################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AatII AccII AclI AflII Aor51HI AscI AseI AsnI BbeI BcgI BfrI BsiWI Bsp1407I BspHI BspLU11I BspMI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I CpoI EagI EclXI Eco47III Eco52I EcoO65I EcoT22I FseI HaeII HpaI KasI KspI MfeI MluI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SbfI ScaI SfiI SfoI SfuI SmaI SnaBI SphI Sse8387I SspBI SspI StuI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 2255 GGTACC G^GTACC AccIII 3029 TCCGGA T^CCGGA AflIII 3001 ACGTGT A^C[GA][CT]GT AgeI 1620 ACCGGT A^CCGGT AhdI 1596 GACAAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 19 GGGCCC GGGCC^C Asp718I 2255 GGTACC G^GTACC AspEI 1596 GACAAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1775 TGCGCA TGC^GCA AvrII 2087 CCTAGG C^CTAGG BamHI 3642 GGATCC G^GATCC BbrPI 3000 CACGTG CAC^GTG BbvCI 3528 GCTGAGG CCTCAGC(-5/-2) BglI 3856 GCCTGGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 8 AGATCT A^GATCT BlnI 2087 CCTAGG C^CTAGG BsaAI 3000 CACGTG [CT]AC^GT[GA] BsaBI 2118 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 3029 TCCGGA T^CCGGA BsiEI 1618 CGACCG CG[GA][CT]^CG BsmI 2769 GCATTC GAATGC(1/-1) BspDI 2106 ATCGAT AT^CGAT BspEI 3029 TCCGGA T^CCGGA BsrBI 1017 GAGCGG CCGCTC(-3/-3) BstAPI 1242 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC ClaI 2106 ATCGAT AT^CGAT DraI 3242 TTTAAA TTT^AAA DrdI 3717 GACCGCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 1596 GACAAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 2364 GAATTC G^AATTC FauI 3416 GCGGG CCCGC(4/6) FspI 1775 TGCGCA TGC^GCA KpnI 2255 GGTACC GGTAC^C MamI 2118 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 3029 TCCGGA T^CCGGA NcoI 3010 CCATGG C^CATGG PinAI 1620 ACCGGT A^CCGGT PmaCI 3000 CACGTG CAC^GTG PmlI 3000 CACGTG CAC^GTG SalI 348 GTCGAC G^TCGAC SexAI 945 ACCAGGT A^CC[AT]GGT SgrAI 2352 CGCCGGTG C[GA]^CCGG[CT]G SpeI 3648 ACTAGT A^CTAGT ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### AcyI 195 GGCGTC G[GA]^CG[CT]C 3594 GACGCC AfaI 2256 GTAC GT^AC 3047 GTAC Alw44I 2976 GTGCAC G^TGCAC 3005 GTGCAC AocI 149 CCTGAGG CC^T[ACGT]AGG 2232 CCTCAGG ApaLI 2976 GTGCAC G^TGCAC 3005 GTGCAC AspI 1974 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2009 GACTGAGTC AvaI 2412 CTCGAG C^[CT]CG[GA]G 2946 CTCGAG BciVI 941 GGATAC GTATCC(6/5) 1985 GGATAC BlpI 636 GCTGAGC GC^T[ACGT]AGC 2670 GCTCAGC Bpu1102I 636 GCTGAGC GC^T[ACGT]AGC 2670 GCTCAGC BsaHI 195 GGCGTC G[GA]^CG[CT]C 3594 GACGCC BsaI 213 GAGACC GGTCTC(1/5) 829 GAGACC BsgI 2357 GTGCAG GTGCAG(16/14) 3223 GTGCAG BsmBI 1164 GAGACG CGTCTC(1/5) 3125 GAGACG BsoBI 2412 CTCGAG C^[CT]CG[GA]G 2946 CTCGAG BsrDI 438 GCAATG GCAATG(2/0) 2515 GCAATG BssSI 1359 CACGAG CACGAG(-5/-1) 3171 CACGAG Bsu36I 149 CCTGAGG CC^T[ACGT]AGG 2232 CCTCAGG CelII 636 GCTGAGC GC^T[ACGT]AGC 2670 GCTCAGC CfoI 1776 GCGC GCG^C 3585 GCGC DsaI 3010 CCATGG C^C[GA][CT]GG 3765 CCACGG Eco81I 149 CCTGAGG CC^T[ACGT]AGG 2232 CCTCAGG EcoRV 1814 GATATC GAT^ATC 2103 GATATC HhaI 1776 GCGC GCG^C 3585 GCGC Hin1I 195 GGCGTC G[GA]^CG[CT]C 3594 GACGCC HinP1I 1776 GCGC G^CGC 3585 GCGC HindIII 279 AAGCTT A^AGCTT 3136 AAGCTT MaeII 496 ACGT A^CGT 3001 ACGT NspI 679 GCATGT [GA]CATG^[CT] 1705 ACATGC PaeR7I 2412 CTCGAG C^TCGAG 2946 CTCGAG PflFI 1974 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2009 GACTGAGTC RsaI 2256 GTAC GT^AC 3047 GTAC SacI 2055 GAGCTC GAGCT^C 2742 GAGCTC SapI 513 GAAGAGC GCTCTTC(1/4) 1449 GAAGAGC Tth111I 1974 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2009 GACTGAGTC XbaI 1094 TCTAGA T^CTAGA 1880 TCTAGA XcmI 1564 CCAGCAGCACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3748 CCAGTCAGAGAGTGG XhoI 2412 CTCGAG C^TCGAG 2946 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 104 enzymes) # ######################################### AccI AciI AcsI AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BalI BanI BanII BbsI BbvI BclI BcnI BfaI BmyI BpmI BpuAI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr10I Cfr13I DdeI DpnII DraII DraIII EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HapII HgaI HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI