##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AclI AcsI AcyI AflII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
AviII AvrII BbeI BbrPI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsiEI
BsiWI BsmBI BspLU11I BsrFI BssHII
BssSI Bst1107I BstAPI BstZ17I Bsu36I
CelII Cfr10I CpoI DraI DraIII
EagI EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV FbaI FseI FspI
HaeII Hin1I KasI KspI MamI
MluI NaeI NarI NgoMIV NheI
NotI NruI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfoI SgrAI SmaI
SnaBI Sse8387I SwaI XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 1625 GGTACC G^GTACC
AccI 598 GTCTAC GT^[AC][GT]AC
AccII 1559 CGCG CG^CG
AccIII 545 TCCGGA T^CCGGA
AflIII 236 ACACGT A^C[GA][CT]GT
AhdI 477 GACTGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 1625 GGTACC G^GTACC
AspEI 477 GACTGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2424 GAGCTC G[AT]GC[AT]^C
AvaI 1197 CTCGGG C^[CT]CG[GA]G
BbvCI 2330 CCTCAGC CCTCAGC(-5/-2)
BcgI 742 CGAACCCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 2460 CCGGG CC^[GC]GG
BglI 70 GCCAACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseAI 545 TCCGGA T^CCGGA
BsgI 944 CTGCAC GTGCAG(16/14)
BsiHKAI 2424 GAGCTC G[AT]GC[AT]^C
BsoBI 1197 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1102 TGTACA T^GTACA
BspDI 1663 ATCGAT AT^CGAT
BspEI 545 TCCGGA T^CCGGA
BsrBI 1323 CCGCTC CCGCTC(-3/-3)
BsrGI 1102 TGTACA T^GTACA
BstBI 740 TTCGAA TT^CGAA
BstEII 54 GGTTACC G^GT[ACGT]ACC
BstPI 54 GGTTACC G^GT[ACGT]ACC
BstUI 1559 CGCG CG^CG
BstXI 1816 CCAACTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1560 GCGC GCG^C
ClaI 1663 ATCGAT AT^CGAT
DrdI 1900 GACATAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 477 GACTGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2588 CCTATTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 54 GGTTACC G^GT[ACGT]ACC
EcoT22I 1841 ATGCAT ATGCA^T
HhaI 1560 GCGC GCG^C
HinP1I 1560 GCGC G^CGC
HpaI 1192 GTTAAC GTT^AAC
KpnI 1625 GGTACC GGTAC^C
MfeI 1098 CAATTG C^AATTG
MroI 545 TCCGGA T^CCGGA
MspA1I 1001 CAGCTG C[AC]G^C[GT]G
MunI 1098 CAATTG C^AATTG
MvnI 1559 CGCG CG^CG
NciI 2460 CCGGG CC^[GC]GG
NsiI 1841 ATGCAT ATGCA^T
NspV 740 TTCGAA TT^CGAA
PshAI 999 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1001 CAGCTG CAG^CTG
SacI 2424 GAGCTC GAGCT^C
ScaI 1298 AGTACT AGT^ACT
SfuI 740 TTCGAA TT^CGAA
SpeI 450 ACTAGT A^CTAGT
SspBI 1102 TGTACA T^GTACA
SspI 661 AATATT AAT^ATT
StuI 2299 AGGCCT AGG^CCT
XcmI 1629 CCAGTTCTTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwNI 862 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
2333 CAGCTCCTG
AspI 538 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
616 GACTCAGTC
BamHI 2472 GGATCC G^GATCC
2559 GGATCC
BanII 40 GAGCCC G[GA]GC[CT]^C
2424 GAGCTC
BglII 177 AGATCT A^GATCT
2644 AGATCT
BspHI 1128 TCATGA T^CATGA
1382 TCATGA
DraII 195 GGGACCC [GA]G^G[ACGT]CC[CT]
700 AGGACCC
EcoO109I 195 GGGACCC [GA]G^G[ACGT]CC[CT]
700 AGGACCC
FauI 1505 CCCGC CCCGC(4/6)
951 GCGGG
HgaI 483 GCGTC GACGC(5/10)
1379 GCGTC
HindIII 127 AAGCTT A^AGCTT
2019 AAGCTT
MseI 1061 TTAA T^TAA
1193 TTAA
MslI 237 CACGTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
447 CACACTAGTG
NcoI 1832 CCATGG C^CATGG
1998 CCATGG
NdeI 2204 CATATG CA^TATG
2664 CATATG
PflFI 538 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
616 GACTCAGTC
PflMI 861 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1965 CCAGGCAATGG
PpuMI 195 GGGACCC [GA]G^G[AT]CC[CT]
700 AGGACCC
PstI 1336 CTGCAG CTGCA^G
1748 CTGCAG
RcaI 1128 TCATGA T^CATGA
1382 TCATGA
SapI 111 GCTCTTC GCTCTTC(1/4)
2164 GCTCTTC
SmlI 1519 CTCAAG C^T[CT][GA]AG
2622 CTCAAG
SphI 423 GCATGC GCATG^C
1469 GCATGC
Tru9I 1061 TTAA T^TAA
1193 TTAA
Tth111I 538 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
616 GACTCAGTC
Van91I 861 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1965 CCAGGCAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AfaI AluI AlwI Asp700I
AvaII BalI BanI BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BsaWI BseRI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DsaI
EaeI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI