##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AgeI AscI AseI AsnI
Asp700I Asp718I AspI AviII BamHI
BbeI BcgI BfrI BsaBI BsaHI
BsiEI BsiWI BspDI BspHI BssHII
BssSI Bst1107I BstBI BstUI BstZ17I
ClaI CpoI DrdI EagI EclXI
Eco52I EcoRI EcoT22I FseI FspI
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SapI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI SspI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 1429 TCCGGA T^CCGGA
Aor51HI 2795 AGCGCT AGC^GCT
ApaI 2526 GGGCCC GGGCC^C
AvrII 2156 CCTAGG C^CTAGG
BbrPI 1194 CACGTG CAC^GTG
BglI 1900 GCCAGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1864 AGATCT A^GATCT
BlnI 2156 CCTAGG C^CTAGG
BseAI 1429 TCCGGA T^CCGGA
BsmBI 1089 GAGACG CGTCTC(1/5)
BsmI 2057 GAATGC GAATGC(1/-1)
BspEI 1429 TCCGGA T^CCGGA
BspLU11I 2262 ACATGT A^CATGT
BstAPI 3371 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 286 GGTAACC G^GT[ACGT]ACC
BstPI 286 GGTAACC G^GT[ACGT]ACC
DraI 678 TTTAAA TTT^AAA
Eco47III 2795 AGCGCT AGC^GCT
EcoO65I 286 GGTAACC G^GT[ACGT]ACC
EcoRV 1404 GATATC GAT^ATC
FauI 2341 CCCGC CCCGC(4/6)
MroI 1429 TCCGGA T^CCGGA
NspI 2262 ACATGT [GA]CATG^[CT]
PmaCI 1194 CACGTG CAC^GTG
PmlI 1194 CACGTG CAC^GTG
PshAI 2974 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 1120 GTCGAC G^TCGAC
SbfI 2734 CCTGCAGG CCTGCA^GG
SmaI 1411 CCCGGG CCC^GGG
Sse8387I 2734 CCTGCAGG CCTGCA^GG
StuI 1950 AGGCCT AGG^CCT
XmaCI 1411 CCCGGG C^CCGGG
XmaI 1411 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AflIII 958 ACGTGT A^C[GA][CT]GT
2262 ACATGT
AhdI 1044 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1091 GACGAAGGGTC
Alw44I 1970 GTGCAC G^TGCAC
3451 GTGCAC
AocI 2915 CCTCAGG CC^T[ACGT]AGG
3498 CCTGAGG
ApaLI 1970 GTGCAC G^TGCAC
3451 GTGCAC
AspEI 1044 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1091 GACGAAGGGTC
AvaI 1411 CCCGGG C^[CT]CG[GA]G
3017 CTCGGG
BalI 11 TGGCCA TGG^CCA
3001 TGGCCA
BbsI 1491 GAAGAC GAAGAC(2/6)
2921 GAAGAC
BciVI 2258 GGATAC GTATCC(6/5)
1019 GTATCC
BclI 613 TGATCA T^GATCA
947 TGATCA
BpuAI 1491 GAAGAC GAAGAC(2/6)
2921 GAAGAC
BsaAI 957 TACGTG [CT]AC^GT[GA]
1194 CACGTG
BsaWI 1429 TCCGGA [AT]^CCGG[AT]
1572 ACCGGA
BsoBI 1411 CCCGGG C^[CT]CG[GA]G
3017 CTCGGG
BsrFI 1532 GCCGGT [GA]^CCGG[CT]
1746 GCCGGT
BstXI 2215 CCATCATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2983 CCAGTCCTCTGG
BstYI 1864 AGATCT [GA]^GATC[CT]
2076 GGATCT
Bsu36I 2915 CCTCAGG CC^T[ACGT]AGG
3498 CCTGAGG
Cfr10I 1532 GCCGGT [GA]^CCGG[CT]
1746 GCCGGT
EaeI 11 TGGCCA [CT]^GGCC[GA]
3001 TGGCCA
Eam1105I 1044 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1091 GACGAAGGGTC
EarI 725 CTCTTC CTCTTC(1/4)
3009 CTCTTC
Eco81I 2915 CCTCAGG CC^T[ACGT]AGG
3498 CCTGAGG
FbaI 613 TGATCA T^GATCA
947 TGATCA
HaeII 1716 AGCGCC [GA]GCGC^[CT]
2795 AGCGCT
HincII 175 GTCAAC GT[CT]^[GA]AC
1120 GTCGAC
HindII 175 GTCAAC GT[CT]^[GA]AC
1120 GTCGAC
Ksp632I 725 CTCTTC CTCTTC(1/4)
3009 CTCTTC
MflI 1864 AGATCT [GA]^GATC[CT]
2076 GGATCT
MluNI 11 TGGCCA TGG^CCA
3001 TGGCCA
MscI 11 TGGCCA TGG^CCA
3001 TGGCCA
PpuMI 1675 GGGACCC [GA]G^G[AT]CC[CT]
2855 GGGTCCC
ScaI 1186 AGTACT AGT^ACT
2461 AGTACT
XhoII 1864 AGATCT [GA]^GATC[CT]
2076 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BanI BanII BbvCI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspMI BsrBI BsrDI BsrGI
BsrI BssKI BstF5I BstNI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I