##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII AccI AccII AclI AflII
AgeI Aor51HI ApaI AscI AseI
AsnI Asp700I AspI AviII AvrII
BbeI BbrPI BbvCI BclI BfrI
BglI BlnI BsaBI BsiWI BsmI
Bsp1407I BspDI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstUI BstXI BstZ17I Cfr10I
ClaI CpoI DraI DrdI Eco47III
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HindIII HpaI KasI
KspI MamI MluI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 230 GGTACC G^GTACC
AccIII 2344 TCCGGA T^CCGGA
AcsI 1180 AAATTC [GA]^AATT[CT]
AflIII 1021 ACGTGT A^C[GA][CT]GT
Alw44I 1639 GTGCAC G^TGCAC
AlwNI 2022 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2153 CCTGAGG CC^T[ACGT]AGG
ApaLI 1639 GTGCAC G^TGCAC
ApoI 1180 AAATTC [GA]^AATT[CT]
Asp718I 230 GGTACC G^GTACC
AvaI 1651 CCCGAG C^[CT]CG[GA]G
BamHI 331 GGATCC G^GATCC
BcgI 1948 GCACGAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 957 GGATAC GTATCC(6/5)
BfaI 1644 CTAG C^TAG
BglII 365 AGATCT A^GATCT
BsaAI 2242 CACGTA [CT]AC^GT[GA]
BsaWI 2344 TCCGGA [AT]^CCGG[AT]
BseAI 2344 TCCGGA T^CCGGA
BseRI 1614 GAGGAG GAGGAG(10/8)
BsgI 489 GTGCAG GTGCAG(16/14)
BsiEI 2170 CGGCCG CG[GA][CT]^CG
BsoBI 1651 CCCGAG C^[CT]CG[GA]G
BspEI 2344 TCCGGA T^CCGGA
BstEII 167 GGTGACC G^GT[ACGT]ACC
BstPI 167 GGTGACC G^GT[ACGT]ACC
Bsu36I 2153 CCTGAGG CC^T[ACGT]AGG
EagI 2170 CGGCCG C^GGCCG
EclXI 2170 CGGCCG C^GGCCG
Eco52I 2170 CGGCCG C^GGCCG
Eco81I 2153 CCTGAGG CC^T[ACGT]AGG
EcoNI 2417 CCTACAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 167 GGTGACC G^GT[ACGT]ACC
HincII 2034 GTCAAC GT[CT]^[GA]AC
HindII 2034 GTCAAC GT[CT]^[GA]AC
KpnI 230 GGTACC GGTAC^C
MaeI 1644 CTAG C^TAG
MfeI 1735 CAATTG C^AATTG
MroI 2344 TCCGGA T^CCGGA
MunI 1735 CAATTG C^AATTG
PflMI 1467 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 216 GAGCTC GAGCT^C
SexAI 289 ACCTGGT A^CC[AT]GGT
StuI 2106 AGGCCT AGG^CCT
Van91I 1467 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 1406 GGCGTC G[GA]^CG[CT]C
2094 GACGCC
AhdI 303 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1017 GACAACGTGTC
AspEI 303 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1017 GACAACGTGTC
BanI 230 GGTACC G^G[CT][GA]CC
2229 GGCACC
BcnI 526 CCGGG CC^[GC]GG
1609 CCGGG
BlpI 30 GCTGAGC GC^T[ACGT]AGC
1943 GCTGAGC
BpmI 1545 CTGGAG CTGGAG(16/14)
2207 CTGGAG
Bpu1102I 30 GCTGAGC GC^T[ACGT]AGC
1943 GCTGAGC
BsaHI 1406 GGCGTC G[GA]^CG[CT]C
2094 GACGCC
BsmBI 256 GAGACG CGTCTC(1/5)
2092 GAGACG
BspHI 1258 TCATGA T^CATGA
1879 TCATGA
BsrI 431 ACTGG ACTGG(1/-1)
773 ACTGG
CelII 30 GCTGAGC GC^T[ACGT]AGC
1943 GCTGAGC
CfoI 1885 GCGC GCG^C
2061 GCGC
DraIII 1985 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
2113 CACGGAGTG
Eam1105I 303 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1017 GACAACGTGTC
HhaI 1885 GCGC GCG^C
2061 GCGC
Hin1I 1406 GGCGTC G[GA]^CG[CT]C
2094 GACGCC
HinP1I 1885 GCGC G^CGC
2061 GCGC
MseI 1522 TTAA T^TAA
1702 TTAA
NciI 526 CCGGG CC^[GC]GG
1609 CCGGG
PshAI 770 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1820 GACAGATGTC
PstI 594 CTGCAG CTGCA^G
1292 CTGCAG
PvuII 1941 CAGCTG CAG^CTG
2391 CAGCTG
RcaI 1258 TCATGA T^CATGA
1879 TCATGA
SfcI 594 CTGCAG C^T[GA][CT]AG
1292 CTGCAG
TaqI 1459 TCGA T^CGA
2281 TCGA
TfiI 381 GAATC G^A[AT]TC
1158 GATTC
Tru9I 1522 TTAA T^TAA
1702 TTAA
TthHB8I 1459 TCGA T^CGA
2281 TCGA
XcmI 754 CCATCGTTGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1843 CCAACATGATCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BalI BanII BbsI BbvI
BmyI BpuAI BsaI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BssKI BssSI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI StyI
TseI Tsp45I Tsp509I TspRI XhoII