################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## AatII AclI AcsI AcyI AflII AgeI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI AviII AvrII BbeI BbrPI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsiEI BsiWI BsmBI BspLU11I BsrFI BssHII BssSI Bst1107I BstAPI BstZ17I Bsu36I CelII Cfr10I CpoI DraI DraIII EagI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV FbaI FseI FspI HaeII Hin1I KasI KspI MamI MluI NaeI NarI NgoMIV NheI NotI NruI PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI Sse8387I SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### Acc65I 1625 GGTACC G^GTACC AccI 598 GTCTAC GT^[AC][GT]AC AccII 1559 CGCG CG^CG AccIII 545 TCCGGA T^CCGGA AflIII 236 ACACGT A^C[GA][CT]GT AhdI 477 GACTGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp718I 1625 GGTACC G^GTACC AspEI 477 GACTGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 2424 GAGCTC G[AT]GC[AT]^C AvaI 1197 CTCGGG C^[CT]CG[GA]G BbvCI 2330 CCTCAGC CCTCAGC(-5/-2) BcgI 742 CGAACCCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 2460 CCGGG CC^[GC]GG BglI 70 GCCAACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BseAI 545 TCCGGA T^CCGGA BsgI 944 CTGCAC GTGCAG(16/14) BsiHKAI 2424 GAGCTC G[AT]GC[AT]^C BsoBI 1197 CTCGGG C^[CT]CG[GA]G Bsp1407I 1102 TGTACA T^GTACA BspDI 1663 ATCGAT AT^CGAT BspEI 545 TCCGGA T^CCGGA BsrBI 1323 CCGCTC CCGCTC(-3/-3) BsrGI 1102 TGTACA T^GTACA BstBI 740 TTCGAA TT^CGAA BstEII 54 GGTTACC G^GT[ACGT]ACC BstPI 54 GGTTACC G^GT[ACGT]ACC BstUI 1559 CGCG CG^CG BstXI 1816 CCAACTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 1560 GCGC GCG^C ClaI 1663 ATCGAT AT^CGAT DrdI 1900 GACATAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 477 GACTGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 2588 CCTATTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 54 GGTTACC G^GT[ACGT]ACC EcoT22I 1841 ATGCAT ATGCA^T HhaI 1560 GCGC GCG^C HinP1I 1560 GCGC G^CGC HpaI 1192 GTTAAC GTT^AAC KpnI 1625 GGTACC GGTAC^C MfeI 1098 CAATTG C^AATTG MroI 545 TCCGGA T^CCGGA MspA1I 1001 CAGCTG C[AC]G^C[GT]G MunI 1098 CAATTG C^AATTG MvnI 1559 CGCG CG^CG NciI 2460 CCGGG CC^[GC]GG NsiI 1841 ATGCAT ATGCA^T NspV 740 TTCGAA TT^CGAA PshAI 999 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 1001 CAGCTG CAG^CTG SacI 2424 GAGCTC GAGCT^C ScaI 1298 AGTACT AGT^ACT SfuI 740 TTCGAA TT^CGAA SpeI 450 ACTAGT A^CTAGT SspBI 1102 TGTACA T^GTACA SspI 661 AATATT AAT^ATT StuI 2299 AGGCCT AGG^CCT XcmI 1629 CCAGTTCTTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AlwNI 862 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG 2333 CAGCTCCTG AspI 538 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 616 GACTCAGTC BamHI 2472 GGATCC G^GATCC 2559 GGATCC BanII 40 GAGCCC G[GA]GC[CT]^C 2424 GAGCTC BglII 177 AGATCT A^GATCT 2644 AGATCT BspHI 1128 TCATGA T^CATGA 1382 TCATGA DraII 195 GGGACCC [GA]G^G[ACGT]CC[CT] 700 AGGACCC EcoO109I 195 GGGACCC [GA]G^G[ACGT]CC[CT] 700 AGGACCC FauI 1505 CCCGC CCCGC(4/6) 951 GCGGG HgaI 483 GCGTC GACGC(5/10) 1379 GCGTC HindIII 127 AAGCTT A^AGCTT 2019 AAGCTT MseI 1061 TTAA T^TAA 1193 TTAA MslI 237 CACGTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 447 CACACTAGTG NcoI 1832 CCATGG C^CATGG 1998 CCATGG NdeI 2204 CATATG CA^TATG 2664 CATATG PflFI 538 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 616 GACTCAGTC PflMI 861 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1965 CCAGGCAATGG PpuMI 195 GGGACCC [GA]G^G[AT]CC[CT] 700 AGGACCC PstI 1336 CTGCAG CTGCA^G 1748 CTGCAG RcaI 1128 TCATGA T^CATGA 1382 TCATGA SapI 111 GCTCTTC GCTCTTC(1/4) 2164 GCTCTTC SmlI 1519 CTCAAG C^T[CT][GA]AG 2622 CTCAAG SphI 423 GCATGC GCATG^C 1469 GCATGC Tru9I 1061 TTAA T^TAA 1193 TTAA Tth111I 538 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 616 GACTCAGTC Van91I 861 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1965 CCAGGCAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AciI AfaI AluI AlwI Asp700I AvaII BalI BanI BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BsaWI BseRI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI