##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
Acc65I AccI AccIII AclI AgeI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
BamHI BbrPI BbsI BbvCI BciVI
BglII BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DrdI DsaI EagI EclXI Eco47III
Eco52I EcoRI EcoT22I FseI HincII
HindII HindIII HpaI KpnI KspI
MamI MroI NaeI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 71 enzymes) #
###################################
AatII 629 GACGTC GACGT^C
AccII 469 CGCG CG^CG
AcsI 340 AAATTC [GA]^AATT[CT]
AflII 989 CTTAAG C^TTAAG
AflIII 468 ACGCGT A^C[GA][CT]GT
AhdI 629 GACGTCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 45 GGATC GGATC(4/5)
AocI 1208 CCTGAGG CC^T[ACGT]AGG
ApoI 340 AAATTC [GA]^AATT[CT]
AspEI 629 GACGTCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 674 GAGCTC G[AT]GC[AT]^C
AvaI 1004 CCCGAG C^[CT]CG[GA]G
AviII 475 TGCGCA TGC^GCA
AvrII 1372 CCTAGG C^CTAGG
BalI 1536 TGGCCA TGG^CCA
BanII 674 GAGCTC G[GA]GC[CT]^C
BbeI 1233 GGCGCC GGCGC^C
BcgI 1065 GCAGGGCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1452 TGATCA T^GATCA
BfrI 989 CTTAAG C^TTAAG
BlnI 1372 CCTAGG C^CTAGG
BpmI 809 CTCCAG CTGGAG(16/14)
BseRI 774 CTCCTC GAGGAG(10/8)
BsiHKAI 674 GAGCTC G[AT]GC[AT]^C
BsmBI 91 GAGACG CGTCTC(1/5)
BsoBI 1004 CCCGAG C^[CT]CG[GA]G
BsrBI 1499 GAGCGG CCGCTC(-3/-3)
BsrFI 396 ACCGGC [GA]^CCGG[CT]
BssSI 115 CACGAG CACGAG(-5/-1)
BstAPI 1384 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1213 GGTTACC G^GT[ACGT]ACC
BstPI 1213 GGTTACC G^GT[ACGT]ACC
BstUI 469 CGCG CG^CG
BstYI 45 GGATCT [GA]^GATC[CT]
Bsu36I 1208 CCTGAGG CC^T[ACGT]AGG
Cfr10I 396 ACCGGC [GA]^CCGG[CT]
DraIII 189 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1536 TGGCCA [CT]^GGCC[GA]
Eam1105I 629 GACGTCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 239 GAAGAG CTCTTC(1/4)
Eco81I 1208 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1213 GGTTACC G^GT[ACGT]ACC
EcoRV 1556 GATATC GAT^ATC
FauI 1168 CCCGC CCCGC(4/6)
FbaI 1452 TGATCA T^GATCA
FspI 475 TGCGCA TGC^GCA
HaeII 1233 GGCGCC [GA]GCGC^[CT]
KasI 1233 GGCGCC G^GCGCC
Ksp632I 239 GAAGAG CTCTTC(1/4)
MfeI 1593 CAATTG C^AATTG
MflI 45 GGATCT [GA]^GATC[CT]
MluI 468 ACGCGT A^CGCGT
MluNI 1536 TGGCCA TGG^CCA
MscI 1536 TGGCCA TGG^CCA
MunI 1593 CAATTG C^AATTG
MvnI 469 CGCG CG^CG
NarI 1233 GGCGCC GG^CGCC
NdeI 689 CATATG CA^TATG
PpuMI 312 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 368 CAGCTG CAG^CTG
SacI 674 GAGCTC GAGCT^C
SbfI 1062 CCTGCAGG CCTGCA^GG
ScaI 29 AGTACT AGT^ACT
SexAI 190 ACCTGGT A^CC[AT]GGT
SfoI 1233 GGCGCC GGC^GCC
Sse8387I 1062 CCTGCAGG CCTGCA^GG
StuI 1375 AGGCCT AGG^CCT
TaqI 357 TCGA T^CGA
TthHB8I 357 TCGA T^CGA
XcmI 378 CCAATTCCCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 45 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwNI 737 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
1385 CAGAGCCTG
BfaI 761 CTAG C^TAG
1373 CTAG
BglI 836 GCCTGCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1495 GCCGGAGCGGC
BmyI 674 GAGCTC G[AGT]GC[ACT]^C
1316 GGGCAC
Bsp1286I 674 GAGCTC G[AGT]GC[ACT]^C
1316 GGGCAC
BstXI 421 CCAAACGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
847 CCACGTCAATGG
CfoI 476 GCGC GCG^C
1234 GCGC
DpnII 46 GATC ^GATC
1453 GATC
EcoNI 1218 CCTACCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1321 CCTGTCCAAGG
HgaI 93 GACGC GACGC(5/10)
417 GCGTC
HhaI 476 GCGC GCG^C
1234 GCGC
HinP1I 476 GCGC G^CGC
1234 GCGC
MaeI 761 CTAG C^TAG
1373 CTAG
MboI 46 GATC ^GATC
1453 GATC
MspA1I 368 CAGCTG C[AC]G^C[GT]G
1109 CAGCGG
NdeII 46 GATC ^GATC
1453 GATC
NspI 868 GCATGT [GA]CATG^[CT]
1278 ACATGC
PflMI 188 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
736 CCAGAGACTGG
Sau3AI 46 GATC ^GATC
1453 GATC
Van91I 188 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
736 CCAGAGACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcyI AfaI AluI AvaII
BanI BbvI BcnI BlpI Bpu1102I
BsaHI BsaJI BsiYI BslI BsmAI
BsmFI BspMI BsrI BssKI BstF5I
BstNI Cac8I CelII Cfr13I DdeI
DraII EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII Hin1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboII MnlI MseI MslI MspI
MvaI MwoI NciI NlaIII NlaIV
PleI PstI RsaI Sau96I ScrFI
SfaNI SfcI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI