##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
Acc65I AccIII AclI Alw44I AocI
ApaI ApaLI AscI AseI AsnI
Asp718I AviII AvrII BalI BamHI
BbeI BbrPI BcgI BciVI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco52I Eco81I EcoO65I EcoRI EcoT22I
FseI FspI HincII HindII HpaI
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI StuI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AatII 29 GACGTC GACGT^C
AccI 1536 GTCTAC GT^[AC][GT]AC
AccII 228 CGCG CG^CG
AcsI 981 AAATTT [GA]^AATT[CT]
AcyI 29 GACGTC G[GA]^CG[CT]C
AflII 924 CTTAAG C^TTAAG
AflIII 1402 ACATGT A^C[GA][CT]GT
AgeI 916 ACCGGT A^CCGGT
AhdI 561 GACGGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 78 AGCGCT AGC^GCT
ApoI 981 AAATTT [GA]^AATT[CT]
AspEI 561 GACGGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 524 GGTGCC G^G[CT][GA]CC
BanII 1509 GAGCCC G[GA]GC[CT]^C
BbsI 1095 GAAGAC GAAGAC(2/6)
BfrI 924 CTTAAG C^TTAAG
BpuAI 1095 GAAGAC GAAGAC(2/6)
BsaBI 1626 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 29 GACGTC G[GA]^CG[CT]C
BsaI 142 GGTCTC GGTCTC(1/5)
BsaWI 916 ACCGGT [AT]^CCGG[AT]
BsgI 1165 CTGCAC GTGCAG(16/14)
BsmBI 4 CGTCTC CGTCTC(1/5)
BspHI 1685 TCATGA T^CATGA
BspLU11I 1402 ACATGT A^CATGT
BspMI 1099 ACCTGC ACCTGC(4/8)
BsrBI 1366 CCGCTC CCGCTC(-3/-3)
BstUI 228 CGCG CG^CG
BstXI 796 CCATCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 500 AGATCC [GA]^GATC[CT]
DraII 1447 AGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 1265 TGGCCG [CT]^GGCC[GA]
Eam1105I 561 GACGGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 78 AGCGCT AGC^GCT
EcoO109I 1447 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 621 GATATC GAT^ATC
HgaI 682 GACGC GACGC(5/10)
Hin1I 29 GACGTC G[GA]^CG[CT]C
MamI 1626 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 500 AGATCC [GA]^GATC[CT]
MslI 1724 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 723 CAGCTG C[AC]G^C[GT]G
MvnI 228 CGCG CG^CG
NaeI 183 GCCGGC GCC^GGC
NgoMIV 183 GCCGGC G^CCGGC
PaeR7I 145 CTCGAG C^TCGAG
PflMI 86 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 916 ACCGGT A^CCGGT
PpuMI 1447 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 723 CAGCTG CAG^CTG
RcaI 1685 TCATGA T^CATGA
SexAI 1531 ACCAGGT A^CC[AT]GGT
SfcI 393 CTACAG C^T[GA][CT]AG
SmaI 1512 CCCGGG CCC^GGG
SphI 1189 GCATGC GCATG^C
Tsp45I 32 GTCAC ^GT[GC]AC
Van91I 86 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 934 CCATCAATATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 145 CTCGAG C^TCGAG
XhoII 500 AGATCC [GA]^GATC[CT]
XmaCI 1512 CCCGGG C^CCGGG
XmaI 1512 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Asp700I 237 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
319 GAAGCTTTTC
AspHI 1173 GAGCAC G[AT]GC[AT]^C
1707 GAGCAC
AspI 101 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1530 GACCAGGTC
AvaI 145 CTCGAG C^[CT]CG[GA]G
1512 CCCGGG
BbvCI 720 CCTCAGC CCTCAGC(-5/-2)
1193 GCTGAGG
BclI 301 TGATCA T^GATCA
1688 TGATCA
BsiHKAI 1173 GAGCAC G[AT]GC[AT]^C
1707 GAGCAC
BsoBI 145 CTCGAG C^[CT]CG[GA]G
1512 CCCGGG
BssSI 1168 CACGAG CACGAG(-5/-1)
1200 CACGAG
DsaI 36 CCGTGG C^C[GA][CT]GG
1248 CCGTGG
EarI 1419 CTCTTC CTCTTC(1/4)
291 GAAGAG
FbaI 301 TGATCA T^GATCA
1688 TGATCA
HaeII 78 AGCGCT [GA]GCGC^[CT]
537 GGCGCT
Ksp632I 1419 CTCTTC CTCTTC(1/4)
291 GAAGAG
MaeII 30 ACGT A^CGT
102 ACGT
PflFI 101 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1530 GACCAGGTC
SspI 939 AATATT AAT^ATT
1155 AATATT
Tsp509I 982 AATT ^AATT
1645 AATT
Tth111I 101 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1530 GACCAGGTC
XmnI 237 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
319 GAAGCTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII EcoNI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MnlI MseI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
StyI TaqI TfiI Tru9I TseI
TspRI TthHB8I