG
BfaI 745 CTAG C^TAG
1465 CTAG
BpmI 1000 CTCCAG CTGGAG(16/14)
1119 CTCCAG
BpuAI 177 GAAGAC GAAGAC(2/6)
540 GAAGAC
BsaI 299 GGTCTC GGTCTC(1/5)
748 GGTCTC
BsiEI 251 CGATCG CG[GA][CT]^CG
891 CGACCG
BssSI 5 CACGAG CACGAG(-5/-1)
882 CACGAG
BstXI 100 CCAGTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1369 CCATCCGGATGG
BstYI 488 GGATCC [GA]^GATC[CT]
726 GGATCT
DsaI 286 CCACGG C^C[GA][CT]GG
707 CCACGG
EaeI 1204 TGGCCA [CT]^GGCC[GA]
1450 TGGCCA
FauI 425 CCCGC CCCGC(4/6)
1427 GCGGG
MaeI 745 CTAG C^TAG
1465 CTAG
MflI 488 GGATCC [GA]^GATC[CT]
726 GGATCT
MluNI 1204 TGGCCA TGG^CCA
1450 TGGCCA
MscI 1204 TGGCCA TGG^CCA
1450 TGGCCA
MslI 1370 CATCCGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1472 CATTGCTGTG
NciI 61 CCCGG CC^[GC]GG
74 CCGGG
PpuMI 975 GGGTCCC [GA]G^G[AT]CC[CT]
1226 AGGTCCC
TaqI 55 TCGA T^CGA
250 TCGA
Tsp509I 231 AATT ^AATT
1034 AATT
TthHB8I 55 TCGA T^CGA
250 TCGA
XhoII 488 GGATCC [GA]^GATC[CT]
726 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AspHI AvaII BanI BanII BbeI
BbvCI BbvI BlpI BmyI Bpu1102I
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI MaeII MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MwoI NarI NdeII NlaIII NlaIV
PflMI PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
TfiI TseI Tsp45I TspRI Van91I
XcmI