##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccI AclI AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
AspI AvrII BalI BamHI BcgI
BclI BlnI BsiWI BspDI BspMI
BsrBI BsrDI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraI EagI
EclXI Eco47III Eco52I EcoRV EcoT22I
FbaI FseI HindIII HpaI MluI
MluNI MscI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI SbfI
ScaI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 1712 GGTACC G^GTACC
AccIII 108 TCCGGA T^CCGGA
AflII 878 CTTAAG C^TTAAG
AflIII 529 ACATGT A^C[GA][CT]GT
AhdI 746 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1678 CCTTAGG CC^T[ACGT]AGG
Asp700I 1219 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1712 GGTACC G^GTACC
AspEI 746 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2721 TGCGCA TGC^GCA
BbeI 564 GGCGCC GGCGC^C
BciVI 1061 GTATCC GTATCC(6/5)
BfrI 878 CTTAAG C^TTAAG
BglI 1688 GCCCTTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2943 AGATCT A^GATCT
BsaWI 108 TCCGGA [AT]^CCGG[AT]
BseAI 108 TCCGGA T^CCGGA
BsiEI 324 CGACCG CG[GA][CT]^CG
BsmBI 763 CGTCTC CGTCTC(1/5)
BsmI 231 GCATTC GAATGC(1/-1)
Bsp1407I 2664 TGTACA T^GTACA
BspEI 108 TCCGGA T^CCGGA
BspHI 2623 TCATGA T^CATGA
BspLU11I 529 ACATGT A^CATGT
BsrGI 2664 TGTACA T^GTACA
BssSI 937 CACGAG CACGAG(-5/-1)
BstAPI 2779 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1678 CCTTAGG CC^T[ACGT]AGG
DrdI 2159 GACTCCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 271 TGGCCG [CT]^GGCC[GA]
Eam1105I 746 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1678 CCTTAGG CC^T[ACGT]AGG
EcoRI 1468 GAATTC G^AATTC
FspI 2721 TGCGCA TGC^GCA
HincII 2831 GTTGAC GT[CT]^[GA]AC
HindII 2831 GTTGAC GT[CT]^[GA]AC
KasI 564 GGCGCC G^GCGCC
KpnI 1712 GGTACC GGTAC^C
KspI 486 CCGCGG CCGC^GG
MfeI 282 CAATTG C^AATTG
MroI 108 TCCGGA T^CCGGA
MunI 282 CAATTG C^AATTG
NaeI 379 GCCGGC GCC^GGC
NarI 564 GGCGCC GG^CGCC
NcoI 268 CCATGG C^CATGG
NgoMIV 379 GCCGGC G^CCGGC
NspI 529 ACATGT [GA]CATG^[CT]
RcaI 2623 TCATGA T^CATGA
SacII 486 CCGCGG CCGC^GG
SapI 2956 GAAGAGC GCTCTTC(1/4)
SfoI 564 GGCGCC GGC^GCC
SmlI 878 CTTAAG C^T[CT][GA]AG
SspBI 2664 TGTACA T^GTACA
StuI 2973 AGGCCT AGG^CCT
XmnI 1219 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 41 CGCG CG^CG
487 CGCG
AcsI 1220 AAATTT [GA]^AATT[CT]
1468 GAATTC
ApoI 1220 AAATTT [GA]^AATT[CT]
1468 GAATTC
BbrPI 905 CACGTG CAC^GTG
2985 CACGTG
BbsI 653 GTCTTC GAAGAC(2/6)
1153 GTCTTC
BlpI 226 GCTGAGC GC^T[ACGT]AGC
2788 GCTGAGC
Bpu1102I 226 GCTGAGC GC^T[ACGT]AGC
2788 GCTGAGC
BpuAI 653 GTCTTC GAAGAC(2/6)
1153 GTCTTC
BsaBI 1008 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2843 GATGAGGATC
BsaI 307 GAGACC GGTCTC(1/5)
2082 GGTCTC
BstUI 41 CGCG CG^CG
487 CGCG
BstXI 1001 CCAACAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2027 CCAGAAGGATGG
BstYI 1706 GGATCT [GA]^GATC[CT]
2943 AGATCT
CelII 226 GCTGAGC GC^T[ACGT]AGC
2788 GCTGAGC
EarI 2867 GAAGAG CTCTTC(1/4)
2956 GAAGAG
Ksp632I 2867 GAAGAG CTCTTC(1/4)
2956 GAAGAG
MamI 1008 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2843 GATGAGGATC
MflI 1706 GGATCT [GA]^GATC[CT]
2943 AGATCT
MvnI 41 CGCG CG^CG
487 CGCG
PmaCI 905 CACGTG CAC^GTG
2985 CACGTG
PmlI 905 CACGTG CAC^GTG
2985 CACGTG
SacI 913 GAGCTC GAGCT^C
2531 GAGCTC
TaqI 323 TCGA T^CGA
2954 TCGA
TfiI 320 GATTC G^A[AT]TC
2651 GAATC
TthHB8I 323 TCGA T^CGA
2954 TCGA
XhoII 1706 GGATCT [GA]^GATC[CT]
2943 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbvCI BbvI BcnI
BfaI BmyI BpmI BsaAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BstEII BstF5I
BstNI BstPI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI StyI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I