################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## Acc65I AccI AccIII AclI AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspI BamHI BbrPI BbsI BbvCI BciVI BglII BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DraI DrdI DsaI EagI EclXI Eco47III Eco52I EcoRI EcoT22I FseI HincII HindII HindIII HpaI KpnI KspI MamI MroI NaeI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 71 enzymes) # ################################### AatII 629 GACGTC GACGT^C AccII 469 CGCG CG^CG AcsI 340 AAATTC [GA]^AATT[CT] AflII 989 CTTAAG C^TTAAG AflIII 468 ACGCGT A^C[GA][CT]GT AhdI 629 GACGTCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 45 GGATC GGATC(4/5) AocI 1208 CCTGAGG CC^T[ACGT]AGG ApoI 340 AAATTC [GA]^AATT[CT] AspEI 629 GACGTCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 674 GAGCTC G[AT]GC[AT]^C AvaI 1004 CCCGAG C^[CT]CG[GA]G AviII 475 TGCGCA TGC^GCA AvrII 1372 CCTAGG C^CTAGG BalI 1536 TGGCCA TGG^CCA BanII 674 GAGCTC G[GA]GC[CT]^C BbeI 1233 GGCGCC GGCGC^C BcgI 1065 GCAGGGCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1452 TGATCA T^GATCA BfrI 989 CTTAAG C^TTAAG BlnI 1372 CCTAGG C^CTAGG BpmI 809 CTCCAG CTGGAG(16/14) BseRI 774 CTCCTC GAGGAG(10/8) BsiHKAI 674 GAGCTC G[AT]GC[AT]^C BsmBI 91 GAGACG CGTCTC(1/5) BsoBI 1004 CCCGAG C^[CT]CG[GA]G BsrBI 1499 GAGCGG CCGCTC(-3/-3) BsrFI 396 ACCGGC [GA]^CCGG[CT] BssSI 115 CACGAG CACGAG(-5/-1) BstAPI 1384 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1213 GGTTACC G^GT[ACGT]ACC BstPI 1213 GGTTACC G^GT[ACGT]ACC BstUI 469 CGCG CG^CG BstYI 45 GGATCT [GA]^GATC[CT] Bsu36I 1208 CCTGAGG CC^T[ACGT]AGG Cfr10I 396 ACCGGC [GA]^CCGG[CT] DraIII 189 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 1536 TGGCCA [CT]^GGCC[GA] Eam1105I 629 GACGTCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 239 GAAGAG CTCTTC(1/4) Eco81I 1208 CCTGAGG CC^T[ACGT]AGG EcoO65I 1213 GGTTACC G^GT[ACGT]ACC EcoRV 1556 GATATC GAT^ATC FauI 1168 CCCGC CCCGC(4/6) FbaI 1452 TGATCA T^GATCA FspI 475 TGCGCA TGC^GCA HaeII 1233 GGCGCC [GA]GCGC^[CT] KasI 1233 GGCGCC G^GCGCC Ksp632I 239 GAAGAG CTCTTC(1/4) MfeI 1593 CAATTG C^AATTG MflI 45 GGATCT [GA]^GATC[CT] MluI 468 ACGCGT A^CGCGT MluNI 1536 TGGCCA TGG^CCA MscI 1536 TGGCCA TGG^CCA MunI 1593 CAATTG C^AATTG MvnI 469 CGCG CG^CG NarI 1233 GGCGCC GG^CGCC NdeI 689 CATATG CA^TATG PpuMI 312 AGGTCCT [GA]G^G[AT]CC[CT] PvuII 368 CAGCTG CAG^CTG SacI 674 GAGCTC GAGCT^C SbfI 1062 CCTGCAGG CCTGCA^GG ScaI 29 AGTACT AGT^ACT SexAI 190 ACCTGGT A^CC[AT]GGT SfoI 1233 GGCGCC GGC^GCC Sse8387I 1062 CCTGCAGG CCTGCA^GG StuI 1375 AGGCCT AGG^CCT TaqI 357 TCGA T^CGA TthHB8I 357 TCGA T^CGA XcmI 378 CCAATTCCCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 45 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AlwNI 737 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG 1385 CAGAGCCTG BfaI 761 CTAG C^TAG 1373 CTAG BglI 836 GCCTGCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1495 GCCGGAGCGGC BmyI 674 GAGCTC G[AGT]GC[ACT]^C 1316 GGGCAC Bsp1286I 674 GAGCTC G[AGT]GC[ACT]^C 1316 GGGCAC BstXI 421 CCAAACGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 847 CCACGTCAATGG CfoI 476 GCGC GCG^C 1234 GCGC DpnII 46 GATC ^GATC 1453 GATC EcoNI 1218 CCTACCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1321 CCTGTCCAAGG HgaI 93 GACGC GACGC(5/10) 417 GCGTC HhaI 476 GCGC GCG^C 1234 GCGC HinP1I 476 GCGC G^CGC 1234 GCGC MaeI 761 CTAG C^TAG 1373 CTAG MboI 46 GATC ^GATC 1453 GATC MspA1I 368 CAGCTG C[AC]G^C[GT]G 1109 CAGCGG NdeII 46 GATC ^GATC 1453 GATC NspI 868 GCATGT [GA]CATG^[CT] 1278 ACATGC PflMI 188 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 736 CCAGAGACTGG Sau3AI 46 GATC ^GATC 1453 GATC Van91I 188 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 736 CCAGAGACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AciI AcyI AfaI AluI AvaII BanI BbvI BcnI BlpI Bpu1102I BsaHI BsaJI BsiYI BslI BsmAI BsmFI BspMI BsrI BssKI BstF5I BstNI Cac8I CelII Cfr13I DdeI DraII EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII Hin1I HinfI HpaII HphI ItaI MaeII MaeIII MboII MnlI MseI MslI MspI MvaI MwoI NciI NlaIII NlaIV PleI PstI RsaI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI