##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BbrPI BbvCI BcgI
BfrI BlnI BlpI Bpu1102I BsaAI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspHI BsrGI BssHII Bst1107I
BstAPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRV FseI FspI HindIII HpaI
KpnI KspI MaeII MfeI MluI
MroI MunI NaeI NdeI NgoMIV
NheI NotI NruI PacI PaeR7I
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SfiI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 2041 GTCTAC GT^[AC][GT]AC
AflIII 572 ACATGT A^C[GA][CT]GT
AlwNI 611 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 702 GGGCCC GGGCC^C
AspHI 606 GAGCAC G[AT]GC[AT]^C
AspI 2188 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 825 GGATCC G^GATCC
BanI 628 GGCGCC G^G[CT][GA]CC
BbeI 628 GGCGCC GGCGC^C
BciVI 376 GGATAC GTATCC(6/5)
BglII 1383 AGATCT A^GATCT
BsaBI 826 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 673 GTGCAG GTGCAG(16/14)
BsiHKAI 606 GAGCAC G[AT]GC[AT]^C
BsmBI 535 GAGACG CGTCTC(1/5)
BsmI 1418 GAATGC GAATGC(1/-1)
BspLU11I 572 ACATGT A^CATGT
BspMI 8 ACCTGC ACCTGC(4/8)
BssSI 252 CTCGTG CACGAG(-5/-1)
BstBI 1259 TTCGAA TT^CGAA
BstEII 921 GGTAACC G^GT[ACGT]ACC
BstPI 921 GGTAACC G^GT[ACGT]ACC
EcoO65I 921 GGTAACC G^GT[ACGT]ACC
EcoRI 909 GAATTC G^AATTC
EcoT22I 1420 ATGCAT ATGCA^T
HincII 121 GTTGAC GT[CT]^[GA]AC
HindII 121 GTTGAC GT[CT]^[GA]AC
KasI 628 GGCGCC G^GCGCC
MamI 826 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 2431 CAGCTG C[AC]G^C[GT]G
NarI 628 GGCGCC GG^CGCC
NsiI 1420 ATGCAT ATGCA^T
NspI 572 ACATGT [GA]CATG^[CT]
NspV 1259 TTCGAA TT^CGAA
PflFI 2188 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1665 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1650 GACCACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 2431 CAGCTG CAG^CTG
SapI 802 GAAGAGC GCTCTTC(1/4)
SexAI 1796 ACCAGGT A^CC[AT]GGT
SfoI 628 GGCGCC GGC^GCC
SfuI 1259 TTCGAA TT^CGAA
SmaI 2315 CCCGGG CCC^GGG
SspI 1032 AATATT AAT^ATT
Tth111I 2188 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1665 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 2315 CCCGGG C^CCGGG
XmaI 2315 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 133 CGCG CG^CG
1188 CGCG
AgeI 363 ACCGGT A^CCGGT
1676 ACCGGT
Asp700I 165 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2324 GAACACTTTC
AvaI 71 CTCGGG C^[CT]CG[GA]G
2315 CCCGGG
BalI 554 TGGCCA TGG^CCA
1662 TGGCCA
BanII 702 GGGCCC G[GA]GC[CT]^C
2312 GAGCCC
BbsI 2200 GAAGAC GAAGAC(2/6)
2469 GTCTTC
BglI 1085 GCCCCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1339 GCCCCCTTGGC
BpmI 542 CTCCAG CTGGAG(16/14)
1972 CTCCAG
BpuAI 2200 GAAGAC GAAGAC(2/6)
2469 GTCTTC
BsoBI 71 CTCGGG C^[CT]CG[GA]G
2315 CCCGGG
BsrBI 2296 CCGCTC CCGCTC(-3/-3)
654 GAGCGG
BsrFI 363 ACCGGT [GA]^CCGG[CT]
1676 ACCGGT
BstUI 133 CGCG CG^CG
1188 CGCG
BstXI 361 CCACCGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2351 CCAACTTTGTGG
Cfr10I 363 ACCGGT [GA]^CCGG[CT]
1676 ACCGGT
DsaI 623 CCATGG C^C[GA][CT]GG
2105 CCATGG
FauI 2295 CCCGC CCCGC(4/6)
656 GCGGG
HaeII 203 AGCGCC [GA]GCGC^[CT]
628 GGCGCC
MluNI 554 TGGCCA TGG^CCA
1662 TGGCCA
MscI 554 TGGCCA TGG^CCA
1662 TGGCCA
MvnI 133 CGCG CG^CG
1188 CGCG
NcoI 623 CCATGG C^CATGG
2105 CCATGG
PinAI 363 ACCGGT A^CCGGT
1676 ACCGGT
TaqI 83 TCGA T^CGA
1260 TCGA
TthHB8I 83 TCGA T^CGA
1260 TCGA
XbaI 264 TCTAGA T^CTAGA
1713 TCTAGA
XcmI 217 CCAGAAGCCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1843 CCACTGGGATCGTGG
XmnI 165 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2324 GAACACTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AvaII BbvI BclI
BcnI BfaI BmyI BsaHI BsaI
BsaJI BsaWI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FbaI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII