##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BciVI BfrI BlnI BsaBI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BspLU11I BssHII Bst1107I BstAPI BstBI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoRI EcoRV EcoT22I
FseI FspI HpaI KpnI KspI
MamI MfeI MluI MroI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SspI SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccII 1648 CGCG CG^CG
AcyI 2007 GGCGCC G[GA]^CG[CT]C
Aor51HI 1269 AGCGCT AGC^GCT
ApaI 1575 GGGCCC GGGCC^C
BamHI 2349 GGATCC G^GATCC
BbeI 2007 GGCGCC GGCGC^C
BbrPI 1106 CACGTG CAC^GTG
BbvCI 1395 CCTCAGC CCTCAGC(-5/-2)
BcgI 790 CGAGGTGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 456 TGATCA T^GATCA
BglII 1042 AGATCT A^GATCT
BsaAI 1106 CACGTG [CT]AC^GT[GA]
BsaHI 2007 GGCGCC G[GA]^CG[CT]C
BsaWI 741 ACCGGA [AT]^CCGG[AT]
BspHI 390 TCATGA T^CATGA
BsrDI 1741 CATTGC GCAATG(2/0)
BsrFI 974 GCCGGC [GA]^CCGG[CT]
BstEII 2141 GGTGACC G^GT[ACGT]ACC
BstPI 2141 GGTGACC G^GT[ACGT]ACC
BstUI 1648 CGCG CG^CG
Cfr10I 974 GCCGGC [GA]^CCGG[CT]
Eco47III 1269 AGCGCT AGC^GCT
EcoO65I 2141 GGTGACC G^GT[ACGT]ACC
FbaI 456 TGATCA T^GATCA
HgaI 483 GCGTC GACGC(5/10)
Hin1I 2007 GGCGCC G[GA]^CG[CT]C
HindIII 1892 AAGCTT A^AGCTT
KasI 2007 GGCGCC G^GCGCC
MaeII 1107 ACGT A^CGT
MseI 891 TTAA T^TAA
MvnI 1648 CGCG CG^CG
NaeI 974 GCCGGC GCC^GGC
NarI 2007 GGCGCC GG^CGCC
NcoI 927 CCATGG C^CATGG
NgoMIV 974 GCCGGC G^CCGGC
NspI 796 GCATGC [GA]CATG^[CT]
PmaCI 1106 CACGTG CAC^GTG
PmlI 1106 CACGTG CAC^GTG
PpuMI 1862 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 390 TCATGA T^CATGA
SapI 613 GCTCTTC GCTCTTC(1/4)
ScaI 2742 AGTACT AGT^ACT
SfoI 2007 GGCGCC GGC^GCC
SphI 796 GCATGC GCATG^C
StuI 822 AGGCCT AGG^CCT
TaqI 650 TCGA T^CGA
Tru9I 891 TTAA T^TAA
TthHB8I 650 TCGA T^CGA
XbaI 2218 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 485 GTCTAC GT^[AC][GT]AC
2282 GTCTAC
AlwNI 15 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
2410 CAGTTACTG
Asp700I 424 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
820 GAAGGCCTTC
AspHI 149 GAGCAC G[AT]GC[AT]^C
1109 GTGCTC
AspI 1660 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2225 GACCCCGTC
AvaI 1169 CCCGAG C^[CT]CG[GA]G
2051 CCCGAG
BlpI 146 GCTGAGC GC^T[ACGT]AGC
1630 GCTGAGC
Bpu1102I 146 GCTGAGC GC^T[ACGT]AGC
1630 GCTGAGC
BsaI 2648 GAGACC GGTCTC(1/5)
1318 GGTCTC
BsgI 2828 CTGCAC GTGCAG(16/14)
1353 GTGCAG
BsiHKAI 149 GAGCAC G[AT]GC[AT]^C
1109 GTGCTC
BsmI 713 GAATGC GAATGC(1/-1)
2197 GAATGC
BsoBI 1169 CCCGAG C^[CT]CG[GA]G
2051 CCCGAG
Bsp1407I 670 TGTACA T^GTACA
2313 TGTACA
BsrGI 670 TGTACA T^GTACA
2313 TGTACA
BssSI 2666 CTCGTG CACGAG(-5/-1)
2688 CTCGTG
CelII 146 GCTGAGC GC^T[ACGT]AGC
1630 GCTGAGC
EcoNI 112 CCTCGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2622 CCTGGGCCAGG
EcoT14I 306 CCAAGG C^C[AT][AT]GG
927 CCATGG
HincII 2036 GTCAAC GT[CT]^[GA]AC
2811 GTTGAC
HindII 2036 GTCAAC GT[CT]^[GA]AC
2811 GTTGAC
PflFI 1660 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2225 GACCCCGTC
PflMI 295 CCAGCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
922 CCAGGCCATGG
SbfI 752 CCTGCAGG CCTGCA^GG
1579 CCTGCAGG
Sse8387I 752 CCTGCAGG CCTGCA^GG
1579 CCTGCAGG
SspBI 670 TGTACA T^GTACA
2313 TGTACA
StyI 306 CCAAGG C^C[AT][AT]GG
927 CCATGG
TfiI 2684 GATTC G^A[AT]TC
2693 GATTC
Tsp509I 956 AATT ^AATT
2293 AATT
Tth111I 1660 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2225 GACCCCGTC
Van91I 295 CCAGCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
922 CCAGGCCATGG
XmnI 424 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
820 GAAGGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrBI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TseI Tsp45I TspRI
XcmI XhoII