##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII AccII AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AvrII BamHI BbeI BbrPI
BcgI BciVI BclI BfaI BfrI
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrGI BssHII Bst1107I BstAPI
BstUI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT22I FbaI FseI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KspI MaeI MfeI
MluI MroI MseI MunI MvnI
NarI NdeI NheI NotI NruI
NsiI NspI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI StuI SwaI
Tru9I Tsp509I Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 954 GGTACC G^GTACC
AccI 711 GTCTAC GT^[AC][GT]AC
AfaI 955 GTAC GT^AC
AlwNI 330 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1081 GGGCCC GGGCC^C
Asp718I 954 GGTACC G^GTACC
AvaI 685 CTCGGG C^[CT]CG[GA]G
AviII 103 TGCGCA TGC^GCA
BalI 602 TGGCCA TGG^CCA
BbvCI 666 CCTCAGC CCTCAGC(-5/-2)
BcnI 995 CCCGG CC^[GC]GG
BglI 591 GCCAACGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1150 AGATCT A^GATCT
BsaBI 1151 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI 1143 GTCTC GTCTC(1/5)
BsmBI 1142 CGTCTC CGTCTC(1/5)
BsoBI 685 CTCGGG C^[CT]CG[GA]G
BspHI 1215 TCATGA T^CATGA
BssSI 912 CACGAG CACGAG(-5/-1)
BstBI 862 TTCGAA TT^CGAA
BstEII 746 GGTGACC G^GT[ACGT]ACC
BstPI 746 GGTGACC G^GT[ACGT]ACC
CfoI 104 GCGC GCG^C
DrdI 1047 GACTTTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 77 CCTCAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 746 GGTGACC G^GT[ACGT]ACC
FspI 103 TGCGCA TGC^GCA
HhaI 104 GCGC GCG^C
HinP1I 104 GCGC G^CGC
KpnI 954 GGTACC GGTAC^C
MaeII 49 ACGT A^CGT
MamI 1151 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 602 TGGCCA TGG^CCA
MscI 602 TGGCCA TGG^CCA
MspA1I 437 CAGCTG C[AC]G^C[GT]G
NciI 995 CCCGG CC^[GC]GG
NspV 862 TTCGAA TT^CGAA
PpuMI 94 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 437 CAGCTG CAG^CTG
RcaI 1215 TCATGA T^CATGA
RsaI 955 GTAC GT^AC
SacI 1134 GAGCTC GAGCT^C
SapI 1136 GCTCTTC GCTCTTC(1/4)
SbfI 116 CCTGCAGG CCTGCA^GG
SfaNI 57 GATGC GCATC(5/9)
SfuI 862 TTCGAA TT^CGAA
Sse8387I 116 CCTGCAGG CCTGCA^GG
XcmI 832 CCAGTGGCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AspHI 675 GTGCTC G[AT]GC[AT]^C
1134 GAGCTC
BanI 209 GGTGCC G^G[CT][GA]CC
954 GGTACC
BbsI 203 GAAGAC GAAGAC(2/6)
1119 GAAGAC
BpuAI 203 GAAGAC GAAGAC(2/6)
1119 GAAGAC
BsgI 327 GTGCAG GTGCAG(16/14)
930 GTGCAG
BsiHKAI 675 GTGCTC G[AT]GC[AT]^C
1134 GAGCTC
BsrDI 544 CATTGC GCAATG(2/0)
106 GCAATG
BsrFI 39 GCCGGC [GA]^CCGG[CT]
442 GCCGGC
BstXI 421 CCAGGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
811 CCACCTTTTTGG
BstYI 535 AGATCC [GA]^GATC[CT]
1150 AGATCT
Cfr10I 39 GCCGGC [GA]^CCGG[CT]
442 GCCGGC
DraII 94 AGGACCT [GA]G^G[ACGT]CC[CT]
1080 GGGGCCC
EcoO109I 94 AGGACCT [GA]G^G[ACGT]CC[CT]
1080 GGGGCCC
FauI 24 CCCGC CCCGC(4/6)
902 GCGGG
MflI 535 AGATCC [GA]^GATC[CT]
1150 AGATCT
MslI 347 CACTGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
557 CACAGCCATG
NaeI 39 GCCGGC GCC^GGC
442 GCCGGC
NcoI 562 CCATGG C^CATGG
1195 CCATGG
NgoMIV 39 GCCGGC G^CCGGC
442 GCCGGC
PleI 1122 GACTC GAGTC(4/5)
1054 GAGTC
SmlI 78 CTCAAG C^T[CT][GA]AG
526 CTCAAG
TfiI 865 GAATC G^A[AT]TC
1034 GATTC
XhoII 535 AGATCC [GA]^GATC[CT]
1150 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AluI AlwI AvaII BanII
BbvI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DsaI EaeI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MnlI MspI MvaI MwoI NdeII
NlaIII NlaIV PstI Sau3AI Sau96I
ScrFI SfcI StyI TaqI TseI
Tsp45I TspRI TthHB8I