##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI AspEI AspI
AvrII BcgI BfrI BlnI BsaBI
BsaWI BseAI BsiWI BspDI BspEI
BspLU11I BssHII BstBI BstXI Bsu36I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRV
EcoT22I FseI HpaI KspI MamI
MfeI MroI MunI NaeI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SbfI ScaI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 1421 GGTACC G^GTACC
AflIII 2685 ACGCGT A^C[GA][CT]GT
ApaI 2570 GGGCCC GGGCC^C
Asp718I 1421 GGTACC G^GTACC
AviII 308 TGCGCA TGC^GCA
BamHI 1778 GGATCC G^GATCC
BbeI 2531 GGCGCC GGCGC^C
BbrPI 544 CACGTG CAC^GTG
BglII 1528 AGATCT A^GATCT
BlpI 1833 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1833 GCTCAGC GC^T[ACGT]AGC
BsaAI 544 CACGTG [CT]AC^GT[GA]
BsiEI 3 CGACCG CG[GA][CT]^CG
BsmI 2917 GCATTC GAATGC(1/-1)
Bsp1407I 1472 TGTACA T^GTACA
BspHI 911 TCATGA T^CATGA
BsrBI 2983 GAGCGG CCGCTC(-3/-3)
BsrDI 1982 CATTGC GCAATG(2/0)
BsrFI 2397 ACCGGC [GA]^CCGG[CT]
BsrGI 1472 TGTACA T^GTACA
BssSI 1186 CTCGTG CACGAG(-5/-1)
Bst1107I 2971 GTATAC GTA^TAC
BstEII 1606 GGTTACC G^GT[ACGT]ACC
BstPI 1606 GGTTACC G^GT[ACGT]ACC
BstZ17I 2971 GTATAC GTA^TAC
CelII 1833 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2397 ACCGGC [GA]^CCGG[CT]
DrdI 940 GACAAGGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 507 CCTCTTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1606 GGTTACC G^GT[ACGT]ACC
EcoRI 619 GAATTC G^AATTC
FspI 308 TGCGCA TGC^GCA
HincII 1 GTCGAC GT[CT]^[GA]AC
HindII 1 GTCGAC GT[CT]^[GA]AC
HindIII 1573 AAGCTT A^AGCTT
KasI 2531 GGCGCC G^GCGCC
KpnI 1421 GGTACC GGTAC^C
MluI 2685 ACGCGT A^CGCGT
NarI 2531 GGCGCC GG^CGCC
NheI 2172 GCTAGC G^CTAGC
NspI 82 ACATGC [GA]CATG^[CT]
PmaCI 544 CACGTG CAC^GTG
PmlI 544 CACGTG CAC^GTG
RcaI 911 TCATGA T^CATGA
SacI 1867 GAGCTC GAGCT^C
SalI 1 GTCGAC G^TCGAC
SfoI 2531 GGCGCC GGC^GCC
SspBI 1472 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 946 GGCGTC G[GA]^CG[CT]C
2531 GGCGCC
BbsI 583 GAAGAC GAAGAC(2/6)
55 GTCTTC
BbvCI 778 GCTGAGG CCTCAGC(-5/-2)
2995 GCTGAGG
BciVI 485 GTATCC GTATCC(6/5)
2809 GTATCC
BclI 1697 TGATCA T^GATCA
2862 TGATCA
BpuAI 583 GAAGAC GAAGAC(2/6)
55 GTCTTC
BsaHI 946 GGCGTC G[GA]^CG[CT]C
2531 GGCGCC
BsaI 1890 GAGACC GGTCTC(1/5)
2232 GGTCTC
BsmBI 2729 CGTCTC CGTCTC(1/5)
3147 GAGACG
DraIII 270 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
1456 CACCAGGTG
FbaI 1697 TGATCA T^GATCA
2862 TGATCA
HaeII 1166 AGCGCC [GA]GCGC^[CT]
2531 GGCGCC
Hin1I 946 GGCGTC G[GA]^CG[CT]C
2531 GGCGCC
PaeR7I 868 CTCGAG C^TCGAG
1504 CTCGAG
SexAI 1457 ACCAGGT A^CC[AT]GGT
1582 ACCTGGT
StuI 101 AGGCCT AGG^CCT
1450 AGGCCT
XhoI 868 CTCGAG C^TCGAG
1504 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbvI BcnI BfaI BglI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI