################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## AatII AccI AccII AccIII AclI AflII AhdI Alw44I ApaLI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BamHI BbrPI BcgI BfrI BglII BlnI BsaAI BsaBI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BsrDI BsrGI BssHII Bst1107I BstBI BstUI BstZ17I ClaI CpoI DraI EagI Eam1105I EclXI Eco52I EcoRV EcoT22I FspI HindIII HpaI KspI MamI MfeI MluI MroI MseI MunI MvnI NcoI NdeI NheI NotI NruI NsiI NspV PacI PflFI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfuI SnaBI SpeI Sse8387I SspBI SspI SwaI Tru9I Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 1178 GGTACC G^GTACC AflIII 926 ACATGT A^C[GA][CT]GT AgeI 1707 ACCGGT A^CCGGT AocI 1312 CCTGAGG CC^T[ACGT]AGG Aor51HI 239 AGCGCT AGC^GCT Asp718I 1178 GGTACC G^GTACC BbeI 1617 GGCGCC GGCGC^C BfaI 2279 CTAG C^TAG BlpI 699 GCTGAGC GC^T[ACGT]AGC Bpu1102I 699 GCTGAGC GC^T[ACGT]AGC BsaI 1426 GAGACC GGTCTC(1/5) BsiEI 207 CGACCG CG[GA][CT]^CG BsmBI 919 GAGACG CGTCTC(1/5) BspLU11I 926 ACATGT A^CATGT BstAPI 156 GCAGGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 117 GGTGACC G^GT[ACGT]ACC BstPI 117 GGTGACC G^GT[ACGT]ACC Bsu36I 1312 CCTGAGG CC^T[ACGT]AGG CelII 699 GCTGAGC GC^T[ACGT]AGC DraIII 1629 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1011 GACCCCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 239 AGCGCT AGC^GCT Eco81I 1312 CCTGAGG CC^T[ACGT]AGG EcoO65I 117 GGTGACC G^GT[ACGT]ACC EcoRI 322 GAATTC G^AATTC FseI 1396 GGCCGGCC GGCCGG^CC KasI 1617 GGCGCC G^GCGCC KpnI 1178 GGTACC GGTAC^C MaeI 2279 CTAG C^TAG MaeII 982 ACGT A^CGT NaeI 1397 GCCGGC GCC^GGC NarI 1617 GGCGCC GG^CGCC NgoMIV 1397 GCCGGC G^CCGGC PaeR7I 1084 CTCGAG C^TCGAG PinAI 1707 ACCGGT A^CCGGT PpuMI 1605 GGGTCCC [GA]G^G[AT]CC[CT] SexAI 313 ACCTGGT A^CC[AT]GGT SfiI 832 GGCCCGGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 1617 GGCGCC GGC^GCC SgrAI 375 CGCCGGTG C[GA]^CCGG[CT]G SmaI 1738 CCCGGG CCC^GGG SphI 1553 GCATGC GCATG^C XhoI 1084 CTCGAG C^TCGAG XmaCI 1738 CCCGGG C^CCGGG XmaI 1738 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AcsI 322 GAATTC [GA]^AATT[CT] 1405 GAATTT AfaI 930 GTAC GT^AC 1179 GTAC AlwNI 269 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG 346 CAGGATCTG ApaI 855 GGGCCC GGGCC^C 1089 GGGCCC ApoI 322 GAATTC [GA]^AATT[CT] 1405 GAATTT AvaII 149 GGACC G^G[AT]CC 1606 GGTCC BbsI 964 GAAGAC GAAGAC(2/6) 1020 GTCTTC BclI 353 TGATCA T^GATCA 821 TGATCA BpuAI 964 GAAGAC GAAGAC(2/6) 1020 GTCTTC BsaWI 146 ACCGGA [AT]^CCGG[AT] 1707 ACCGGT BsgI 1276 CTGCAC GTGCAG(16/14) 1456 CTGCAC BsmI 1329 GAATGC GAATGC(1/-1) 82 GCATTC BspMI 183 ACCTGC ACCTGC(4/8) 1040 ACCTGC BsrBI 562 CCGCTC CCGCTC(-3/-3) 1095 GAGCGG BssSI 1076 CTCGTG CACGAG(-5/-1) 1934 CTCGTG DsaI 2039 CCACGG C^C[GA][CT]GG 2114 CCACGG EarI 490 CTCTTC CTCTTC(1/4) 585 GAAGAG FauI 402 CCCGC CCCGC(4/6) 1907 CCCGC FbaI 353 TGATCA T^GATCA 821 TGATCA HgaI 259 GACGC GACGC(5/10) 2187 GACGC HincII 517 GTCAAC GT[CT]^[GA]AC 2025 GTCAAC HindII 517 GTCAAC GT[CT]^[GA]AC 2025 GTCAAC Ksp632I 490 CTCTTC CTCTTC(1/4) 585 GAAGAG NspI 926 ACATGT [GA]CATG^[CT] 1553 GCATGC PflMI 1628 CCACCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1692 CCAAGGTATGG PstI 1960 CTGCAG CTGCA^G 1990 CTGCAG RsaI 930 GTAC GT^AC 1179 GTAC SacI 1251 GAGCTC GAGCT^C 1924 GAGCTC TfiI 658 GAATC G^A[AT]TC 893 GAATC Tsp509I 323 AATT ^AATT 1406 AATT Van91I 1628 CCACCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1692 CCAAGGTATGG XcmI 464 CCATCTGCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1688 CCAGCCAAGGTATGG ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AciI AcyI AluI AlwI AspHI AvaI BalI BanI BanII BbvCI BbvI BciVI BcnI BglI BmyI BpmI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TseI Tsp45I TspRI TthHB8I XhoII