################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## AatII Acc65I AccI AclI AflII AflIII AgeI AhdI AlwI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII BamHI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BlpI Bpu1102I BsaAI BsaBI BsiEI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstXI BstZ17I CelII ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI KspI MaeII MamI MluI MslI MspA1I NciI NcoI NdeI NheI NotI NruI NsiI NspI PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SbfI SexAI SfiI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AccII 64 CGCG CG^CG AccIII 987 TCCGGA T^CCGGA AcsI 611 AAATTT [GA]^AATT[CT] Alw44I 998 GTGCAC G^TGCAC AlwNI 446 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 830 CCTGAGG CC^T[ACGT]AGG ApaLI 998 GTGCAC G^TGCAC ApoI 611 AAATTT [GA]^AATT[CT] AvaI 1314 CTCGAG C^[CT]CG[GA]G AvrII 55 CCTAGG C^CTAGG BbeI 307 GGCGCC GGCGC^C BglI 180 GCCTACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 913 AGATCT A^GATCT BlnI 55 CCTAGG C^CTAGG BsaI 382 GGTCTC GGTCTC(1/5) BseAI 987 TCCGGA T^CCGGA BsgI 887 CTGCAC GTGCAG(16/14) BsmBI 259 CGTCTC CGTCTC(1/5) BsoBI 1314 CTCGAG C^[CT]CG[GA]G BspEI 987 TCCGGA T^CCGGA BspMI 1374 GCAGGT ACCTGC(4/8) BstBI 11 TTCGAA TT^CGAA BstEII 1377 GGTGACC G^GT[ACGT]ACC BstPI 1377 GGTGACC G^GT[ACGT]ACC BstUI 64 CGCG CG^CG BstYI 913 AGATCT [GA]^GATC[CT] Bsu36I 830 CCTGAGG CC^T[ACGT]AGG DpnII 914 GATC ^GATC DraIII 890 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1025 GACCCCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 853 CCGTGG C^C[GA][CT]GG Eco81I 830 CCTGAGG CC^T[ACGT]AGG EcoO65I 1377 GGTGACC G^GT[ACGT]ACC FauI 94 CCCGC CCCGC(4/6) HincII 461 GTTGAC GT[CT]^[GA]AC HindII 461 GTTGAC GT[CT]^[GA]AC KasI 307 GGCGCC G^GCGCC MboI 914 GATC ^GATC MfeI 501 CAATTG C^AATTG MflI 913 AGATCT [GA]^GATC[CT] MroI 987 TCCGGA T^CCGGA MunI 501 CAATTG C^AATTG MvnI 64 CGCG CG^CG NaeI 82 GCCGGC GCC^GGC NarI 307 GGCGCC GG^CGCC NdeII 914 GATC ^GATC NgoMIV 82 GCCGGC G^CCGGC NspV 11 TTCGAA TT^CGAA PaeR7I 1314 CTCGAG C^TCGAG SacI 1407 GAGCTC GAGCT^C SapI 14 GAAGAGC GCTCTTC(1/4) Sau3AI 914 GATC ^GATC ScaI 491 AGTACT AGT^ACT SfcI 1077 CTGTAG C^T[GA][CT]AG SfoI 307 GGCGCC GGC^GCC SfuI 11 TTCGAA TT^CGAA SspI 423 AATATT AAT^ATT StuI 1342 AGGCCT AGG^CCT TfiI 1073 GAATC G^A[AT]TC XbaI 707 TCTAGA T^CTAGA XhoI 1314 CTCGAG C^TCGAG XhoII 913 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 307 GGCGCC G[GA]^CG[CT]C 810 GACGCC AfaI 492 GTAC GT^AC 784 GTAC ApaI 753 GGGCCC GGGCC^C 791 GGGCCC BalI 129 TGGCCA TGG^CCA 881 TGGCCA BbsI 824 GAAGAC GAAGAC(2/6) 1167 GTCTTC BpuAI 824 GAAGAC GAAGAC(2/6) 1167 GTCTTC BsaHI 307 GGCGCC G[GA]^CG[CT]C 810 GACGCC BsaWI 987 TCCGGA [AT]^CCGG[AT] 1304 ACCGGA BsrFI 82 GCCGGC [GA]^CCGG[CT] 579 ACCGGC BsrI 98 CCAGT ACTGG(1/-1) 893 CCAGT Cfr10I 82 GCCGGC [GA]^CCGG[CT] 579 ACCGGC EarI 14 GAAGAG CTCTTC(1/4) 226 GAAGAG EcoT14I 55 CCTAGG C^C[AT][AT]GG 648 CCAAGG HaeII 114 AGCGCC [GA]GCGC^[CT] 307 GGCGCC Hin1I 307 GGCGCC G[GA]^CG[CT]C 810 GACGCC Ksp632I 14 GAAGAG CTCTTC(1/4) 226 GAAGAG MluNI 129 TGGCCA TGG^CCA 881 TGGCCA MscI 129 TGGCCA TGG^CCA 881 TGGCCA NlaIII 733 CATG CATG^ 1048 CATG PflMI 121 CCATCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 445 CCAGCCCCTGG PpuMI 1260 AGGACCT [GA]G^G[AT]CC[CT] 1321 AGGACCC RsaI 492 GTAC GT^AC 784 GTAC SfaNI 155 GCATC GCATC(5/9) 932 GCATC SmlI 1133 CTTGAG C^T[CT][GA]AG 1314 CTCGAG StyI 55 CCTAGG C^C[AT][AT]GG 648 CCAAGG Van91I 121 CCATCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 445 CCAGCCCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AciI AluI AspHI AvaII BanI BanII BbvI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DraII EaeI EcoNI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboII MnlI MseI MspI MvaI MwoI NlaIV PleI Sau96I ScrFI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I