##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AccI AclI AcsI AcyI
AflII AhdI Aor51HI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AvrII BamHI BbeI BclI BfrI
BglII BlnI BsaBI BsaHI BsaI
BsiWI BsmBI Bsp1407I BspDI BspLU11I
BsrBI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I ClaI
DraI Eam1105I Eco47III EcoO65I EcoRI
EcoRV FbaI FseI Hin1I HpaI
KasI KspI MamI MfeI MluI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PflFI PmeI PshBI Psp1406I
PvuI SacII SalI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI SspBI SspI StuI SwaI
TfiI Tsp509I Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 456 GGTACC G^GTACC
AccIII 187 TCCGGA T^CCGGA
AflIII 1899 ACACGT A^C[GA][CT]GT
AgeI 198 ACCGGT A^CCGGT
AocI 809 CCTGAGG CC^T[ACGT]AGG
ApaI 1211 GGGCCC GGGCC^C
Asp718I 456 GGTACC G^GTACC
AviII 1327 TGCGCA TGC^GCA
BbrPI 1825 CACGTG CAC^GTG
BcgI 1580 GCACATGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2022 GTATCC GTATCC(6/5)
BglI 984 GCCCAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseAI 187 TCCGGA T^CCGGA
BsiEI 75 CGGCCG CG[GA][CT]^CG
BsmI 900 GAATGC GAATGC(1/-1)
BspEI 187 TCCGGA T^CCGGA
BspHI 880 TCATGA T^CATGA
BsrDI 1804 CATTGC GCAATG(2/0)
BssSI 506 CACGAG CACGAG(-5/-1)
Bsu36I 809 CCTGAGG CC^T[ACGT]AGG
CpoI 195 CGGACCG CG^G[AT]CCG
DraIII 1310 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1626 GACTCTCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 75 CGGCCG C^GGCCG
EarI 618 CTCTTC CTCTTC(1/4)
EclXI 75 CGGCCG C^GGCCG
Eco52I 75 CGGCCG C^GGCCG
Eco81I 809 CCTGAGG CC^T[ACGT]AGG
EcoT22I 902 ATGCAT ATGCA^T
FspI 1327 TGCGCA TGC^GCA
HincII 273 GTCAAC GT[CT]^[GA]AC
HindII 273 GTCAAC GT[CT]^[GA]AC
HindIII 1248 AAGCTT A^AGCTT
KpnI 456 GGTACC GGTAC^C
Ksp632I 618 CTCTTC CTCTTC(1/4)
MroI 187 TCCGGA T^CCGGA
MseI 697 TTAA T^TAA
NsiI 902 ATGCAT ATGCA^T
PflMI 1517 CCATCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 198 ACCGGT A^CCGGT
PmaCI 1825 CACGTG CAC^GTG
PmlI 1825 CACGTG CAC^GTG
RcaI 880 TCATGA T^CATGA
RsrII 195 CGGACCG CG^G[AT]CCG
SacI 183 GAGCTC GAGCT^C
SapI 617 GCTCTTC GCTCTTC(1/4)
SbfI 1790 CCTGCAGG CCTGCA^GG
ScaI 1387 AGTACT AGT^ACT
SmaI 609 CCCGGG CCC^GGG
Sse8387I 1790 CCTGCAGG CCTGCA^GG
Tru9I 697 TTAA T^TAA
Van91I 1517 CCATCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 609 CCCGGG C^CCGGG
XmaI 609 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Alw44I 1011 GTGCAC G^TGCAC
1578 GTGCAC
ApaLI 1011 GTGCAC G^TGCAC
1578 GTGCAC
BanI 456 GGTACC G^G[CT][GA]CC
756 GGCACC
BbvCI 771 GCTGAGG CCTCAGC(-5/-2)
1760 GCTGAGG
BfaI 486 CTAG C^TAG
1483 CTAG
BsaAI 1825 CACGTG [CT]AC^GT[GA]
1900 CACGTA
BsaWI 187 TCCGGA [AT]^CCGG[AT]
198 ACCGGT
BsrFI 198 ACCGGT [GA]^CCGG[CT]
1574 GCCGGT
BstXI 50 CCAGGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
394 CCAAGGAGTTGG
BstYI 475 AGATCC [GA]^GATC[CT]
539 GGATCT
Cfr10I 198 ACCGGT [GA]^CCGG[CT]
1574 GCCGGT
DsaI 1021 CCATGG C^C[GA][CT]GG
1123 CCATGG
HaeII 1295 GGCGCT [GA]GCGC^[CT]
1372 GGCGCT
HgaI 579 GACGC GACGC(5/10)
6 GCGTC
MaeI 486 CTAG C^TAG
1483 CTAG
MflI 475 AGATCC [GA]^GATC[CT]
539 GGATCT
NcoI 1021 CCATGG C^CATGG
1123 CCATGG
PaeR7I 1398 CTCGAG C^TCGAG
1588 CTCGAG
PshAI 945 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1528 GACCCTTGTC
XhoI 1398 CTCGAG C^TCGAG
1588 CTCGAG
XhoII 475 AGATCC [GA]^GATC[CT]
539 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BanII BbsI BbvI BcnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TseI Tsp45I
TspRI TthHB8I XcmI