##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI Alw44I
AocI ApaLI AscI AseI AsnI
Asp718I AspI AviII AvrII BbeI
BbrPI BcnI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaWI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII EagI EclXI
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HindIII
HpaI KasI KpnI KspI MfeI
MluI MroI MunI MvnI NarI
NciI NdeI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AciI 776 GCGG CCGC(-3/-1)
AcsI 1269 AAATTC [GA]^AATT[CT]
AcyI 1689 GACGCC G[GA]^CG[CT]C
AfaI 552 GTAC GT^AC
Aor51HI 2049 AGCGCT AGC^GCT
ApoI 1269 AAATTC [GA]^AATT[CT]
AvaI 145 CCCGAG C^[CT]CG[GA]G
BamHI 1525 GGATCC G^GATCC
BbvCI 616 CCTCAGC CCTCAGC(-5/-2)
BcgI 147 CGAGGGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 104 GGATAC GTATCC(6/5)
BclI 530 TGATCA T^GATCA
BsaBI 531 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1689 GACGCC G[GA]^CG[CT]C
BsaI 62 GGTCTC GGTCTC(1/5)
BsmBI 586 CGTCTC CGTCTC(1/5)
BsoBI 145 CCCGAG C^[CT]CG[GA]G
BsrDI 454 GCAATG GCAATG(2/0)
BsrFI 772 GCCGGC [GA]^CCGG[CT]
BstAPI 179 GCAGAGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1525 GGATCC [GA]^GATC[CT]
Cfr10I 772 GCCGGC [GA]^CCGG[CT]
DrdI 1338 GACACAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2049 AGCGCT AGC^GCT
EcoNI 351 CCTTGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 530 TGATCA T^GATCA
HapII 773 CCGG C^CGG
HgaI 1689 GACGC GACGC(5/10)
Hin1I 1689 GACGCC G[GA]^CG[CT]C
HincII 2010 GTCAAC GT[CT]^[GA]AC
HindII 2010 GTCAAC GT[CT]^[GA]AC
HpaII 773 CCGG C^CGG
MaeII 573 ACGT A^CGT
MamI 531 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1525 GGATCC [GA]^GATC[CT]
MspA1I 1296 CAGCTG C[AC]G^C[GT]G
MspI 773 CCGG C^CGG
NaeI 772 GCCGGC GCC^GGC
NcoI 842 CCATGG C^CATGG
NgoMIV 772 GCCGGC G^CCGGC
NheI 95 GCTAGC G^CTAGC
NspI 474 ACATGC [GA]CATG^[CT]
PshAI 568 GACAAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1296 CAGCTG CAG^CTG
RsaI 552 GTAC GT^AC
Tsp45I 86 GTCAC ^GT[GC]AC
XhoII 1525 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Asp700I 1485 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1918 GAAACCCTTC
AspHI 1419 GAGCTC G[AT]GC[AT]^C
2039 GAGCTC
BanI 669 GGTGCC G^G[CT][GA]CC
2158 GGCACC
BglI 947 GCCTGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1752 GCCCCCCAGGC
BsiHKAI 1419 GAGCTC G[AT]GC[AT]^C
2039 GAGCTC
BsmI 1442 GAATGC GAATGC(1/-1)
522 GCATTC
BspMI 2161 ACCTGC ACCTGC(4/8)
2303 ACCTGC
CfoI 960 GCGC GCG^C
2050 GCGC
EarI 741 CTCTTC CTCTTC(1/4)
828 GAAGAG
HaeII 959 GGCGCT [GA]GCGC^[CT]
2049 AGCGCT
HhaI 960 GCGC GCG^C
2050 GCGC
HinP1I 960 GCGC G^CGC
2050 GCGC
Ksp632I 741 CTCTTC CTCTTC(1/4)
828 GAAGAG
MaeIII 40 GTAAC ^GT[ACGT]AC
86 GTCAC
SacI 1419 GAGCTC GAGCT^C
2039 GAGCTC
SbfI 170 CCTGCAGG CCTGCA^GG
948 CCTGCAGG
SmlI 605 CTCAAG C^T[CT][GA]AG
1037 CTCAAG
Sse8387I 170 CCTGCAGG CCTGCA^GG
948 CCTGCAGG
StuI 1182 AGGCCT AGG^CCT
2114 AGGCCT
TaqI 590 TCGA T^CGA
1385 TCGA
TthHB8I 590 TCGA T^CGA
1385 TCGA
XmnI 1485 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1918 GAAACCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AhdI AluI AlwI AlwNI ApaI
AspEI AvaII BalI BanII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
Eam1105I EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MboI MboII MluNI MnlI
MscI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI Tru9I
TseI Tsp509I TspRI Van91I XcmI