##################################
# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Alw44I
AocI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglII BlnI BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DrdI DsaI EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
TaqI TthHB8I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 301 GAATTT [GA]^AATT[CT]
AflIII 1274 ACGTGT A^C[GA][CT]GT
Aor51HI 884 AGCGCT AGC^GCT
ApoI 301 GAATTT [GA]^AATT[CT]
BamHI 1385 GGATCC G^GATCC
BanI 714 GGTGCC G^G[CT][GA]CC
BclI 1164 TGATCA T^GATCA
BlpI 1846 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1846 GCTCAGC GC^T[ACGT]AGC
BsmBI 963 GAGACG CGTCTC(1/5)
BsmI 1243 GAATGC GAATGC(1/-1)
CelII 1846 GCTCAGC GC^T[ACGT]AGC
DraIII 1080 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 884 AGCGCT AGC^GCT
FbaI 1164 TGATCA T^GATCA
HindIII 826 AAGCTT A^AGCTT
PflMI 1764 CCAGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1722 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 1145 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1362 GAGCTC GAGCT^C
ScaI 853 AGTACT AGT^ACT
SexAI 386 ACCTGGT A^CC[AT]GGT
SspI 1178 AATATT AAT^ATT
Van91I 1764 CCAGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1943 CCAAGGAAACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 459 GACGCC G[GA]^CG[CT]C
965 GACGCC
Asp700I 1344 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1485 GAAGCCCTTC
AspI 637 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
922 GACAGAGTC
BglI 48 GCCTTGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
184 GCCTTGCTGGC
BsaHI 459 GACGCC G[GA]^CG[CT]C
965 GACGCC
BsmAI 963 GAGAC GTCTC(1/5)
1458 GAGAC
BsrBI 1844 CCGCTC CCGCTC(-3/-3)
1063 GAGCGG
BstYI 350 AGATCC [GA]^GATC[CT]
1385 GGATCC
DraI 625 TTTAAA TTT^AAA
931 TTTAAA
DraII 1722 AGGTCCC [GA]G^G[ACGT]CC[CT]
1829 AGGGCCT
EarI 5 CTCTTC CTCTTC(1/4)
229 GAAGAG
EcoNI 387 CCTGGTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2036 CCTCCACTAGG
EcoO109I 1722 AGGTCCC [GA]G^G[ACGT]CC[CT]
1829 AGGGCCT
EcoT14I 746 CCAAGG C^C[AT][AT]GG
1943 CCAAGG
HgaI 459 GACGC GACGC(5/10)
965 GACGC
Hin1I 459 GACGCC G[GA]^CG[CT]C
965 GACGCC
Ksp632I 5 CTCTTC CTCTTC(1/4)
229 GAAGAG
MaeII 1274 ACGT A^CGT
1657 ACGT
MflI 350 AGATCC [GA]^GATC[CT]
1385 GGATCC
MslI 325 CATGCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1210 CATTATCGTG
PflFI 637 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
922 GACAGAGTC
StyI 746 CCAAGG C^C[AT][AT]GG
1943 CCAAGG
Tth111I 637 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
922 GACAGAGTC
XhoII 350 AGATCC [GA]^GATC[CT]
1385 GGATCC
XmnI 1344 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1485 GAAGCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BalI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EcoRII Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MluNI
MnlI MscI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI