################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## AatII AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AvrII BamHI BbeI BbrPI BcgI BciVI BclI BfaI BfrI BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII Bst1107I BstAPI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FbaI FseI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KspI MaeI MfeI MluI MroI MseI MunI MvnI NarI NdeI NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI SspBI SspI StuI SwaI Tru9I Tsp509I Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 954 GGTACC G^GTACC AccI 711 GTCTAC GT^[AC][GT]AC AfaI 955 GTAC GT^AC AlwNI 330 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 1081 GGGCCC GGGCC^C Asp718I 954 GGTACC G^GTACC AvaI 685 CTCGGG C^[CT]CG[GA]G AviII 103 TGCGCA TGC^GCA BalI 602 TGGCCA TGG^CCA BbvCI 666 CCTCAGC CCTCAGC(-5/-2) BcnI 995 CCCGG CC^[GC]GG BglI 591 GCCAACGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1150 AGATCT A^GATCT BsaBI 1151 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmAI 1143 GTCTC GTCTC(1/5) BsmBI 1142 CGTCTC CGTCTC(1/5) BsoBI 685 CTCGGG C^[CT]CG[GA]G BspHI 1215 TCATGA T^CATGA BssSI 912 CACGAG CACGAG(-5/-1) BstBI 862 TTCGAA TT^CGAA BstEII 746 GGTGACC G^GT[ACGT]ACC BstPI 746 GGTGACC G^GT[ACGT]ACC CfoI 104 GCGC GCG^C DrdI 1047 GACTTTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 77 CCTCAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 746 GGTGACC G^GT[ACGT]ACC FspI 103 TGCGCA TGC^GCA HhaI 104 GCGC GCG^C HinP1I 104 GCGC G^CGC KpnI 954 GGTACC GGTAC^C MaeII 49 ACGT A^CGT MamI 1151 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 602 TGGCCA TGG^CCA MscI 602 TGGCCA TGG^CCA MspA1I 437 CAGCTG C[AC]G^C[GT]G NciI 995 CCCGG CC^[GC]GG NspV 862 TTCGAA TT^CGAA PpuMI 94 AGGACCT [GA]G^G[AT]CC[CT] PvuII 437 CAGCTG CAG^CTG RcaI 1215 TCATGA T^CATGA RsaI 955 GTAC GT^AC SacI 1134 GAGCTC GAGCT^C SapI 1136 GCTCTTC GCTCTTC(1/4) SbfI 116 CCTGCAGG CCTGCA^GG SfaNI 57 GATGC GCATC(5/9) SfuI 862 TTCGAA TT^CGAA Sse8387I 116 CCTGCAGG CCTGCA^GG XcmI 832 CCAGTGGCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AspHI 675 GTGCTC G[AT]GC[AT]^C 1134 GAGCTC BanI 209 GGTGCC G^G[CT][GA]CC 954 GGTACC BbsI 203 GAAGAC GAAGAC(2/6) 1119 GAAGAC BpuAI 203 GAAGAC GAAGAC(2/6) 1119 GAAGAC BsgI 327 GTGCAG GTGCAG(16/14) 930 GTGCAG BsiHKAI 675 GTGCTC G[AT]GC[AT]^C 1134 GAGCTC BsrDI 544 CATTGC GCAATG(2/0) 106 GCAATG BsrFI 39 GCCGGC [GA]^CCGG[CT] 442 GCCGGC BstXI 421 CCAGGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 811 CCACCTTTTTGG BstYI 535 AGATCC [GA]^GATC[CT] 1150 AGATCT Cfr10I 39 GCCGGC [GA]^CCGG[CT] 442 GCCGGC DraII 94 AGGACCT [GA]G^G[ACGT]CC[CT] 1080 GGGGCCC EcoO109I 94 AGGACCT [GA]G^G[ACGT]CC[CT] 1080 GGGGCCC FauI 24 CCCGC CCCGC(4/6) 902 GCGGG MflI 535 AGATCC [GA]^GATC[CT] 1150 AGATCT MslI 347 CACTGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 557 CACAGCCATG NaeI 39 GCCGGC GCC^GGC 442 GCCGGC NcoI 562 CCATGG C^CATGG 1195 CCATGG NgoMIV 39 GCCGGC G^CCGGC 442 GCCGGC PleI 1122 GACTC GAGTC(4/5) 1054 GAGTC SmlI 78 CTCAAG C^T[CT][GA]AG 526 CTCAAG TfiI 865 GAATC G^A[AT]TC 1034 GATTC XhoII 535 AGATCC [GA]^GATC[CT] 1150 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AciI AluI AlwI AvaII BanII BbvI BmyI BpmI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MspI MvaI MwoI NdeII NlaIII NlaIV PstI Sau3AI Sau96I ScrFI SfcI StyI TaqI TseI Tsp45I TspRI TthHB8I