##################################
# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI AspEI
AspI AvaI AviII BamHI BanII
BbeI BbrPI BbsI BclI BfrI
BglII BlpI Bpu1102I BpuAI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrFI BsrGI BssHII
Bst1107I BstAPI BstEII BstPI BstYI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI FbaI FseI FspI HaeII
HhaI HinP1I HpaI KasI KspI
MfeI MflI MluI MroI MunI
NaeI NarI NcoI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SgrAI SmaI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 1688 GGTACC G^GTACC
AccI 685 GTCTAC GT^[AC][GT]AC
AccII 640 CGCG CG^CG
AcyI 88 GACGCC G[GA]^CG[CT]C
AocI 838 CCTCAGG CC^T[ACGT]AGG
Asp700I 288 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1688 GGTACC G^GTACC
AvaII 1412 GGTCC G^G[AT]CC
BalI 1421 TGGCCA TGG^CCA
BbvCI 505 CCTCAGC CCTCAGC(-5/-2)
BcgI 178 CGAGTGGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 762 CCCGG CC^[GC]GG
BglI 1499 GCCAGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1710 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 88 GACGCC G[GA]^CG[CT]C
BseRI 700 GAGGAG GAGGAG(10/8)
BsmAI 1166 GTCTC GTCTC(1/5)
BspHI 1760 TCATGA T^CATGA
BsrDI 806 GCAATG GCAATG(2/0)
BsrI 579 ACTGG ACTGG(1/-1)
BssSI 1425 CACGAG CACGAG(-5/-1)
BstBI 1389 TTCGAA TT^CGAA
BstUI 640 CGCG CG^CG
BstXI 1808 CCATCTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 838 CCTCAGG CC^T[ACGT]AGG
DraIII 158 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1331 CCGTGG C^C[GA][CT]GG
Eco81I 838 CCTCAGG CC^T[ACGT]AGG
EcoRV 40 GATATC GAT^ATC
HapII 763 CCGG C^CGG
Hin1I 88 GACGCC G[GA]^CG[CT]C
HincII 943 GTTGAC GT[CT]^[GA]AC
HindII 943 GTTGAC GT[CT]^[GA]AC
HindIII 706 AAGCTT A^AGCTT
HpaII 763 CCGG C^CGG
KpnI 1688 GGTACC GGTAC^C
MamI 1710 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1421 TGGCCA TGG^CCA
MscI 1421 TGGCCA TGG^CCA
MspI 763 CCGG C^CGG
MvnI 640 CGCG CG^CG
NciI 762 CCCGG CC^[GC]GG
NspI 1181 ACATGC [GA]CATG^[CT]
NspV 1389 TTCGAA TT^CGAA
PflMI 1528 CCAAGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1411 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 678 GACTCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1760 TCATGA T^CATGA
SexAI 733 ACCAGGT A^CC[AT]GGT
SfuI 1389 TTCGAA TT^CGAA
SmlI 1168 CTCAAG C^T[CT][GA]AG
StuI 1506 AGGCCT AGG^CCT
Van91I 1528 CCAAGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 541 TCTAGA T^CTAGA
XcmI 716 CCAAAAACAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 288 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 110 AAATTT [GA]^AATT[CT]
1224 GAATTT
AlwNI 515 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1618 CAGAGCCTG
ApoI 110 AAATTT [GA]^AATT[CT]
1224 GAATTT
AspHI 418 GAGCAC G[AT]GC[AT]^C
644 GTGCTC
AvrII 1404 CCTAGG C^CTAGG
1509 CCTAGG
BciVI 758 GTATCC GTATCC(6/5)
921 GTATCC
BlnI 1404 CCTAGG C^CTAGG
1509 CCTAGG
BsiHKAI 418 GAGCAC G[AT]GC[AT]^C
644 GTGCTC
BsmI 969 GAATGC GAATGC(1/-1)
1667 GAATGC
DraII 996 AGGCCCC [GA]G^G[ACGT]CC[CT]
1411 AGGTCCT
EaeI 764 CGGCCA [CT]^GGCC[GA]
1421 TGGCCA
EarI 1295 CTCTTC CTCTTC(1/4)
1159 GAAGAG
EcoO109I 996 AGGCCCC [GA]G^G[ACGT]CC[CT]
1411 AGGTCCT
FauI 80 CCCGC CCCGC(4/6)
1340 CCCGC
HgaI 88 GACGC GACGC(5/10)
1290 GCGTC
HphI 582 GGTGA GGTGA(8/7)
1610 TCACC
Ksp632I 1295 CTCTTC CTCTTC(1/4)
1159 GAAGAG
MslI 1809 CATCTTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1853 CATCTTTGTG
NdeI 1547 CATATG CA^TATG
1777 CATATG
SnaBI 116 TACGTA TAC^GTA
456 TACGTA
TaqI 1390 TCGA T^CGA
1606 TCGA
Tsp45I 932 GTGAC ^GT[GC]AC
1262 GTCAC
TthHB8I 1390 TCGA T^CGA
1606 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AlwI BanI
BbvI BfaI BmyI BpmI BsaAI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrBI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HinfI
ItaI MaeI MaeII MaeIII MboI
MboII MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NsiI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TfiI Tru9I TseI Tsp509I TspRI