################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AviII AvrII BamHI BbeI BbrPI BcgI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HgaI Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MflI MluI MroI MslI MunI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI StuI StyI SwaI TaqI TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccII 748 CGCG CG^CG AseI 231 ATTAAT AT^TAAT AsnI 231 ATTAAT AT^TAAT AspI 526 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 543 CTCGGG C^[CT]CG[GA]G AvaII 1200 GGACC G^G[AT]CC BanI 1094 GGCACC G^G[CT][GA]CC BanII 1123 GAGCCC G[GA]GC[CT]^C BciVI 1559 GGATAC GTATCC(6/5) BcnI 1126 CCCGG CC^[GC]GG BseRI 437 CTCCTC GAGGAG(10/8) BsgI 1429 CTGCAC GTGCAG(16/14) BsoBI 543 CTCGGG C^[CT]CG[GA]G BspHI 1111 TCATGA T^CATGA BsrDI 1642 CATTGC GCAATG(2/0) BsrFI 1436 ACCGGC [GA]^CCGG[CT] BstAPI 372 GCAAGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 748 CGCG CG^CG CfoI 749 GCGC GCG^C Cfr10I 1436 ACCGGC [GA]^CCGG[CT] Cfr13I 1200 GGACC G^G[ACGT]CC DpnII 1268 GATC ^GATC HhaI 749 GCGC GCG^C HinP1I 749 GCGC G^CGC HincII 1445 GTTGAC GT[CT]^[GA]AC HindII 1445 GTTGAC GT[CT]^[GA]AC MboI 1268 GATC ^GATC MvnI 748 CGCG CG^CG NciI 1126 CCCGG CC^[GC]GG NdeI 927 CATATG CA^TATG NdeII 1268 GATC ^GATC NlaIII 1112 CATG CATG^ PflFI 526 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1191 CCAAAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 366 GAGTC GAGTC(4/5) PshBI 231 ATTAAT AT^TAAT RcaI 1111 TCATGA T^CATGA Sau3AI 1268 GATC ^GATC Sau96I 1200 GGACC G^G[ACGT]CC SmlI 1144 CTCAAG C^T[CT][GA]AG SpeI 1020 ACTAGT A^CTAGT Tth111I 526 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1191 CCAAAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AciI 1140 CCGC CCGC(-3/-1) 481 GCGG AcsI 128 AAATTC [GA]^AATT[CT] 1750 GAATTT ApoI 128 AAATTC [GA]^AATT[CT] 1750 GAATTT AspHI 112 GAGCAC G[AT]GC[AT]^C 1033 GTGCTC BalI 556 TGGCCA TGG^CCA 1223 TGGCCA BbvCI 1 CCTCAGC CCTCAGC(-5/-2) 868 CCTCAGC BfaI 361 CTAG C^TAG 1021 CTAG BsaJI 804 CCAGGG C^C[ACGT][ACGT]GG 879 CCAGGG BsiHKAI 112 GAGCAC G[AT]GC[AT]^C 1033 GTGCTC Cac8I 215 GCCAGC GC[ACGT]^[ACGT]GC 324 GCAAGC EaeI 556 TGGCCA [CT]^GGCC[GA] 1223 TGGCCA EarI 494 CTCTTC CTCTTC(1/4) 1654 CTCTTC HapII 1127 CCGG C^CGG 1437 CCGG HpaII 1127 CCGG C^CGG 1437 CCGG Ksp632I 494 CTCTTC CTCTTC(1/4) 1654 CTCTTC MaeI 361 CTAG C^TAG 1021 CTAG MaeII 387 ACGT A^CGT 1168 ACGT MluNI 556 TGGCCA TGG^CCA 1223 TGGCCA MscI 556 TGGCCA TGG^CCA 1223 TGGCCA MspI 1127 CCGG C^CGG 1437 CCGG PstI 1372 CTGCAG CTGCA^G 1621 CTGCAG ScaI 89 AGTACT AGT^ACT 951 AGTACT SspI 333 AATATT AAT^ATT 894 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AfaI AluI BbsI BbvI BmyI BpmI BpuAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstNI DdeI DraI EcoRII Fnu4HI HaeIII HinfI HphI ItaI MaeIII MboII MnlI MseI MspA1I MvaI MwoI NlaIV PvuII RsaI ScrFI SfaNI SfcI TfiI Tru9I TseI Tsp45I Tsp509I TspRI