##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
AhdI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AviII BamHI
BbeI BbrPI BfrI BlpI Bpu1102I
BsaI BsiEI BsiWI BsmI Bsp1407I
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstZ17I CelII CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoT22I FseI FspI
HpaI KasI KpnI KspI MfeI
MluI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI SspBI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 731 GTCTAC GT^[AC][GT]AC
AccIII 126 TCCGGA T^CCGGA
AocI 2934 CCTCAGG CC^T[ACGT]AGG
AspI 2138 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1303 CCTAGG C^CTAGG
BbvCI 2168 CCTCAGC CCTCAGC(-5/-2)
BciVI 1843 GGATAC GTATCC(6/5)
BclI 1728 TGATCA T^GATCA
BlnI 1303 CCTAGG C^CTAGG
BsaAI 1433 TACGTG [CT]AC^GT[GA]
BsaBI 1729 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 126 TCCGGA T^CCGGA
BspEI 126 TCCGGA T^CCGGA
BspHI 2823 TCATGA T^CATGA
BssSI 107 CTCGTG CACGAG(-5/-1)
Bsu36I 2934 CCTCAGG CC^T[ACGT]AGG
DraIII 1781 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2934 CCTCAGG CC^T[ACGT]AGG
EcoRV 2096 GATATC GAT^ATC
FbaI 1728 TGATCA T^GATCA
HincII 2453 GTTGAC GT[CT]^[GA]AC
HindII 2453 GTTGAC GT[CT]^[GA]AC
HindIII 460 AAGCTT A^AGCTT
MamI 1729 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 126 TCCGGA T^CCGGA
MslI 661 CACCATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1797 CCATGG C^CATGG
NspI 1722 ACATGC [GA]CATG^[CT]
PflFI 2138 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1163 GACGTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2823 TCATGA T^CATGA
SapI 416 GAAGAGC GCTCTTC(1/4)
SbfI 3080 CCTGCAGG CCTGCA^GG
Sse8387I 3080 CCTGCAGG CCTGCA^GG
SspI 3139 AATATT AAT^ATT
StuI 1197 AGGCCT AGG^CCT
Tth111I 2138 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1467 CCAGCTCCCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AatII 728 GACGTC GACGT^C
1790 GACGTC
Alw44I 1436 GTGCAC G^TGCAC
1778 GTGCAC
AlwI 2560 GATCC GGATC(4/5)
2992 GATCC
AlwNI 425 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
3371 CAGCTGCTG
ApaLI 1436 GTGCAC G^TGCAC
1778 GTGCAC
Asp700I 1294 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1880 GAAGGCGTTC
BalI 195 TGGCCA TGG^CCA
657 TGGCCA
BanI 628 GGTGCC G^G[CT][GA]CC
2879 GGCACC
BcgI 2238 CGAACTGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1947 GCAGGACCATCG
BfaI 1304 CTAG C^TAG
2943 CTAG
BglII 1911 AGATCT A^GATCT
3316 AGATCT
BsgI 2419 CTGCAC GTGCAG(16/14)
2571 CTGCAC
BsmBI 1241 GAGACG CGTCTC(1/5)
3251 GAGACG
BspDI 376 ATCGAT AT^CGAT
1577 ATCGAT
BsrBI 2127 CCGCTC CCGCTC(-3/-3)
419 GAGCGG
BstEII 2857 GGTCACC G^GT[ACGT]ACC
3123 GGTCACC
BstPI 2857 GGTCACC G^GT[ACGT]ACC
3123 GGTCACC
BstXI 1143 CCACCGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1281 CCAGGGAGCTGG
ClaI 376 ATCGAT AT^CGAT
1577 ATCGAT
EcoNI 1687 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3295 CCTTCAGAAGG
EcoO65I 2857 GGTCACC G^GT[ACGT]ACC
3123 GGTCACC
HaeII 282 AGCGCC [GA]GCGC^[CT]
2594 AGCGCC
MaeI 1304 CTAG C^TAG
2943 CTAG
MluNI 195 TGGCCA TGG^CCA
657 TGGCCA
MscI 195 TGGCCA TGG^CCA
657 TGGCCA
MseI 591 TTAA T^TAA
1560 TTAA
NaeI 1418 GCCGGC GCC^GGC
2907 GCCGGC
NgoMIV 1418 GCCGGC G^CCGGC
2907 GCCGGC
PaeR7I 351 CTCGAG C^TCGAG
3330 CTCGAG
PflMI 2335 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2637 CCAAGTTGTGG
SexAI 641 ACCAGGT A^CC[AT]GGT
2139 ACCTGGT
SmaI 1268 CCCGGG CCC^GGG
2756 CCCGGG
Tru9I 591 TTAA T^TAA
1560 TTAA
Van91I 2335 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2637 CCAAGTTGTGG
XhoI 351 CTCGAG C^TCGAG
3330 CTCGAG
XmaCI 1268 CCCGGG C^CCGGG
2756 CCCGGG
XmaI 1268 CCCGGG C^CCGGG
2756 CCCGGG
XmnI 1294 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1880 GAAGGCGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI ApoI AspHI AvaI AvaII
BanII BbsI BbvI BcnI BglI
BmyI BpmI BpuAI BsaHI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII