##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AhdI Alw44I AocI ApaLI AscI
AseI AsnI AspEI AviII AvrII
BbeI BciVI BclI BfrI BlnI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrGI
BssHII Bst1107I BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoT22I FbaI FseI FspI
HpaI KasI KspI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NotI NruI NsiI PacI
PaeR7I PmeI PshAI PshBI Psp1406I
PvuI RsrII SacII SapI SbfI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 588 GGTACC G^GTACC
AcyI 742 GACGCC G[GA]^CG[CT]C
AgeI 3353 ACCGGT A^CCGGT
Aor51HI 1880 AGCGCT AGC^GCT
Asp718I 588 GGTACC G^GTACC
AspI 3280 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 759 GGATCC G^GATCC
BbvCI 180 GCTGAGG CCTCAGC(-5/-2)
BcgI 2259 CGATATCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1128 AGATCT A^GATCT
BlpI 707 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 707 GCTCAGC GC^T[ACGT]AGC
BsaBI 504 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 742 GACGCC G[GA]^CG[CT]C
BsmI 2734 GCATTC GAATGC(1/-1)
BspHI 515 TCATGA T^CATGA
BsrDI 2265 CATTGC GCAATG(2/0)
BsrFI 3353 ACCGGT [GA]^CCGG[CT]
BstAPI 3165 GCAGCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1542 TTCGAA TT^CGAA
CelII 707 GCTCAGC GC^T[ACGT]AGC
Cfr10I 3353 ACCGGT [GA]^CCGG[CT]
EarI 805 GAAGAG CTCTTC(1/4)
Eco47III 1880 AGCGCT AGC^GCT
EcoRI 2953 GAATTC G^AATTC
EcoRV 2260 GATATC GAT^ATC
HaeII 1880 AGCGCT [GA]GCGC^[CT]
Hin1I 742 GACGCC G[GA]^CG[CT]C
HindIII 370 AAGCTT A^AGCTT
KpnI 588 GGTACC GGTAC^C
Ksp632I 805 GAAGAG CTCTTC(1/4)
MamI 504 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 1309 GCTAGC G^CTAGC
NspI 2787 GCATGT [GA]CATG^[CT]
NspV 1542 TTCGAA TT^CGAA
PflFI 3280 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3353 ACCGGT A^CCGGT
RcaI 515 TCATGA T^CATGA
SacI 1951 GAGCTC GAGCT^C
SalI 2619 GTCGAC G^TCGAC
ScaI 272 AGTACT AGT^ACT
SexAI 3281 ACCAGGT A^CC[AT]GGT
SfuI 1542 TTCGAA TT^CGAA
Tth111I 3280 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 527 CCAAGAAACTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 1704 GTCTAC GT^[AC][GT]AC
2619 GTCGAC
AccII 286 CGCG CG^CG
1068 CGCG
AcsI 631 GAATTT [GA]^AATT[CT]
2953 GAATTC
ApoI 631 GAATTT [GA]^AATT[CT]
2953 GAATTC
AvaI 1053 CCCGAG C^[CT]CG[GA]G
1667 CCCGAG
BalI 212 TGGCCA TGG^CCA
2123 TGGCCA
BbsI 690 GAAGAC GAAGAC(2/6)
1381 GAAGAC
BpuAI 690 GAAGAC GAAGAC(2/6)
1381 GAAGAC
BsaI 1565 GGTCTC GGTCTC(1/5)
1774 GGTCTC
BsaWI 338 ACCGGA [AT]^CCGG[AT]
3353 ACCGGT
BsoBI 1053 CCCGAG C^[CT]CG[GA]G
1667 CCCGAG
BspMI 1262 ACCTGC ACCTGC(4/8)
1020 GCAGGT
BstUI 286 CGCG CG^CG
1068 CGCG
DraIII 1900 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
2718 CACCCGGTG
HincII 1245 GTCAAC GT[CT]^[GA]AC
2619 GTCGAC
HindII 1245 GTCAAC GT[CT]^[GA]AC
2619 GTCGAC
MluNI 212 TGGCCA TGG^CCA
2123 TGGCCA
MscI 212 TGGCCA TGG^CCA
2123 TGGCCA
MseI 1046 TTAA T^TAA
2324 TTAA
MvnI 286 CGCG CG^CG
1068 CGCG
PflMI 2384 CCAGGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2853 CCATGTTCTGG
SfiI 607 GGCCGTGACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3204 GGCCGAGAAGGCC
Tru9I 1046 TTAA T^TAA
2324 TTAA
Van91I 2384 CCAGGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2853 CCATGTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI Asp700I AspHI AvaII BanI
BanII BbrPI BbvI BcnI BfaI
BglI BmyI BpmI BsaAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BssSI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI PmaCI PmlI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI