##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AccII AclI AflII Aor51HI
AscI AseI AsnI BbeI BcgI
BfrI BsiWI Bsp1407I BspHI BspLU11I
BspMI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I CpoI
EagI EclXI Eco47III Eco52I EcoO65I
EcoT22I FseI HaeII HpaI KasI
KspI MfeI MluI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SbfI ScaI
SfiI SfoI SfuI SmaI SnaBI
SphI Sse8387I SspBI SspI StuI
SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 2255 GGTACC G^GTACC
AccIII 3029 TCCGGA T^CCGGA
AflIII 3001 ACGTGT A^C[GA][CT]GT
AgeI 1620 ACCGGT A^CCGGT
AhdI 1596 GACAAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 19 GGGCCC GGGCC^C
Asp718I 2255 GGTACC G^GTACC
AspEI 1596 GACAAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1775 TGCGCA TGC^GCA
AvrII 2087 CCTAGG C^CTAGG
BamHI 3642 GGATCC G^GATCC
BbrPI 3000 CACGTG CAC^GTG
BbvCI 3528 GCTGAGG CCTCAGC(-5/-2)
BglI 3856 GCCTGGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 8 AGATCT A^GATCT
BlnI 2087 CCTAGG C^CTAGG
BsaAI 3000 CACGTG [CT]AC^GT[GA]
BsaBI 2118 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3029 TCCGGA T^CCGGA
BsiEI 1618 CGACCG CG[GA][CT]^CG
BsmI 2769 GCATTC GAATGC(1/-1)
BspDI 2106 ATCGAT AT^CGAT
BspEI 3029 TCCGGA T^CCGGA
BsrBI 1017 GAGCGG CCGCTC(-3/-3)
BstAPI 1242 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 2106 ATCGAT AT^CGAT
DraI 3242 TTTAAA TTT^AAA
DrdI 3717 GACCGCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1596 GACAAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2364 GAATTC G^AATTC
FauI 3416 GCGGG CCCGC(4/6)
FspI 1775 TGCGCA TGC^GCA
KpnI 2255 GGTACC GGTAC^C
MamI 2118 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3029 TCCGGA T^CCGGA
NcoI 3010 CCATGG C^CATGG
PinAI 1620 ACCGGT A^CCGGT
PmaCI 3000 CACGTG CAC^GTG
PmlI 3000 CACGTG CAC^GTG
SalI 348 GTCGAC G^TCGAC
SexAI 945 ACCAGGT A^CC[AT]GGT
SgrAI 2352 CGCCGGTG C[GA]^CCGG[CT]G
SpeI 3648 ACTAGT A^CTAGT
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AcyI 195 GGCGTC G[GA]^CG[CT]C
3594 GACGCC
AfaI 2256 GTAC GT^AC
3047 GTAC
Alw44I 2976 GTGCAC G^TGCAC
3005 GTGCAC
AocI 149 CCTGAGG CC^T[ACGT]AGG
2232 CCTCAGG
ApaLI 2976 GTGCAC G^TGCAC
3005 GTGCAC
AspI 1974 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2009 GACTGAGTC
AvaI 2412 CTCGAG C^[CT]CG[GA]G
2946 CTCGAG
BciVI 941 GGATAC GTATCC(6/5)
1985 GGATAC
BlpI 636 GCTGAGC GC^T[ACGT]AGC
2670 GCTCAGC
Bpu1102I 636 GCTGAGC GC^T[ACGT]AGC
2670 GCTCAGC
BsaHI 195 GGCGTC G[GA]^CG[CT]C
3594 GACGCC
BsaI 213 GAGACC GGTCTC(1/5)
829 GAGACC
BsgI 2357 GTGCAG GTGCAG(16/14)
3223 GTGCAG
BsmBI 1164 GAGACG CGTCTC(1/5)
3125 GAGACG
BsoBI 2412 CTCGAG C^[CT]CG[GA]G
2946 CTCGAG
BsrDI 438 GCAATG GCAATG(2/0)
2515 GCAATG
BssSI 1359 CACGAG CACGAG(-5/-1)
3171 CACGAG
Bsu36I 149 CCTGAGG CC^T[ACGT]AGG
2232 CCTCAGG
CelII 636 GCTGAGC GC^T[ACGT]AGC
2670 GCTCAGC
CfoI 1776 GCGC GCG^C
3585 GCGC
DsaI 3010 CCATGG C^C[GA][CT]GG
3765 CCACGG
Eco81I 149 CCTGAGG CC^T[ACGT]AGG
2232 CCTCAGG
EcoRV 1814 GATATC GAT^ATC
2103 GATATC
HhaI 1776 GCGC GCG^C
3585 GCGC
Hin1I 195 GGCGTC G[GA]^CG[CT]C
3594 GACGCC
HinP1I 1776 GCGC G^CGC
3585 GCGC
HindIII 279 AAGCTT A^AGCTT
3136 AAGCTT
MaeII 496 ACGT A^CGT
3001 ACGT
NspI 679 GCATGT [GA]CATG^[CT]
1705 ACATGC
PaeR7I 2412 CTCGAG C^TCGAG
2946 CTCGAG
PflFI 1974 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2009 GACTGAGTC
RsaI 2256 GTAC GT^AC
3047 GTAC
SacI 2055 GAGCTC GAGCT^C
2742 GAGCTC
SapI 513 GAAGAGC GCTCTTC(1/4)
1449 GAAGAGC
Tth111I 1974 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2009 GACTGAGTC
XbaI 1094 TCTAGA T^CTAGA
1880 TCTAGA
XcmI 1564 CCAGCAGCACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3748 CCAGTCAGAGAGTGG
XhoI 2412 CTCGAG C^TCGAG
2946 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccI AciI AcsI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BalI BanI BanII BbsI BbvI
BclI BcnI BfaI BmyI BpmI
BpuAI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr10I Cfr13I
DdeI DpnII DraII DraIII EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FbaI Fnu4HI FokI HaeIII HapII
HgaI HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI