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# NO CUT ENZYMES ( 99 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp718I AviII AvrII
BamHI BbeI BbrPI BbvCI BclI
BcnI BfrI BglI BlnI BsaAI
BsaBI BsaI BsiEI BsiWI BsmBI
BsmFI BspDI BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I Cfr10I ClaI
CpoI EagI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV FauI FbaI
FseI FspI HpaI KasI KpnI
KspI MamI MluI MspA1I NaeI
NarI NciI NgoMIV NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI PvuII
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SwaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AatII 2147 GACGTC GACGT^C
AccII 2717 CGCG CG^CG
AccIII 1422 TCCGGA T^CCGGA
AflIII 1408 ACATGT A^C[GA][CT]GT
AhdI 2142 GACTTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 805 ATTAAT AT^TAAT
AsnI 805 ATTAAT AT^TAAT
AspEI 2142 GACTTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1730 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2439 CTCGAG C^[CT]CG[GA]G
BalI 2604 TGGCCA TGG^CCA
BanI 1991 GGTGCC G^G[CT][GA]CC
BcgI 1469 GCACATCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 588 GTATCC GTATCC(6/5)
BlpI 746 GCTGAGC GC^T[ACGT]AGC
BpmI 1289 CTGGAG CTGGAG(16/14)
Bpu1102I 746 GCTGAGC GC^T[ACGT]AGC
BseAI 1422 TCCGGA T^CCGGA
BsgI 1467 CTGCAC GTGCAG(16/14)
BsmI 607 GAATGC GAATGC(1/-1)
BsoBI 2439 CTCGAG C^[CT]CG[GA]G
Bsp1407I 2008 TGTACA T^GTACA
BspEI 1422 TCCGGA T^CCGGA
BspLU11I 1408 ACATGT A^CATGT
BsrGI 2008 TGTACA T^GTACA
BstUI 2717 CGCG CG^CG
CelII 746 GCTGAGC GC^T[ACGT]AGC
DraI 1082 TTTAAA TTT^AAA
DraII 2236 GGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 1374 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1334 GACAAAGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2142 GACTTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2422 CCTTCGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 2236 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 2777 ATGCAT ATGCA^T
HaeII 2263 GGCGCT [GA]GCGC^[CT]
HgaI 988 GACGC GACGC(5/10)
HincII 81 GTCAAC GT[CT]^[GA]AC
HindII 81 GTCAAC GT[CT]^[GA]AC
HindIII 2027 AAGCTT A^AGCTT
MfeI 378 CAATTG C^AATTG
MluNI 2604 TGGCCA TGG^CCA
MroI 1422 TCCGGA T^CCGGA
MscI 2604 TGGCCA TGG^CCA
MunI 378 CAATTG C^AATTG
MvnI 2717 CGCG CG^CG
NdeI 155 CATATG CA^TATG
NheI 2305 GCTAGC G^CTAGC
NsiI 2777 ATGCAT ATGCA^T
NspI 1408 ACATGT [GA]CATG^[CT]
PaeR7I 2439 CTCGAG C^TCGAG
PflFI 1730 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2582 CCAACGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2236 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 805 ATTAAT AT^TAAT
SacI 1903 GAGCTC GAGCT^C
SpeI 2281 ACTAGT A^CTAGT
SspBI 2008 TGTACA T^GTACA
Tth111I 1730 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2582 CCAACGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 923 TCTAGA T^CTAGA
XhoI 2439 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AciI 1118 GCGG CCGC(-3/-1)
2354 GCGG
AcyI 988 GACGCC G[GA]^CG[CT]C
2147 GACGTC
BanII 919 GGGCTC G[GA]GC[CT]^C
1903 GAGCTC
BbsI 1759 GAAGAC GAAGAC(2/6)
2372 GAAGAC
BpuAI 1759 GAAGAC GAAGAC(2/6)
2372 GAAGAC
BsaHI 988 GACGCC G[GA]^CG[CT]C
2147 GACGTC
BsaWI 1360 TCCGGT [AT]^CCGG[AT]
1422 TCCGGA
BspHI 46 TCATGA T^CATGA
1623 TCATGA
BspMI 1434 ACCTGC ACCTGC(4/8)
2531 GCAGGT
BsrDI 1383 GCAATG GCAATG(2/0)
2399 GCAATG
CfoI 2264 GCGC GCG^C
2716 GCGC
DsaI 1581 CCATGG C^C[GA][CT]GG
2364 CCATGG
EaeI 2355 CGGCCA [CT]^GGCC[GA]
2604 TGGCCA
EcoRI 252 GAATTC G^AATTC
1488 GAATTC
HapII 1361 CCGG C^CGG
1423 CCGG
HhaI 2264 GCGC GCG^C
2716 GCGC
Hin1I 988 GACGCC G[GA]^CG[CT]C
2147 GACGTC
HinP1I 2264 GCGC G^CGC
2716 GCGC
HpaII 1361 CCGG C^CGG
1423 CCGG
MaeII 1091 ACGT A^CGT
2148 ACGT
MspI 1361 CCGG C^CGG
1423 CCGG
MwoI 1109 GCAGAGTTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
2309 GCTGGTGCTGC
NcoI 1581 CCATGG C^CATGG
2364 CCATGG
PstI 1043 CTGCAG CTGCA^G
1632 CTGCAG
RcaI 46 TCATGA T^CATGA
1623 TCATGA
SmlI 1888 CTTGAG C^T[CT][GA]AG
2439 CTCGAG
SspI 617 AATATT AAT^ATT
1857 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I AspHI AvaII BbvI BfaI
BglII BmyI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MvaI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI