##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI AlwI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII BamHI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BlpI Bpu1102I BsaAI BsaBI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstXI
BstZ17I CelII ClaI CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KpnI KspI
MaeII MamI MluI MslI MspA1I
NciI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacII SalI SbfI
SexAI SfiI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I XcmI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AccII 64 CGCG CG^CG
AccIII 987 TCCGGA T^CCGGA
AcsI 611 AAATTT [GA]^AATT[CT]
Alw44I 998 GTGCAC G^TGCAC
AlwNI 446 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 830 CCTGAGG CC^T[ACGT]AGG
ApaLI 998 GTGCAC G^TGCAC
ApoI 611 AAATTT [GA]^AATT[CT]
AvaI 1314 CTCGAG C^[CT]CG[GA]G
AvrII 55 CCTAGG C^CTAGG
BbeI 307 GGCGCC GGCGC^C
BglI 180 GCCTACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 913 AGATCT A^GATCT
BlnI 55 CCTAGG C^CTAGG
BsaI 382 GGTCTC GGTCTC(1/5)
BseAI 987 TCCGGA T^CCGGA
BsgI 887 CTGCAC GTGCAG(16/14)
BsmBI 259 CGTCTC CGTCTC(1/5)
BsoBI 1314 CTCGAG C^[CT]CG[GA]G
BspEI 987 TCCGGA T^CCGGA
BspMI 1374 GCAGGT ACCTGC(4/8)
BstBI 11 TTCGAA TT^CGAA
BstEII 1377 GGTGACC G^GT[ACGT]ACC
BstPI 1377 GGTGACC G^GT[ACGT]ACC
BstUI 64 CGCG CG^CG
BstYI 913 AGATCT [GA]^GATC[CT]
Bsu36I 830 CCTGAGG CC^T[ACGT]AGG
DpnII 914 GATC ^GATC
DraIII 890 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1025 GACCCCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 853 CCGTGG C^C[GA][CT]GG
Eco81I 830 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1377 GGTGACC G^GT[ACGT]ACC
FauI 94 CCCGC CCCGC(4/6)
HincII 461 GTTGAC GT[CT]^[GA]AC
HindII 461 GTTGAC GT[CT]^[GA]AC
KasI 307 GGCGCC G^GCGCC
MboI 914 GATC ^GATC
MfeI 501 CAATTG C^AATTG
MflI 913 AGATCT [GA]^GATC[CT]
MroI 987 TCCGGA T^CCGGA
MunI 501 CAATTG C^AATTG
MvnI 64 CGCG CG^CG
NaeI 82 GCCGGC GCC^GGC
NarI 307 GGCGCC GG^CGCC
NdeII 914 GATC ^GATC
NgoMIV 82 GCCGGC G^CCGGC
NspV 11 TTCGAA TT^CGAA
PaeR7I 1314 CTCGAG C^TCGAG
SacI 1407 GAGCTC GAGCT^C
SapI 14 GAAGAGC GCTCTTC(1/4)
Sau3AI 914 GATC ^GATC
ScaI 491 AGTACT AGT^ACT
SfcI 1077 CTGTAG C^T[GA][CT]AG
SfoI 307 GGCGCC GGC^GCC
SfuI 11 TTCGAA TT^CGAA
SspI 423 AATATT AAT^ATT
StuI 1342 AGGCCT AGG^CCT
TfiI 1073 GAATC G^A[AT]TC
XbaI 707 TCTAGA T^CTAGA
XhoI 1314 CTCGAG C^TCGAG
XhoII 913 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 307 GGCGCC G[GA]^CG[CT]C
810 GACGCC
AfaI 492 GTAC GT^AC
784 GTAC
ApaI 753 GGGCCC GGGCC^C
791 GGGCCC
BalI 129 TGGCCA TGG^CCA
881 TGGCCA
BbsI 824 GAAGAC GAAGAC(2/6)
1167 GTCTTC
BpuAI 824 GAAGAC GAAGAC(2/6)
1167 GTCTTC
BsaHI 307 GGCGCC G[GA]^CG[CT]C
810 GACGCC
BsaWI 987 TCCGGA [AT]^CCGG[AT]
1304 ACCGGA
BsrFI 82 GCCGGC [GA]^CCGG[CT]
579 ACCGGC
BsrI 98 CCAGT ACTGG(1/-1)
893 CCAGT
Cfr10I 82 GCCGGC [GA]^CCGG[CT]
579 ACCGGC
EarI 14 GAAGAG CTCTTC(1/4)
226 GAAGAG
EcoT14I 55 CCTAGG C^C[AT][AT]GG
648 CCAAGG
HaeII 114 AGCGCC [GA]GCGC^[CT]
307 GGCGCC
Hin1I 307 GGCGCC G[GA]^CG[CT]C
810 GACGCC
Ksp632I 14 GAAGAG CTCTTC(1/4)
226 GAAGAG
MluNI 129 TGGCCA TGG^CCA
881 TGGCCA
MscI 129 TGGCCA TGG^CCA
881 TGGCCA
NlaIII 733 CATG CATG^
1048 CATG
PflMI 121 CCATCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
445 CCAGCCCCTGG
PpuMI 1260 AGGACCT [GA]G^G[AT]CC[CT]
1321 AGGACCC
RsaI 492 GTAC GT^AC
784 GTAC
SfaNI 155 GCATC GCATC(5/9)
932 GCATC
SmlI 1133 CTTGAG C^T[CT][GA]AG
1314 CTCGAG
StyI 55 CCTAGG C^C[AT][AT]GG
648 CCAAGG
Van91I 121 CCATCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
445 CCAGCCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AluI AspHI AvaII BanI
BanII BbvI BfaI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DraII EaeI EcoNI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeIII MboII MnlI
MseI MspI MvaI MwoI NlaIV
PleI Sau96I ScrFI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I