################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII AccI AccII AclI AflII AgeI Aor51HI ApaI AscI AseI AsnI Asp700I AspI AviII AvrII BbeI BbrPI BbvCI BclI BfrI BglI BlnI BsaBI BsiWI BsmI Bsp1407I BspDI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Cfr10I ClaI CpoI DraI DrdI Eco47III EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HindIII HpaI KasI KspI MamI MluI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 230 GGTACC G^GTACC AccIII 2344 TCCGGA T^CCGGA AcsI 1180 AAATTC [GA]^AATT[CT] AflIII 1021 ACGTGT A^C[GA][CT]GT Alw44I 1639 GTGCAC G^TGCAC AlwNI 2022 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 2153 CCTGAGG CC^T[ACGT]AGG ApaLI 1639 GTGCAC G^TGCAC ApoI 1180 AAATTC [GA]^AATT[CT] Asp718I 230 GGTACC G^GTACC AvaI 1651 CCCGAG C^[CT]CG[GA]G BamHI 331 GGATCC G^GATCC BcgI 1948 GCACGAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 957 GGATAC GTATCC(6/5) BfaI 1644 CTAG C^TAG BglII 365 AGATCT A^GATCT BsaAI 2242 CACGTA [CT]AC^GT[GA] BsaWI 2344 TCCGGA [AT]^CCGG[AT] BseAI 2344 TCCGGA T^CCGGA BseRI 1614 GAGGAG GAGGAG(10/8) BsgI 489 GTGCAG GTGCAG(16/14) BsiEI 2170 CGGCCG CG[GA][CT]^CG BsoBI 1651 CCCGAG C^[CT]CG[GA]G BspEI 2344 TCCGGA T^CCGGA BstEII 167 GGTGACC G^GT[ACGT]ACC BstPI 167 GGTGACC G^GT[ACGT]ACC Bsu36I 2153 CCTGAGG CC^T[ACGT]AGG EagI 2170 CGGCCG C^GGCCG EclXI 2170 CGGCCG C^GGCCG Eco52I 2170 CGGCCG C^GGCCG Eco81I 2153 CCTGAGG CC^T[ACGT]AGG EcoNI 2417 CCTACAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 167 GGTGACC G^GT[ACGT]ACC HincII 2034 GTCAAC GT[CT]^[GA]AC HindII 2034 GTCAAC GT[CT]^[GA]AC KpnI 230 GGTACC GGTAC^C MaeI 1644 CTAG C^TAG MfeI 1735 CAATTG C^AATTG MroI 2344 TCCGGA T^CCGGA MunI 1735 CAATTG C^AATTG PflMI 1467 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 216 GAGCTC GAGCT^C SexAI 289 ACCTGGT A^CC[AT]GGT StuI 2106 AGGCCT AGG^CCT Van91I 1467 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AcyI 1406 GGCGTC G[GA]^CG[CT]C 2094 GACGCC AhdI 303 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1017 GACAACGTGTC AspEI 303 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1017 GACAACGTGTC BanI 230 GGTACC G^G[CT][GA]CC 2229 GGCACC BcnI 526 CCGGG CC^[GC]GG 1609 CCGGG BlpI 30 GCTGAGC GC^T[ACGT]AGC 1943 GCTGAGC BpmI 1545 CTGGAG CTGGAG(16/14) 2207 CTGGAG Bpu1102I 30 GCTGAGC GC^T[ACGT]AGC 1943 GCTGAGC BsaHI 1406 GGCGTC G[GA]^CG[CT]C 2094 GACGCC BsmBI 256 GAGACG CGTCTC(1/5) 2092 GAGACG BspHI 1258 TCATGA T^CATGA 1879 TCATGA BsrI 431 ACTGG ACTGG(1/-1) 773 ACTGG CelII 30 GCTGAGC GC^T[ACGT]AGC 1943 GCTGAGC CfoI 1885 GCGC GCG^C 2061 GCGC DraIII 1985 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG 2113 CACGGAGTG Eam1105I 303 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1017 GACAACGTGTC HhaI 1885 GCGC GCG^C 2061 GCGC Hin1I 1406 GGCGTC G[GA]^CG[CT]C 2094 GACGCC HinP1I 1885 GCGC G^CGC 2061 GCGC MseI 1522 TTAA T^TAA 1702 TTAA NciI 526 CCGGG CC^[GC]GG 1609 CCGGG PshAI 770 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1820 GACAGATGTC PstI 594 CTGCAG CTGCA^G 1292 CTGCAG PvuII 1941 CAGCTG CAG^CTG 2391 CAGCTG RcaI 1258 TCATGA T^CATGA 1879 TCATGA SfcI 594 CTGCAG C^T[GA][CT]AG 1292 CTGCAG TaqI 1459 TCGA T^CGA 2281 TCGA TfiI 381 GAATC G^A[AT]TC 1158 GATTC Tru9I 1522 TTAA T^TAA 1702 TTAA TthHB8I 1459 TCGA T^CGA 2281 TCGA XcmI 754 CCATCGTTGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1843 CCAACATGATCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaII BalI BanII BbsI BbvI BmyI BpuAI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BssKI BssSI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI RsaI SapI Sau3AI Sau96I ScrFI SfaNI StyI TseI Tsp45I Tsp509I TspRI XhoII