##################################
# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AviII AvrII BamHI BbeI
BbrPI BcgI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstF5I BstPI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FokI FseI
FspI HaeII HgaI Hin1I HindIII
HpaI KasI KpnI KspI MamI
MfeI MflI MluI MroI MslI
MunI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SspBI StuI StyI SwaI
TaqI TthHB8I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccII 748 CGCG CG^CG
AseI 231 ATTAAT AT^TAAT
AsnI 231 ATTAAT AT^TAAT
AspI 526 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 543 CTCGGG C^[CT]CG[GA]G
AvaII 1200 GGACC G^G[AT]CC
BanI 1094 GGCACC G^G[CT][GA]CC
BanII 1123 GAGCCC G[GA]GC[CT]^C
BciVI 1559 GGATAC GTATCC(6/5)
BcnI 1126 CCCGG CC^[GC]GG
BseRI 437 CTCCTC GAGGAG(10/8)
BsgI 1429 CTGCAC GTGCAG(16/14)
BsoBI 543 CTCGGG C^[CT]CG[GA]G
BspHI 1111 TCATGA T^CATGA
BsrDI 1642 CATTGC GCAATG(2/0)
BsrFI 1436 ACCGGC [GA]^CCGG[CT]
BstAPI 372 GCAAGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 748 CGCG CG^CG
CfoI 749 GCGC GCG^C
Cfr10I 1436 ACCGGC [GA]^CCGG[CT]
Cfr13I 1200 GGACC G^G[ACGT]CC
DpnII 1268 GATC ^GATC
HhaI 749 GCGC GCG^C
HinP1I 749 GCGC G^CGC
HincII 1445 GTTGAC GT[CT]^[GA]AC
HindII 1445 GTTGAC GT[CT]^[GA]AC
MboI 1268 GATC ^GATC
MvnI 748 CGCG CG^CG
NciI 1126 CCCGG CC^[GC]GG
NdeI 927 CATATG CA^TATG
NdeII 1268 GATC ^GATC
NlaIII 1112 CATG CATG^
PflFI 526 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1191 CCAAAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 366 GAGTC GAGTC(4/5)
PshBI 231 ATTAAT AT^TAAT
RcaI 1111 TCATGA T^CATGA
Sau3AI 1268 GATC ^GATC
Sau96I 1200 GGACC G^G[ACGT]CC
SmlI 1144 CTCAAG C^T[CT][GA]AG
SpeI 1020 ACTAGT A^CTAGT
Tth111I 526 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1191 CCAAAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 1140 CCGC CCGC(-3/-1)
481 GCGG
AcsI 128 AAATTC [GA]^AATT[CT]
1750 GAATTT
ApoI 128 AAATTC [GA]^AATT[CT]
1750 GAATTT
AspHI 112 GAGCAC G[AT]GC[AT]^C
1033 GTGCTC
BalI 556 TGGCCA TGG^CCA
1223 TGGCCA
BbvCI 1 CCTCAGC CCTCAGC(-5/-2)
868 CCTCAGC
BfaI 361 CTAG C^TAG
1021 CTAG
BsaJI 804 CCAGGG C^C[ACGT][ACGT]GG
879 CCAGGG
BsiHKAI 112 GAGCAC G[AT]GC[AT]^C
1033 GTGCTC
Cac8I 215 GCCAGC GC[ACGT]^[ACGT]GC
324 GCAAGC
EaeI 556 TGGCCA [CT]^GGCC[GA]
1223 TGGCCA
EarI 494 CTCTTC CTCTTC(1/4)
1654 CTCTTC
HapII 1127 CCGG C^CGG
1437 CCGG
HpaII 1127 CCGG C^CGG
1437 CCGG
Ksp632I 494 CTCTTC CTCTTC(1/4)
1654 CTCTTC
MaeI 361 CTAG C^TAG
1021 CTAG
MaeII 387 ACGT A^CGT
1168 ACGT
MluNI 556 TGGCCA TGG^CCA
1223 TGGCCA
MscI 556 TGGCCA TGG^CCA
1223 TGGCCA
MspI 1127 CCGG C^CGG
1437 CCGG
PstI 1372 CTGCAG CTGCA^G
1621 CTGCAG
ScaI 89 AGTACT AGT^ACT
951 AGTACT
SspI 333 AATATT AAT^ATT
894 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AfaI AluI BbsI BbvI BmyI
BpmI BpuAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstNI DdeI DraI EcoRII Fnu4HI
HaeIII HinfI HphI ItaI MaeIII
MboII MnlI MseI MspA1I MvaI
MwoI NlaIV PvuII RsaI ScrFI
SfaNI SfcI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI