################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AflIII AgeI Alw44I AocI ApaLI AscI AseI AsnI Asp718I AspI AviII AvrII BbeI BbrPI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaWI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII EagI EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HindIII HpaI KasI KpnI KspI MfeI MluI MroI MunI MvnI NarI NciI NdeI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AciI 776 GCGG CCGC(-3/-1) AcsI 1269 AAATTC [GA]^AATT[CT] AcyI 1689 GACGCC G[GA]^CG[CT]C AfaI 552 GTAC GT^AC Aor51HI 2049 AGCGCT AGC^GCT ApoI 1269 AAATTC [GA]^AATT[CT] AvaI 145 CCCGAG C^[CT]CG[GA]G BamHI 1525 GGATCC G^GATCC BbvCI 616 CCTCAGC CCTCAGC(-5/-2) BcgI 147 CGAGGGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 104 GGATAC GTATCC(6/5) BclI 530 TGATCA T^GATCA BsaBI 531 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1689 GACGCC G[GA]^CG[CT]C BsaI 62 GGTCTC GGTCTC(1/5) BsmBI 586 CGTCTC CGTCTC(1/5) BsoBI 145 CCCGAG C^[CT]CG[GA]G BsrDI 454 GCAATG GCAATG(2/0) BsrFI 772 GCCGGC [GA]^CCGG[CT] BstAPI 179 GCAGAGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 1525 GGATCC [GA]^GATC[CT] Cfr10I 772 GCCGGC [GA]^CCGG[CT] DrdI 1338 GACACAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2049 AGCGCT AGC^GCT EcoNI 351 CCTTGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 530 TGATCA T^GATCA HapII 773 CCGG C^CGG HgaI 1689 GACGC GACGC(5/10) Hin1I 1689 GACGCC G[GA]^CG[CT]C HincII 2010 GTCAAC GT[CT]^[GA]AC HindII 2010 GTCAAC GT[CT]^[GA]AC HpaII 773 CCGG C^CGG MaeII 573 ACGT A^CGT MamI 531 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1525 GGATCC [GA]^GATC[CT] MspA1I 1296 CAGCTG C[AC]G^C[GT]G MspI 773 CCGG C^CGG NaeI 772 GCCGGC GCC^GGC NcoI 842 CCATGG C^CATGG NgoMIV 772 GCCGGC G^CCGGC NheI 95 GCTAGC G^CTAGC NspI 474 ACATGC [GA]CATG^[CT] PshAI 568 GACAAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 1296 CAGCTG CAG^CTG RsaI 552 GTAC GT^AC Tsp45I 86 GTCAC ^GT[GC]AC XhoII 1525 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### Asp700I 1485 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1918 GAAACCCTTC AspHI 1419 GAGCTC G[AT]GC[AT]^C 2039 GAGCTC BanI 669 GGTGCC G^G[CT][GA]CC 2158 GGCACC BglI 947 GCCTGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1752 GCCCCCCAGGC BsiHKAI 1419 GAGCTC G[AT]GC[AT]^C 2039 GAGCTC BsmI 1442 GAATGC GAATGC(1/-1) 522 GCATTC BspMI 2161 ACCTGC ACCTGC(4/8) 2303 ACCTGC CfoI 960 GCGC GCG^C 2050 GCGC EarI 741 CTCTTC CTCTTC(1/4) 828 GAAGAG HaeII 959 GGCGCT [GA]GCGC^[CT] 2049 AGCGCT HhaI 960 GCGC GCG^C 2050 GCGC HinP1I 960 GCGC G^CGC 2050 GCGC Ksp632I 741 CTCTTC CTCTTC(1/4) 828 GAAGAG MaeIII 40 GTAAC ^GT[ACGT]AC 86 GTCAC SacI 1419 GAGCTC GAGCT^C 2039 GAGCTC SbfI 170 CCTGCAGG CCTGCA^GG 948 CCTGCAGG SmlI 605 CTCAAG C^T[CT][GA]AG 1037 CTCAAG Sse8387I 170 CCTGCAGG CCTGCA^GG 948 CCTGCAGG StuI 1182 AGGCCT AGG^CCT 2114 AGGCCT TaqI 590 TCGA T^CGA 1385 TCGA TthHB8I 590 TCGA T^CGA 1385 TCGA XmnI 1485 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1918 GAAACCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AhdI AluI AlwI AlwNI ApaI AspEI AvaII BalI BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstXI Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI Eam1105I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MboI MboII MluNI MnlI MscI MseI MslI MvaI MwoI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp509I TspRI Van91I XcmI