################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BlnI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BspEI BspLU11I BsrBI BsrDI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I CfoI CpoI DraI DraIII EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoT14I FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII KasI KpnI KspI MamI MluI MluNI MroI MscI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AflIII 495 ACGTGT A^C[GA][CT]GT AlwNI 1102 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 141 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 606 CTCGGG C^[CT]CG[GA]G BanII 165 GGGCTC G[GA]GC[CT]^C BbsI 1242 GTCTTC GAAGAC(2/6) BclI 1094 TGATCA T^GATCA BglI 442 GCCGTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 66 AGATCT A^GATCT BlpI 691 GCTGAGC GC^T[ACGT]AGC BmyI 165 GGGCTC G[AGT]GC[ACT]^C BpmI 928 CTGGAG CTGGAG(16/14) Bpu1102I 691 GCTGAGC GC^T[ACGT]AGC BpuAI 1242 GTCTTC GAAGAC(2/6) BsaAI 494 TACGTG [CT]AC^GT[GA] BseRI 947 CTCCTC GAGGAG(10/8) BsgI 506 CTGCAC GTGCAG(16/14) BsiYI 1101 CCAGCTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 1101 CCAGCTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 945 GTCTC GTCTC(1/5) BsmI 256 GAATGC GAATGC(1/-1) BsoBI 606 CTCGGG C^[CT]CG[GA]G Bsp1286I 165 GGGCTC G[AGT]GC[ACT]^C BspDI 1020 ATCGAT AT^CGAT BsrFI 959 ACCGGC [GA]^CCGG[CT] BstAPI 16 GCAACTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 691 GCTGAGC GC^T[ACGT]AGC Cfr10I 959 ACCGGC [GA]^CCGG[CT] ClaI 1020 ATCGAT AT^CGAT DrdI 1204 GACCCTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 443 CCGTGG C^C[GA][CT]GG EarI 561 CTCTTC CTCTTC(1/4) EcoRV 54 GATATC GAT^ATC EcoT22I 272 ATGCAT ATGCA^T FauI 670 GCGGG CCCGC(4/6) FbaI 1094 TGATCA T^GATCA HapII 960 CCGG C^CGG HpaI 643 GTTAAC GTT^AAC HpaII 960 CCGG C^CGG Ksp632I 561 CTCTTC CTCTTC(1/4) MfeI 729 CAATTG C^AATTG MslI 1064 CATTAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 1102 CAGCTG C[AC]G^C[GT]G MspI 960 CCGG C^CGG MunI 729 CAATTG C^AATTG NheI 937 GCTAGC G^CTAGC NsiI 272 ATGCAT ATGCA^T PflMI 1101 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 42 GAGTC GAGTC(4/5) PpuMI 1202 AGGACCC [GA]G^G[AT]CC[CT] PvuII 1102 CAGCTG CAG^CTG TaqI 1021 TCGA T^CGA TthHB8I 1021 TCGA T^CGA Van91I 1101 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 141 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AciI 670 GCGG CCGC(-3/-1) 963 GCGG AcsI 324 GAATTT [GA]^AATT[CT] 1260 AAATTC AocI 1198 CCTCAGG CC^T[ACGT]AGG 1219 CCTCAGG ApoI 324 GAATTT [GA]^AATT[CT] 1260 AAATTC BfaI 791 CTAG C^TAG 938 CTAG BsmFI 956 GGGAC GGGAC(10/14) 838 GTCCC Bsp1407I 98 TGTACA T^GTACA 917 TGTACA BspHI 1154 TCATGA T^CATGA 1229 TCATGA BspMI 654 ACCTGC ACCTGC(4/8) 696 GCAGGT BsrGI 98 TGTACA T^GTACA 917 TGTACA BsrI 927 ACTGG ACTGG(1/-1) 594 CCAGT BstF5I 396 GGATG GGATG(2/0) 1110 GGATG Bsu36I 1198 CCTCAGG CC^T[ACGT]AGG 1219 CCTCAGG DraII 737 AGGCCCC [GA]G^G[ACGT]CC[CT] 1202 AGGACCC Eco81I 1198 CCTCAGG CC^T[ACGT]AGG 1219 CCTCAGG EcoO109I 737 AGGCCCC [GA]G^G[ACGT]CC[CT] 1202 AGGACCC FokI 396 GGATG GGATG(9/13) 1110 GGATG HincII 220 GTCAAC GT[CT]^[GA]AC 643 GTTAAC HindII 220 GTCAAC GT[CT]^[GA]AC 643 GTTAAC MaeI 791 CTAG C^TAG 938 CTAG MaeII 495 ACGT A^CGT 1240 ACGT RcaI 1154 TCATGA T^CATGA 1229 TCATGA SspBI 98 TGTACA T^GTACA 917 TGTACA TfiI 382 GAATC G^A[AT]TC 1233 GAATC Tsp45I 370 GTGAC ^GT[GC]AC 1213 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AfaI AluI AlwI AvaII BanI BbvI BsaJI BssKI BstNI BstYI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII HinfI HphI ItaI MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI Tru9I TseI Tsp509I TspRI XhoII