################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII AccIII AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspI AvaI AviII BamHI BanII BbeI BbrPI BbsI BclI BfrI BglII BlpI Bpu1102I BpuAI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrFI BsrGI BssHII Bst1107I BstAPI BstEII BstPI BstYI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI FbaI FseI FspI HaeII HhaI HinP1I HpaI KasI KspI MfeI MflI MluI MroI MunI NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SgrAI SmaI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### Acc65I 1688 GGTACC G^GTACC AccI 685 GTCTAC GT^[AC][GT]AC AccII 640 CGCG CG^CG AcyI 88 GACGCC G[GA]^CG[CT]C AocI 838 CCTCAGG CC^T[ACGT]AGG Asp700I 288 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1688 GGTACC G^GTACC AvaII 1412 GGTCC G^G[AT]CC BalI 1421 TGGCCA TGG^CCA BbvCI 505 CCTCAGC CCTCAGC(-5/-2) BcgI 178 CGAGTGGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 762 CCCGG CC^[GC]GG BglI 1499 GCCAGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 1710 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 88 GACGCC G[GA]^CG[CT]C BseRI 700 GAGGAG GAGGAG(10/8) BsmAI 1166 GTCTC GTCTC(1/5) BspHI 1760 TCATGA T^CATGA BsrDI 806 GCAATG GCAATG(2/0) BsrI 579 ACTGG ACTGG(1/-1) BssSI 1425 CACGAG CACGAG(-5/-1) BstBI 1389 TTCGAA TT^CGAA BstUI 640 CGCG CG^CG BstXI 1808 CCATCTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 838 CCTCAGG CC^T[ACGT]AGG DraIII 158 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1331 CCGTGG C^C[GA][CT]GG Eco81I 838 CCTCAGG CC^T[ACGT]AGG EcoRV 40 GATATC GAT^ATC HapII 763 CCGG C^CGG Hin1I 88 GACGCC G[GA]^CG[CT]C HincII 943 GTTGAC GT[CT]^[GA]AC HindII 943 GTTGAC GT[CT]^[GA]AC HindIII 706 AAGCTT A^AGCTT HpaII 763 CCGG C^CGG KpnI 1688 GGTACC GGTAC^C MamI 1710 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1421 TGGCCA TGG^CCA MscI 1421 TGGCCA TGG^CCA MspI 763 CCGG C^CGG MvnI 640 CGCG CG^CG NciI 762 CCCGG CC^[GC]GG NspI 1181 ACATGC [GA]CATG^[CT] NspV 1389 TTCGAA TT^CGAA PflMI 1528 CCAAGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1411 AGGTCCT [GA]G^G[AT]CC[CT] PshAI 678 GACTCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1760 TCATGA T^CATGA SexAI 733 ACCAGGT A^CC[AT]GGT SfuI 1389 TTCGAA TT^CGAA SmlI 1168 CTCAAG C^T[CT][GA]AG StuI 1506 AGGCCT AGG^CCT Van91I 1528 CCAAGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 541 TCTAGA T^CTAGA XcmI 716 CCAAAAACAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 288 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcsI 110 AAATTT [GA]^AATT[CT] 1224 GAATTT AlwNI 515 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG 1618 CAGAGCCTG ApoI 110 AAATTT [GA]^AATT[CT] 1224 GAATTT AspHI 418 GAGCAC G[AT]GC[AT]^C 644 GTGCTC AvrII 1404 CCTAGG C^CTAGG 1509 CCTAGG BciVI 758 GTATCC GTATCC(6/5) 921 GTATCC BlnI 1404 CCTAGG C^CTAGG 1509 CCTAGG BsiHKAI 418 GAGCAC G[AT]GC[AT]^C 644 GTGCTC BsmI 969 GAATGC GAATGC(1/-1) 1667 GAATGC DraII 996 AGGCCCC [GA]G^G[ACGT]CC[CT] 1411 AGGTCCT EaeI 764 CGGCCA [CT]^GGCC[GA] 1421 TGGCCA EarI 1295 CTCTTC CTCTTC(1/4) 1159 GAAGAG EcoO109I 996 AGGCCCC [GA]G^G[ACGT]CC[CT] 1411 AGGTCCT FauI 80 CCCGC CCCGC(4/6) 1340 CCCGC HgaI 88 GACGC GACGC(5/10) 1290 GCGTC HphI 582 GGTGA GGTGA(8/7) 1610 TCACC Ksp632I 1295 CTCTTC CTCTTC(1/4) 1159 GAAGAG MslI 1809 CATCTTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1853 CATCTTTGTG NdeI 1547 CATATG CA^TATG 1777 CATATG SnaBI 116 TACGTA TAC^GTA 456 TACGTA TaqI 1390 TCGA T^CGA 1606 TCGA Tsp45I 932 GTGAC ^GT[GC]AC 1262 GTCAC TthHB8I 1390 TCGA T^CGA 1606 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AciI AfaI AluI AlwI BanI BbvI BfaI BmyI BpmI BsaAI BsaJI BsiYI BslI BsmFI Bsp1286I BsrBI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HinfI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV NsiI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp509I TspRI