##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AccIII AflII AflIII AgeI AhdI
Alw44I ApaI ApaLI AscI AseI
AsnI AspEI AviII AvrII BamHI
BbeI BbvCI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaBI BsaWI
BseAI BseRI BsiWI BsmBI BspDI
BspEI BspLU11I BssHII Bst1107I BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI Eam1105I EcoO65I EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KspI MamI
MfeI MluI MroI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI RsrII SacII SalI
SbfI ScaI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 577 GACGTC GACGT^C
Acc65I 2328 GGTACC G^GTACC
AccI 593 GTCTAC GT^[AC][GT]AC
AclI 680 AACGTT AA^CGTT
AcyI 577 GACGTC G[GA]^CG[CT]C
AocI 2594 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1963 AGCGCT AGC^GCT
Asp718I 2328 GGTACC G^GTACC
AspI 944 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 2298 CACGTG CAC^GTG
BcgI 2236 CGACGATGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 893 GCCATGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1067 AGATCT A^GATCT
BsaAI 2298 CACGTG [CT]AC^GT[GA]
BsaHI 577 GACGTC G[GA]^CG[CT]C
BsaI 1556 GAGACC GGTCTC(1/5)
BsgI 1409 GTGCAG GTGCAG(16/14)
BsmI 373 GAATGC GAATGC(1/-1)
BssSI 2627 CACGAG CACGAG(-5/-1)
BstAPI 382 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2594 CCTCAGG CC^T[ACGT]AGG
DraIII 1245 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1122 CGGCCG C^GGCCG
EclXI 1122 CGGCCG C^GGCCG
Eco47III 1963 AGCGCT AGC^GCT
Eco52I 1122 CGGCCG C^GGCCG
Eco81I 2594 CCTCAGG CC^T[ACGT]AGG
EcoRV 2504 GATATC GAT^ATC
HgaI 1473 GCGTC GACGC(5/10)
Hin1I 577 GACGTC G[GA]^CG[CT]C
KpnI 2328 GGTACC GGTAC^C
NaeI 503 GCCGGC GCC^GGC
NgoMIV 503 GCCGGC G^CCGGC
NspI 1428 ACATGC [GA]CATG^[CT]
PflFI 944 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2132 CCAGACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2298 CACGTG CAC^GTG
PmlI 2298 CACGTG CAC^GTG
Psp1406I 680 AACGTT AA^CGTT
PvuI 1135 CGATCG CGAT^CG
SacI 221 GAGCTC GAGCT^C
SexAI 945 ACCTGGT A^CC[AT]GGT
SfiI 892 GGCCATGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tth111I 944 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2132 CCAGACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Asp700I 278 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1500 GAAGTCTTTC
BbsI 1262 GAAGAC GAAGAC(2/6)
128 GTCTTC
BfaI 1071 CTAG C^TAG
2764 CTAG
BpuAI 1262 GAAGAC GAAGAC(2/6)
128 GTCTTC
BsiEI 1122 CGGCCG CG[GA][CT]^CG
1135 CGATCG
Bsp1407I 870 TGTACA T^GTACA
2685 TGTACA
BspHI 6 TCATGA T^CATGA
2034 TCATGA
BsrGI 870 TGTACA T^GTACA
2685 TGTACA
BstXI 984 CCAACTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1887 CCAAATTCCTGG
DrdI 638 GACCCTTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2135 GACATTGGTGTC
EcoNI 1075 CCTTCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2071 CCTGAACAAGG
EcoRI 1196 GAATTC G^AATTC
1617 GAATTC
HaeII 1963 AGCGCT [GA]GCGC^[CT]
2408 GGCGCT
MaeI 1071 CTAG C^TAG
2764 CTAG
MseI 1017 TTAA T^TAA
1587 TTAA
NcoI 894 CCATGG C^CATGG
2394 CCATGG
PstI 392 CTGCAG CTGCA^G
1433 CTGCAG
RcaI 6 TCATGA T^CATGA
2034 TCATGA
SmaI 1313 CCCGGG CCC^GGG
1828 CCCGGG
SmlI 341 CTCAAG C^T[CT][GA]AG
398 CTCAAG
SspBI 870 TGTACA T^GTACA
2685 TGTACA
TfiI 259 GATTC G^A[AT]TC
862 GAATC
Tru9I 1017 TTAA T^TAA
1587 TTAA
XmaCI 1313 CCCGGG C^CCGGG
1828 CCCGGG
XmaI 1313 CCCGGG C^CCGGG
1828 CCCGGG
XmnI 278 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1500 GAAGTCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanI BanII BbvI
BcnI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII