##################################
# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AvaI AviII
AvrII BalI BbeI BbsI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BpuAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HpaI KasI
KpnI MamI MfeI MluI MluNI
MroI MscI MslI MunI NarI
NdeI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp509I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AfaI 174 GTAC GT^AC
AflIII 1709 ACACGT A^C[GA][CT]GT
Alw44I 1694 GTGCAC G^TGCAC
AocI 231 CCTGAGG CC^T[ACGT]AGG
ApaLI 1694 GTGCAC G^TGCAC
AspI 1236 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1710 CACGTG CAC^GTG
BsmBI 27 GAGACG CGTCTC(1/5)
BssHII 41 GCGCGC G^CGCGC
BstAPI 836 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 231 CCTGAGG CC^T[ACGT]AGG
EarI 906 GAAGAG CTCTTC(1/4)
Eco81I 231 CCTGAGG CC^T[ACGT]AGG
EcoT14I 532 CCATGG C^C[AT][AT]GG
HindIII 347 AAGCTT A^AGCTT
Ksp632I 906 GAAGAG CTCTTC(1/4)
KspI 250 CCGCGG CCGC^GG
MboII 906 GAAGA GAAGA(8/7)
MseI 9 TTAA T^TAA
NaeI 1294 GCCGGC GCC^GGC
NcoI 532 CCATGG C^CATGG
NgoMIV 1294 GCCGGC G^CCGGC
NheI 752 GCTAGC G^CTAGC
PflFI 1236 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1710 CACGTG CAC^GTG
PmlI 1710 CACGTG CAC^GTG
RsaI 174 GTAC GT^AC
SacI 759 GAGCTC GAGCT^C
SacII 250 CCGCGG CCGC^GG
SapI 906 GAAGAGC GCTCTTC(1/4)
SexAI 1237 ACCTGGT A^CC[AT]GGT
SfiI 246 GGCCCCGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 143 ACTAGT A^CTAGT
StyI 532 CCATGG C^C[AT][AT]GG
TfiI 1684 GATTC G^A[AT]TC
Tru9I 9 TTAA T^TAA
Tsp45I 1276 GTGAC ^GT[GC]AC
Tth111I 1236 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 42 CGCG CG^CG
251 CGCG
ApaI 245 GGGCCC GGGCC^C
1375 GGGCCC
AspHI 759 GAGCTC G[AT]GC[AT]^C
1694 GTGCAC
BamHI 59 GGATCC G^GATCC
555 GGATCC
BfaI 144 CTAG C^TAG
753 CTAG
BglI 247 GCCCCGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
256 GCCTCAAAGGC
BsaAI 1691 TACGTG [CT]AC^GT[GA]
1710 CACGTG
BsiHKAI 759 GAGCTC G[AT]GC[AT]^C
1694 GTGCAC
BsmAI 27 GAGAC GTCTC(1/5)
278 GTCTC
BspMI 791 GCAGGT ACCTGC(4/8)
836 GCAGGT
BsrFI 999 GCCGGT [GA]^CCGG[CT]
1294 GCCGGC
BstF5I 529 CATCC GGATG(2/0)
964 GGATG
BstUI 42 CGCG CG^CG
251 CGCG
Cfr10I 999 GCCGGT [GA]^CCGG[CT]
1294 GCCGGC
EaeI 150 CGGCCA [CT]^GGCC[GA]
925 TGGCCG
FokI 529 CATCC GGATG(9/13)
964 GGATG
HphI 1002 GGTGA GGTGA(8/7)
600 TCACC
MaeI 144 CTAG C^TAG
753 CTAG
MvnI 42 CGCG CG^CG
251 CGCG
PpuMI 225 GGGACCC [GA]G^G[AT]CC[CT]
483 GGGACCT
SmlI 1142 CTTGAG C^T[CT][GA]AG
1212 CTCAAG
TaqI 1243 TCGA T^CGA
1666 TCGA
TthHB8I 1243 TCGA T^CGA
1666 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BanI BanII BbvI BcnI BlpI
BmyI BpmI Bpu1102I BsaJI BseRI
BsiYI BslI BsmFI Bsp1286I BsrBI
BsrI BssKI BstNI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EcoO109I EcoRII FauI
Fnu4HI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeII
MaeIII MboI MflI MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PstI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI TseI TspRI Van91I XhoII