##################################
# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BcnI BfrI BlnI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BspEI BspLU11I BsrBI
BsrDI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
CfoI CpoI DraI DraIII EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoT14I FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII KasI KpnI KspI
MamI MluI MluNI MroI MscI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NotI NruI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI StyI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AflIII 495 ACGTGT A^C[GA][CT]GT
AlwNI 1102 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 141 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 606 CTCGGG C^[CT]CG[GA]G
BanII 165 GGGCTC G[GA]GC[CT]^C
BbsI 1242 GTCTTC GAAGAC(2/6)
BclI 1094 TGATCA T^GATCA
BglI 442 GCCGTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 66 AGATCT A^GATCT
BlpI 691 GCTGAGC GC^T[ACGT]AGC
BmyI 165 GGGCTC G[AGT]GC[ACT]^C
BpmI 928 CTGGAG CTGGAG(16/14)
Bpu1102I 691 GCTGAGC GC^T[ACGT]AGC
BpuAI 1242 GTCTTC GAAGAC(2/6)
BsaAI 494 TACGTG [CT]AC^GT[GA]
BseRI 947 CTCCTC GAGGAG(10/8)
BsgI 506 CTGCAC GTGCAG(16/14)
BsiYI 1101 CCAGCTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1101 CCAGCTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 945 GTCTC GTCTC(1/5)
BsmI 256 GAATGC GAATGC(1/-1)
BsoBI 606 CTCGGG C^[CT]CG[GA]G
Bsp1286I 165 GGGCTC G[AGT]GC[ACT]^C
BspDI 1020 ATCGAT AT^CGAT
BsrFI 959 ACCGGC [GA]^CCGG[CT]
BstAPI 16 GCAACTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 691 GCTGAGC GC^T[ACGT]AGC
Cfr10I 959 ACCGGC [GA]^CCGG[CT]
ClaI 1020 ATCGAT AT^CGAT
DrdI 1204 GACCCTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 443 CCGTGG C^C[GA][CT]GG
EarI 561 CTCTTC CTCTTC(1/4)
EcoRV 54 GATATC GAT^ATC
EcoT22I 272 ATGCAT ATGCA^T
FauI 670 GCGGG CCCGC(4/6)
FbaI 1094 TGATCA T^GATCA
HapII 960 CCGG C^CGG
HpaI 643 GTTAAC GTT^AAC
HpaII 960 CCGG C^CGG
Ksp632I 561 CTCTTC CTCTTC(1/4)
MfeI 729 CAATTG C^AATTG
MslI 1064 CATTAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1102 CAGCTG C[AC]G^C[GT]G
MspI 960 CCGG C^CGG
MunI 729 CAATTG C^AATTG
NheI 937 GCTAGC G^CTAGC
NsiI 272 ATGCAT ATGCA^T
PflMI 1101 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 42 GAGTC GAGTC(4/5)
PpuMI 1202 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 1102 CAGCTG CAG^CTG
TaqI 1021 TCGA T^CGA
TthHB8I 1021 TCGA T^CGA
Van91I 1101 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 141 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AciI 670 GCGG CCGC(-3/-1)
963 GCGG
AcsI 324 GAATTT [GA]^AATT[CT]
1260 AAATTC
AocI 1198 CCTCAGG CC^T[ACGT]AGG
1219 CCTCAGG
ApoI 324 GAATTT [GA]^AATT[CT]
1260 AAATTC
BfaI 791 CTAG C^TAG
938 CTAG
BsmFI 956 GGGAC GGGAC(10/14)
838 GTCCC
Bsp1407I 98 TGTACA T^GTACA
917 TGTACA
BspHI 1154 TCATGA T^CATGA
1229 TCATGA
BspMI 654 ACCTGC ACCTGC(4/8)
696 GCAGGT
BsrGI 98 TGTACA T^GTACA
917 TGTACA
BsrI 927 ACTGG ACTGG(1/-1)
594 CCAGT
BstF5I 396 GGATG GGATG(2/0)
1110 GGATG
Bsu36I 1198 CCTCAGG CC^T[ACGT]AGG
1219 CCTCAGG
DraII 737 AGGCCCC [GA]G^G[ACGT]CC[CT]
1202 AGGACCC
Eco81I 1198 CCTCAGG CC^T[ACGT]AGG
1219 CCTCAGG
EcoO109I 737 AGGCCCC [GA]G^G[ACGT]CC[CT]
1202 AGGACCC
FokI 396 GGATG GGATG(9/13)
1110 GGATG
HincII 220 GTCAAC GT[CT]^[GA]AC
643 GTTAAC
HindII 220 GTCAAC GT[CT]^[GA]AC
643 GTTAAC
MaeI 791 CTAG C^TAG
938 CTAG
MaeII 495 ACGT A^CGT
1240 ACGT
RcaI 1154 TCATGA T^CATGA
1229 TCATGA
SspBI 98 TGTACA T^GTACA
917 TGTACA
TfiI 382 GAATC G^A[AT]TC
1233 GAATC
Tsp45I 370 GTGAC ^GT[GC]AC
1213 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AfaI AluI AlwI AvaII BanI
BbvI BsaJI BssKI BstNI BstYI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI HaeIII HinfI HphI ItaI
MaeIII MboI MboII MflI MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI Tru9I TseI Tsp509I TspRI
XhoII