##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BalI BbrPI BcgI BciVI BclI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HpaI MamI MfeI MluI MluNI
MscI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SalI SapI SbfI ScaI
SfcI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 1184 GGTACC G^GTACC
AccII 31 CGCG CG^CG
AccIII 576 TCCGGA T^CCGGA
AflIII 417 ACGTGT A^C[GA][CT]GT
AlwNI 854 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 307 GGGCCC GGGCC^C
Asp718I 1184 GGTACC G^GTACC
AspHI 1301 GAGCTC G[AT]GC[AT]^C
BamHI 776 GGATCC G^GATCC
BbeI 208 GGCGCC GGCGC^C
BbvCI 851 CCTCAGC CCTCAGC(-5/-2)
BfaI 834 CTAG C^TAG
BglII 989 AGATCT A^GATCT
BsaI 480 GAGACC GGTCTC(1/5)
BsaWI 576 TCCGGA [AT]^CCGG[AT]
BseAI 576 TCCGGA T^CCGGA
BsiHKAI 1301 GAGCTC G[AT]GC[AT]^C
BspEI 576 TCCGGA T^CCGGA
BstUI 31 CGCG CG^CG
CfoI 209 GCGC GCG^C
EaeI 663 TGGCCG [CT]^GGCC[GA]
EcoT14I 768 CCATGG C^C[AT][AT]GG
HaeII 208 GGCGCC [GA]GCGC^[CT]
HhaI 209 GCGC GCG^C
HinP1I 209 GCGC G^CGC
HindIII 522 AAGCTT A^AGCTT
KasI 208 GGCGCC G^GCGCC
KpnI 1184 GGTACC GGTAC^C
KspI 30 CCGCGG CCGC^GG
MaeI 834 CTAG C^TAG
MroI 576 TCCGGA T^CCGGA
MslI 901 CACGAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 30 CCGCGG C[AC]G^C[GT]G
MvnI 31 CGCG CG^CG
NarI 208 GGCGCC GG^CGCC
NcoI 768 CCATGG C^CATGG
PflMI 950 CCAGACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 881 GACTC GAGTC(4/5)
PpuMI 114 GGGACCC [GA]G^G[AT]CC[CT]
SacI 1301 GAGCTC GAGCT^C
SacII 30 CCGCGG CCGC^GG
SexAI 1287 ACCAGGT A^CC[AT]GGT
SfoI 208 GGCGCC GGC^GCC
SmlI 1416 CTCAAG C^T[CT][GA]AG
StyI 768 CCATGG C^C[AT][AT]GG
TfiI 1206 GAATC G^A[AT]TC
Van91I 950 CCAGACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 24 GGCGTC G[GA]^CG[CT]C
208 GGCGCC
AvaI 241 CCCGAG C^[CT]CG[GA]G
1242 CTCGGG
BanI 208 GGCGCC G^G[CT][GA]CC
1184 GGTACC
BbvI 1007 GCAGC GCAGC(8/12)
442 GCTGC
BsaHI 24 GGCGTC G[GA]^CG[CT]C
208 GGCGCC
BsoBI 241 CCCGAG C^[CT]CG[GA]G
1242 CTCGGG
BsrBI 283 GAGCGG CCGCTC(-3/-3)
796 GAGCGG
BsrDI 108 GCAATG GCAATG(2/0)
725 GCAATG
BsrFI 1 GCCGGT [GA]^CCGG[CT]
79 GCCGGT
BssSI 149 CACGAG CACGAG(-5/-1)
446 CACGAG
BstYI 776 GGATCC [GA]^GATC[CT]
989 AGATCT
Cfr10I 1 GCCGGT [GA]^CCGG[CT]
79 GCCGGT
DraI 526 TTTAAA TTT^AAA
1473 TTTAAA
DraII 114 GGGACCC [GA]G^G[ACGT]CC[CT]
306 GGGGCCC
EcoO109I 114 GGGACCC [GA]G^G[ACGT]CC[CT]
306 GGGGCCC
HgaI 25 GCGTC GACGC(5/10)
752 GCGTC
Hin1I 24 GGCGTC G[GA]^CG[CT]C
208 GGCGCC
HincII 820 GTCAAC GT[CT]^[GA]AC
1076 GTTGAC
HindII 820 GTCAAC GT[CT]^[GA]AC
1076 GTTGAC
HinfI 881 GACTC G^A[ACGT]TC
1206 GAATC
MaeII 417 ACGT A^CGT
605 ACGT
MflI 776 GGATCC [GA]^GATC[CT]
989 AGATCT
StuI 734 AGGCCT AGG^CCT
1446 AGGCCT
TaqI 171 TCGA T^CGA
926 TCGA
TseI 442 GCTGC G^C[AT]GC
1007 GCAGC
Tsp509I 332 AATT ^AATT
803 AATT
TthHB8I 171 TCGA T^CGA
926 TCGA
XhoII 776 GGATCC [GA]^GATC[CT]
989 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanII BbsI BcnI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DsaI EarI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MseI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI Tru9I Tsp45I TspRI