##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BamHI BbeI BbrPI
BcgI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspMI BsrGI
BssHII BssSI BstBI BstEII BstPI
BstUI CelII CfoI ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoT22I
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HpaI KasI
KpnI KspI MfeI MluI MroI
MunI MvnI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 1982 ACATGT A^C[GA][CT]GT
Asp700I 3412 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1964 TGGCCA TGG^CCA
BbvCI 1055 CCTCAGC CCTCAGC(-5/-2)
BciVI 284 GGATAC GTATCC(6/5)
BclI 218 TGATCA T^GATCA
BspLU11I 1982 ACATGT A^CATGT
BsrFI 3190 GCCGGC [GA]^CCGG[CT]
Bst1107I 3225 GTATAC GTA^TAC
BstAPI 2131 GCACAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 3225 GTATAC GTA^TAC
Cfr10I 3190 GCCGGC [GA]^CCGG[CT]
DsaI 3317 CCATGG C^C[GA][CT]GG
EcoNI 3447 CCTTCCGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 218 TGATCA T^GATCA
MaeII 2594 ACGT A^CGT
MluNI 1964 TGGCCA TGG^CCA
MscI 1964 TGGCCA TGG^CCA
NaeI 3190 GCCGGC GCC^GGC
NcoI 3317 CCATGG C^CATGG
NgoMIV 3190 GCCGGC G^CCGGC
PvuII 3238 CAGCTG CAG^CTG
SapI 2764 GAAGAGC GCTCTTC(1/4)
ScaI 3473 AGTACT AGT^ACT
SexAI 3124 ACCAGGT A^CC[AT]GGT
SmlI 2964 CTTGAG C^T[CT][GA]AG
SspI 753 AATATT AAT^ATT
StuI 3444 AGGCCT AGG^CCT
XmnI 3412 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 788 GTAC GT^AC
3474 GTAC
BanI 2490 GGCACC G^G[CT][GA]CC
2538 GGCACC
BsaBI 1139 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3003 GATAGACATC
BsrBI 1579 CCGCTC CCGCTC(-3/-3)
1714 GAGCGG
DrdI 313 GACTCTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1472 GACTCTCAGGTC
EaeI 1964 TGGCCA [CT]^GGCC[GA]
3188 TGGCCG
HindIII 1247 AAGCTT A^AGCTT
2957 AAGCTT
MamI 1139 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3003 GATAGACATC
MspA1I 629 CAGCGG C[AC]G^C[GT]G
3238 CAGCTG
PpuMI 1479 AGGTCCT [GA]G^G[AT]CC[CT]
2530 AGGTCCC
RsaI 788 GTAC GT^AC
3474 GTAC
SmaI 2151 CCCGGG CCC^GGG
2780 CCCGGG
TaqI 1768 TCGA T^CGA
3432 TCGA
TthHB8I 1768 TCGA T^CGA
3432 TCGA
XbaI 1939 TCTAGA T^CTAGA
2308 TCTAGA
XmaCI 2151 CCCGGG C^CCGGG
2780 CCCGGG
XmaI 2151 CCCGGG C^CCGGG
2780 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccI AciI AluI AlwI AlwNI
AocI AspHI AspI AvaI AvaII
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII DraII EarI Eco81I
EcoO109I EcoRII EcoRV EcoT14I Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PshAI
PstI RcaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I Van91I XcmI XhoII