##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AhdI Alw44I ApaLI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BamHI BbrPI BcgI
BfrI BglII BlnI BsaAI BsaBI
BseAI BsiWI Bsp1407I BspDI BspEI
BspHI BsrDI BsrGI BssHII Bst1107I
BstBI BstUI BstZ17I ClaI CpoI
DraI EagI Eam1105I EclXI Eco52I
EcoRV EcoT22I FspI HindIII HpaI
KspI MamI MfeI MluI MroI
MseI MunI MvnI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PflFI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SfuI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tru9I Tth111I
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 1178 GGTACC G^GTACC
AflIII 926 ACATGT A^C[GA][CT]GT
AgeI 1707 ACCGGT A^CCGGT
AocI 1312 CCTGAGG CC^T[ACGT]AGG
Aor51HI 239 AGCGCT AGC^GCT
Asp718I 1178 GGTACC G^GTACC
BbeI 1617 GGCGCC GGCGC^C
BfaI 2279 CTAG C^TAG
BlpI 699 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 699 GCTGAGC GC^T[ACGT]AGC
BsaI 1426 GAGACC GGTCTC(1/5)
BsiEI 207 CGACCG CG[GA][CT]^CG
BsmBI 919 GAGACG CGTCTC(1/5)
BspLU11I 926 ACATGT A^CATGT
BstAPI 156 GCAGGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 117 GGTGACC G^GT[ACGT]ACC
BstPI 117 GGTGACC G^GT[ACGT]ACC
Bsu36I 1312 CCTGAGG CC^T[ACGT]AGG
CelII 699 GCTGAGC GC^T[ACGT]AGC
DraIII 1629 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1011 GACCCCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 239 AGCGCT AGC^GCT
Eco81I 1312 CCTGAGG CC^T[ACGT]AGG
EcoO65I 117 GGTGACC G^GT[ACGT]ACC
EcoRI 322 GAATTC G^AATTC
FseI 1396 GGCCGGCC GGCCGG^CC
KasI 1617 GGCGCC G^GCGCC
KpnI 1178 GGTACC GGTAC^C
MaeI 2279 CTAG C^TAG
MaeII 982 ACGT A^CGT
NaeI 1397 GCCGGC GCC^GGC
NarI 1617 GGCGCC GG^CGCC
NgoMIV 1397 GCCGGC G^CCGGC
PaeR7I 1084 CTCGAG C^TCGAG
PinAI 1707 ACCGGT A^CCGGT
PpuMI 1605 GGGTCCC [GA]G^G[AT]CC[CT]
SexAI 313 ACCTGGT A^CC[AT]GGT
SfiI 832 GGCCCGGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1617 GGCGCC GGC^GCC
SgrAI 375 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 1738 CCCGGG CCC^GGG
SphI 1553 GCATGC GCATG^C
XhoI 1084 CTCGAG C^TCGAG
XmaCI 1738 CCCGGG C^CCGGG
XmaI 1738 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcsI 322 GAATTC [GA]^AATT[CT]
1405 GAATTT
AfaI 930 GTAC GT^AC
1179 GTAC
AlwNI 269 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
346 CAGGATCTG
ApaI 855 GGGCCC GGGCC^C
1089 GGGCCC
ApoI 322 GAATTC [GA]^AATT[CT]
1405 GAATTT
AvaII 149 GGACC G^G[AT]CC
1606 GGTCC
BbsI 964 GAAGAC GAAGAC(2/6)
1020 GTCTTC
BclI 353 TGATCA T^GATCA
821 TGATCA
BpuAI 964 GAAGAC GAAGAC(2/6)
1020 GTCTTC
BsaWI 146 ACCGGA [AT]^CCGG[AT]
1707 ACCGGT
BsgI 1276 CTGCAC GTGCAG(16/14)
1456 CTGCAC
BsmI 1329 GAATGC GAATGC(1/-1)
82 GCATTC
BspMI 183 ACCTGC ACCTGC(4/8)
1040 ACCTGC
BsrBI 562 CCGCTC CCGCTC(-3/-3)
1095 GAGCGG
BssSI 1076 CTCGTG CACGAG(-5/-1)
1934 CTCGTG
DsaI 2039 CCACGG C^C[GA][CT]GG
2114 CCACGG
EarI 490 CTCTTC CTCTTC(1/4)
585 GAAGAG
FauI 402 CCCGC CCCGC(4/6)
1907 CCCGC
FbaI 353 TGATCA T^GATCA
821 TGATCA
HgaI 259 GACGC GACGC(5/10)
2187 GACGC
HincII 517 GTCAAC GT[CT]^[GA]AC
2025 GTCAAC
HindII 517 GTCAAC GT[CT]^[GA]AC
2025 GTCAAC
Ksp632I 490 CTCTTC CTCTTC(1/4)
585 GAAGAG
NspI 926 ACATGT [GA]CATG^[CT]
1553 GCATGC
PflMI 1628 CCACCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1692 CCAAGGTATGG
PstI 1960 CTGCAG CTGCA^G
1990 CTGCAG
RsaI 930 GTAC GT^AC
1179 GTAC
SacI 1251 GAGCTC GAGCT^C
1924 GAGCTC
TfiI 658 GAATC G^A[AT]TC
893 GAATC
Tsp509I 323 AATT ^AATT
1406 AATT
Van91I 1628 CCACCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1692 CCAAGGTATGG
XcmI 464 CCATCTGCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1688 CCAGCCAAGGTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcyI AluI AlwI AspHI
AvaI BalI BanI BanII BbvCI
BbvI BciVI BcnI BglI BmyI
BpmI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TseI
Tsp45I TspRI TthHB8I XhoII