################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BalI BbeI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HpaI KasI KpnI MamI MfeI MluI MluNI MroI MscI MslI MunI NarI NdeI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI SspI StuI SwaI Tsp509I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AfaI 174 GTAC GT^AC AflIII 1709 ACACGT A^C[GA][CT]GT Alw44I 1694 GTGCAC G^TGCAC AocI 231 CCTGAGG CC^T[ACGT]AGG ApaLI 1694 GTGCAC G^TGCAC AspI 1236 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 1710 CACGTG CAC^GTG BsmBI 27 GAGACG CGTCTC(1/5) BssHII 41 GCGCGC G^CGCGC BstAPI 836 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 231 CCTGAGG CC^T[ACGT]AGG EarI 906 GAAGAG CTCTTC(1/4) Eco81I 231 CCTGAGG CC^T[ACGT]AGG EcoT14I 532 CCATGG C^C[AT][AT]GG HindIII 347 AAGCTT A^AGCTT Ksp632I 906 GAAGAG CTCTTC(1/4) KspI 250 CCGCGG CCGC^GG MboII 906 GAAGA GAAGA(8/7) MseI 9 TTAA T^TAA NaeI 1294 GCCGGC GCC^GGC NcoI 532 CCATGG C^CATGG NgoMIV 1294 GCCGGC G^CCGGC NheI 752 GCTAGC G^CTAGC PflFI 1236 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1710 CACGTG CAC^GTG PmlI 1710 CACGTG CAC^GTG RsaI 174 GTAC GT^AC SacI 759 GAGCTC GAGCT^C SacII 250 CCGCGG CCGC^GG SapI 906 GAAGAGC GCTCTTC(1/4) SexAI 1237 ACCTGGT A^CC[AT]GGT SfiI 246 GGCCCCGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SpeI 143 ACTAGT A^CTAGT StyI 532 CCATGG C^C[AT][AT]GG TfiI 1684 GATTC G^A[AT]TC Tru9I 9 TTAA T^TAA Tsp45I 1276 GTGAC ^GT[GC]AC Tth111I 1236 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccII 42 CGCG CG^CG 251 CGCG ApaI 245 GGGCCC GGGCC^C 1375 GGGCCC AspHI 759 GAGCTC G[AT]GC[AT]^C 1694 GTGCAC BamHI 59 GGATCC G^GATCC 555 GGATCC BfaI 144 CTAG C^TAG 753 CTAG BglI 247 GCCCCGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 256 GCCTCAAAGGC BsaAI 1691 TACGTG [CT]AC^GT[GA] 1710 CACGTG BsiHKAI 759 GAGCTC G[AT]GC[AT]^C 1694 GTGCAC BsmAI 27 GAGAC GTCTC(1/5) 278 GTCTC BspMI 791 GCAGGT ACCTGC(4/8) 836 GCAGGT BsrFI 999 GCCGGT [GA]^CCGG[CT] 1294 GCCGGC BstF5I 529 CATCC GGATG(2/0) 964 GGATG BstUI 42 CGCG CG^CG 251 CGCG Cfr10I 999 GCCGGT [GA]^CCGG[CT] 1294 GCCGGC EaeI 150 CGGCCA [CT]^GGCC[GA] 925 TGGCCG FokI 529 CATCC GGATG(9/13) 964 GGATG HphI 1002 GGTGA GGTGA(8/7) 600 TCACC MaeI 144 CTAG C^TAG 753 CTAG MvnI 42 CGCG CG^CG 251 CGCG PpuMI 225 GGGACCC [GA]G^G[AT]CC[CT] 483 GGGACCT SmlI 1142 CTTGAG C^T[CT][GA]AG 1212 CTCAAG TaqI 1243 TCGA T^CGA 1666 TCGA TthHB8I 1243 TCGA T^CGA 1666 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AciI AluI AlwI AlwNI AvaII BanI BanII BbvI BcnI BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BsrBI BsrI BssKI BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeII MaeIII MboI MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI TseI TspRI Van91I XhoII