##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
Acc65I AccI AccIII AclI AgeI
AhdI Aor51HI ApaI AscI Asp718I
AspEI AviII AvrII BamHI BbeI
BbvCI BlnI BsaBI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BsrGI BssHII BssSI Bst1107I BstEII
BstPI BstZ17I ClaI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoT22I FauI FseI
FspI KasI KpnI KspI MamI
MfeI MluI MroI MunI NarI
NdeI NheI NotI NruI NsiI
PacI PinAI PmeI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SexAI SfiI SfoI SgrAI SnaBI
SpeI SphI SspBI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Alw44I 2126 GTGCAC G^TGCAC
AocI 2167 CCTGAGG CC^T[ACGT]AGG
ApaLI 2126 GTGCAC G^TGCAC
AseI 1539 ATTAAT AT^TAAT
AsnI 1539 ATTAAT AT^TAAT
Asp700I 1377 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 2126 GTGCAC G[AT]GC[AT]^C
AspI 2486 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 3043 GGCACC G^G[CT][GA]CC
BcgI 3742 GCACGTGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 2298 GCCCGAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2138 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2138 GCTTAGC GC^T[ACGT]AGC
BsaI 2491 GGTCTC GGTCTC(1/5)
BsaWI 3115 ACCGGA [AT]^CCGG[AT]
BsiHKAI 2126 GTGCAC G[AT]GC[AT]^C
BspHI 1316 TCATGA T^CATGA
BsrFI 3040 GCCGGC [GA]^CCGG[CT]
BstBI 3517 TTCGAA TT^CGAA
Bsu36I 2167 CCTGAGG CC^T[ACGT]AGG
CelII 2138 GCTTAGC GC^T[ACGT]AGC
Cfr10I 3040 GCCGGC [GA]^CCGG[CT]
DraI 1395 TTTAAA TTT^AAA
DraII 2163 GGGACCT [GA]G^G[ACGT]CC[CT]
Eco81I 2167 CCTGAGG CC^T[ACGT]AGG
EcoNI 2753 CCTTGTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 2163 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 1654 GATATC GAT^ATC
HpaI 3054 GTTAAC GTT^AAC
NaeI 3040 GCCGGC GCC^GGC
NcoI 3877 CCATGG C^CATGG
NgoMIV 3040 GCCGGC G^CCGGC
NspV 3517 TTCGAA TT^CGAA
PflFI 2486 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2163 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 2429 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1539 ATTAAT AT^TAAT
RcaI 1316 TCATGA T^CATGA
ScaI 2155 AGTACT AGT^ACT
SfuI 3517 TTCGAA TT^CGAA
SmaI 1421 CCCGGG CCC^GGG
SspI 3933 AATATT AAT^ATT
StuI 557 AGGCCT AGG^CCT
Tth111I 2486 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1421 CCCGGG C^CCGGG
XmaI 1421 CCCGGG C^CCGGG
XmnI 1377 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AatII 981 GACGTC GACGT^C
3030 GACGTC
AcsI 3600 AAATTT [GA]^AATT[CT]
3624 GAATTT
ApoI 3600 AAATTT [GA]^AATT[CT]
3624 GAATTT
BbrPI 670 CACGTG CAC^GTG
3743 CACGTG
BciVI 1138 GGATAC GTATCC(6/5)
1919 GGATAC
BclI 1830 TGATCA T^GATCA
3542 TGATCA
BglII 95 AGATCT A^GATCT
1236 AGATCT
BspLU11I 2010 ACATGT A^CATGT
2794 ACATGT
BstXI 2944 CCATGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3871 CCACCACCATGG
DrdI 189 GACCGACAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1566 GACCATGATGTC
EarI 2494 CTCTTC CTCTTC(1/4)
3161 CTCTTC
FbaI 1830 TGATCA T^GATCA
3542 TGATCA
HaeII 1053 GGCGCT [GA]GCGC^[CT]
3867 AGCGCC
HindIII 3180 AAGCTT A^AGCTT
3724 AAGCTT
Ksp632I 2494 CTCTTC CTCTTC(1/4)
3161 CTCTTC
PaeR7I 653 CTCGAG C^TCGAG
2338 CTCGAG
PflMI 591 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2618 CCAGTGCCTGG
PmaCI 670 CACGTG CAC^GTG
3743 CACGTG
PmlI 670 CACGTG CAC^GTG
3743 CACGTG
SbfI 891 CCTGCAGG CCTGCA^GG
1924 CCTGCAGG
Sse8387I 891 CCTGCAGG CCTGCA^GG
1924 CCTGCAGG
Van91I 591 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2618 CCAGTGCCTGG
XhoI 653 CTCGAG C^TCGAG
2338 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AcyI AfaI AflII
AflIII AluI AlwI AlwNI AvaI
AvaII BalI BanII BbsI BbvI
BcnI BfaI BfrI BmyI BpmI
BpuAI BsaAI BsaHI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII