##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII AclI AcyI AflII AflIII
Aor51HI AscI AseI AsnI AspI
AviII AvrII BbeI BbrPI BciVI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsmBI BsmI BspDI
BspHI BspLU11I BsrBI BssHII BssSI
Bst1107I BstBI BstZ17I CelII ClaI
CpoI DraI DrdI Eco47III FspI
HaeII HgaI Hin1I HpaI KasI
KspI MamI MfeI MluI MunI
NarI NheI NotI NruI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 2728 GGTACC G^GTACC
AccI 385 GTCGAC GT^[AC][GT]AC
AccII 252 CGCG CG^CG
AccIII 100 TCCGGA T^CCGGA
AgeI 362 ACCGGT A^CCGGT
AhdI 312 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2440 CCTCAGG CC^T[ACGT]AGG
Asp700I 2345 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2728 GGTACC G^GTACC
AspEI 312 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2858 CTCGGG C^[CT]CG[GA]G
BalI 2405 TGGCCA TGG^CCA
BamHI 1713 GGATCC G^GATCC
BcgI 387 CGACTGGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2660 TGATCA T^GATCA
BglII 517 AGATCT A^GATCT
BsaI 910 GAGACC GGTCTC(1/5)
BseAI 100 TCCGGA T^CCGGA
BsiEI 1544 CGGCCG CG[GA][CT]^CG
BsiWI 89 CGTACG C^GTACG
BsoBI 2858 CTCGGG C^[CT]CG[GA]G
BspEI 100 TCCGGA T^CCGGA
BstAPI 1671 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 252 CGCG CG^CG
Bsu36I 2440 CCTCAGG CC^T[ACGT]AGG
DraIII 1891 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1544 CGGCCG C^GGCCG
Eam1105I 312 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1544 CGGCCG C^GGCCG
Eco52I 1544 CGGCCG C^GGCCG
Eco81I 2440 CCTCAGG CC^T[ACGT]AGG
EcoNI 2483 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 584 GAATTC G^AATTC
EcoRV 2810 GATATC GAT^ATC
EcoT22I 564 ATGCAT ATGCA^T
FbaI 2660 TGATCA T^GATCA
FseI 1485 GGCCGGCC GGCCGG^CC
HindIII 49 AAGCTT A^AGCTT
KpnI 2728 GGTACC GGTAC^C
MaeII 2164 ACGT A^CGT
MluNI 2405 TGGCCA TGG^CCA
MroI 100 TCCGGA T^CCGGA
MscI 2405 TGGCCA TGG^CCA
MvnI 252 CGCG CG^CG
NaeI 1486 GCCGGC GCC^GGC
NdeI 567 CATATG CA^TATG
NgoMIV 1486 GCCGGC G^CCGGC
NsiI 564 ATGCAT ATGCA^T
PinAI 362 ACCGGT A^CCGGT
SalI 385 GTCGAC G^TCGAC
SapI 575 GCTCTTC GCTCTTC(1/4)
TfiI 750 GAATC G^A[AT]TC
XmnI 2345 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 499 AAATTT [GA]^AATT[CT]
584 GAATTC
Alw44I 889 GTGCAC G^TGCAC
2509 GTGCAC
AlwNI 622 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
1269 CAGGCCCTG
ApaLI 889 GTGCAC G^TGCAC
2509 GTGCAC
ApoI 499 AAATTT [GA]^AATT[CT]
584 GAATTC
BcnI 1299 CCGGG CC^[GC]GG
1699 CCCGG
BglI 1225 GCCATGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1486 GCCGGCCAGGC
BsaWI 100 TCCGGA [AT]^CCGG[AT]
362 ACCGGT
BsmAI 910 GAGAC GTCTC(1/5)
1068 GTCTC
Bsp1407I 160 TGTACA T^GTACA
1585 TGTACA
BsrDI 713 CATTGC GCAATG(2/0)
2645 GCAATG
BsrFI 362 ACCGGT [GA]^CCGG[CT]
1486 GCCGGC
BsrGI 160 TGTACA T^GTACA
1585 TGTACA
BstEII 491 GGTCACC G^GT[ACGT]ACC
2267 GGTGACC
BstPI 491 GGTCACC G^GT[ACGT]ACC
2267 GGTGACC
BstXI 1208 CCACCACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1442 CCATGCCCATGG
Cfr10I 362 ACCGGT [GA]^CCGG[CT]
1486 GCCGGC
EcoO65I 491 GGTCACC G^GT[ACGT]ACC
2267 GGTGACC
HincII 228 GTTGAC GT[CT]^[GA]AC
385 GTCGAC
HindII 228 GTTGAC GT[CT]^[GA]AC
385 GTCGAC
NciI 1299 CCGGG CC^[GC]GG
1699 CCCGG
NspI 1277 GCATGC [GA]CATG^[CT]
2753 GCATGC
PflMI 26 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
437 CCAAAGGCTGG
SacI 2359 GAGCTC GAGCT^C
2389 GAGCTC
SmlI 879 CTCAAG C^T[CT][GA]AG
2569 CTTGAG
SphI 1277 GCATGC GCATG^C
2753 GCATGC
SspBI 160 TGTACA T^GTACA
1585 TGTACA
StuI 1156 AGGCCT AGG^CCT
2208 AGGCCT
Tsp509I 500 AATT ^AATT
585 AATT
Van91I 26 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
437 CCAAAGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AfaI AluI AlwI ApaI
AspHI AvaII BanI BanII BbsI
BbvCI BbvI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI Tru9I TseI Tsp45I
TspRI TthHB8I XcmI XhoII