##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I BamHI
BciVI BclI BfrI BglI BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI Bst1107I BstBI BstZ17I ClaI
CpoI DraI EagI EclXI Eco47III
Eco52I EcoRI FbaI FseI HpaI
KpnI MamI MfeI MluI MroI
MunI NaeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SfiI
SfuI SgrAI SnaBI SphI Sse8387I
SspBI SspI SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AccI 1408 GTAGAC GT^[AC][GT]AC
AhdI 1012 GACAGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 2353 GGGCCC GGGCC^C
AspEI 1012 GACAGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1156 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 552 TGCGCA TGC^GCA
BbvCI 2961 GCTGAGG CCTCAGC(-5/-2)
BcgI 1977 CGACGCCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1087 AGATCT A^GATCT
BsrBI 331 GAGCGG CCGCTC(-3/-3)
DrdI 3397 GACTTGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1012 GACAGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1616 GATATC GAT^ATC
EcoT22I 2475 ATGCAT ATGCA^T
FspI 552 TGCGCA TGC^GCA
KspI 50 CCGCGG CCGC^GG
NdeI 2472 CATATG CA^TATG
NsiI 2475 ATGCAT ATGCA^T
PflFI 1156 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1303 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 50 CCGCGG CCGC^GG
SmaI 3034 CCCGGG CCC^GGG
SpeI 862 ACTAGT A^CTAGT
StuI 1692 AGGCCT AGG^CCT
Tth111I 1156 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2268 TCTAGA T^CTAGA
XmaCI 3034 CCCGGG C^CCGGG
XmaI 3034 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AflIII 744 ACGTGT A^C[GA][CT]GT
1708 ACGTGT
AocI 606 CCTTAGG CC^T[ACGT]AGG
1664 CCTCAGG
AvaI 2104 CCCGAG C^[CT]CG[GA]G
3034 CCCGGG
AvrII 1741 CCTAGG C^CTAGG
2133 CCTAGG
BbrPI 90 CACGTG CAC^GTG
1707 CACGTG
BbsI 3133 GAAGAC GAAGAC(2/6)
2869 GTCTTC
BlnI 1741 CCTAGG C^CTAGG
2133 CCTAGG
BlpI 328 GCTGAGC GC^T[ACGT]AGC
523 GCTGAGC
Bpu1102I 328 GCTGAGC GC^T[ACGT]AGC
523 GCTGAGC
BpuAI 3133 GAAGAC GAAGAC(2/6)
2869 GTCTTC
BsaAI 90 CACGTG [CT]AC^GT[GA]
1707 CACGTG
BsaI 673 GAGACC GGTCTC(1/5)
714 GAGACC
BsoBI 2104 CCCGAG C^[CT]CG[GA]G
3034 CCCGGG
BsrFI 125 ACCGGC [GA]^CCGG[CT]
2091 ACCGGC
BssHII 40 GCGCGC G^CGCGC
95 GCGCGC
BssSI 483 CACGAG CACGAG(-5/-1)
2228 CTCGTG
BstEII 1962 GGTGACC G^GT[ACGT]ACC
2164 GGTAACC
BstPI 1962 GGTGACC G^GT[ACGT]ACC
2164 GGTAACC
BstXI 1022 CCACTCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1241 CCAACTTAGTGG
Bsu36I 606 CCTTAGG CC^T[ACGT]AGG
1664 CCTCAGG
CelII 328 GCTGAGC GC^T[ACGT]AGC
523 GCTGAGC
Cfr10I 125 ACCGGC [GA]^CCGG[CT]
2091 ACCGGC
DraIII 250 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
3198 CACACTGTG
Eco81I 606 CCTTAGG CC^T[ACGT]AGG
1664 CCTCAGG
EcoO65I 1962 GGTGACC G^GT[ACGT]ACC
2164 GGTAACC
HincII 174 GTTGAC GT[CT]^[GA]AC
2911 GTCAAC
HindII 174 GTTGAC GT[CT]^[GA]AC
2911 GTCAAC
MseI 2150 TTAA T^TAA
2810 TTAA
MslI 2468 CACACATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2808 CATTAAGATG
PmaCI 90 CACGTG CAC^GTG
1707 CACGTG
PmlI 90 CACGTG CAC^GTG
1707 CACGTG
SacI 2138 GAGCTC GAGCT^C
2322 GAGCTC
SexAI 251 ACCAGGT A^CC[AT]GGT
3041 ACCTGGT
TaqI 5 TCGA T^CGA
503 TCGA
Tru9I 2150 TTAA T^TAA
2810 TTAA
TthHB8I 5 TCGA T^CGA
503 TCGA
XcmI 509 CCACTAGCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1505 CCAGACCCCTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaII BalI BanI BanII BbeI
BbvI BcnI BfaI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspMI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HindIII HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
StyI TfiI TseI Tsp45I Tsp509I
TspRI Van91I XhoII