##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI AocI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BamHI BcgI BciVI BclI BfaI
BfrI BlnI BlpI Bpu1102I BsaBI
BsiEI BsiWI BsmBI BspDI BspHI
BspLU11I BsrDI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI EagI
Eam1105I EclXI Eco52I Eco81I EcoO65I
EcoT22I FbaI FseI HincII HindII
HpaI KpnI KspI MaeI MamI
MfeI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tth111I Van91I XbaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccII 700 CGCG CG^CG
AccIII 935 TCCGGA T^CCGGA
AcsI 1161 GAATTC [GA]^AATT[CT]
AcyI 229 GGCGCC G[GA]^CG[CT]C
ApaI 891 GGGCCC GGGCC^C
ApoI 1161 GAATTC [GA]^AATT[CT]
Asp700I 1499 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1324 TGCGCA TGC^GCA
BalI 1436 TGGCCA TGG^CCA
BbeI 229 GGCGCC GGCGC^C
BbrPI 2040 CACGTG CAC^GTG
BbvCI 873 CCTCAGC CCTCAGC(-5/-2)
BglII 424 AGATCT A^GATCT
BsaAI 2040 CACGTG [CT]AC^GT[GA]
BsaHI 229 GGCGCC G[GA]^CG[CT]C
BseAI 935 TCCGGA T^CCGGA
BsmI 292 GCATTC GAATGC(1/-1)
Bsp1407I 1648 TGTACA T^GTACA
BspEI 935 TCCGGA T^CCGGA
BsrGI 1648 TGTACA T^GTACA
BstUI 700 CGCG CG^CG
EarI 1538 GAAGAG CTCTTC(1/4)
EcoRI 1161 GAATTC G^AATTC
FspI 1324 TGCGCA TGC^GCA
HgaI 928 GACGC GACGC(5/10)
Hin1I 229 GGCGCC G[GA]^CG[CT]C
KasI 229 GGCGCC G^GCGCC
Ksp632I 1538 GAAGAG CTCTTC(1/4)
MaeII 2041 ACGT A^CGT
MluI 699 ACGCGT A^CGCGT
MluNI 1436 TGGCCA TGG^CCA
MroI 935 TCCGGA T^CCGGA
MscI 1436 TGGCCA TGG^CCA
MvnI 700 CGCG CG^CG
NarI 229 GGCGCC GG^CGCC
NspI 1387 ACATGC [GA]CATG^[CT]
PaeR7I 607 CTCGAG C^TCGAG
PmaCI 2040 CACGTG CAC^GTG
PmlI 2040 CACGTG CAC^GTG
SfoI 229 GGCGCC GGC^GCC
SmaI 736 CCCGGG CCC^GGG
SspBI 1648 TGTACA T^GTACA
TaqI 608 TCGA T^CGA
Tsp509I 1162 AATT ^AATT
TthHB8I 608 TCGA T^CGA
XhoI 607 CTCGAG C^TCGAG
XmaCI 736 CCCGGG C^CCGGG
XmaI 736 CCCGGG C^CCGGG
XmnI 1499 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflIII 699 ACGCGT A^C[GA][CT]GT
2041 ACGTGT
Alw44I 1202 GTGCAC G^TGCAC
1695 GTGCAC
ApaLI 1202 GTGCAC G^TGCAC
1695 GTGCAC
BglI 632 GCCCCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
708 GCCTCTGAGGC
BsgI 365 CTGCAC GTGCAG(16/14)
882 GTGCAG
BsrBI 1521 GAGCGG CCGCTC(-3/-3)
1599 GAGCGG
BsrFI 551 ACCGGC [GA]^CCGG[CT]
878 GCCGGT
BstXI 758 CCATGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1243 CCAATGGCTTGG
Cfr10I 551 ACCGGC [GA]^CCGG[CT]
878 GCCGGT
DraIII 368 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
1147 CACTGGGTG
DrdI 1550 GACCACCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1731 GACCCCTTTGTC
EcoNI 672 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
918 CCTGGGCCAGG
EcoRV 1842 GATATC GAT^ATC
1878 GATATC
HindIII 1269 AAGCTT A^AGCTT
1305 AAGCTT
MseI 1795 TTAA T^TAA
1822 TTAA
SexAI 1461 ACCAGGT A^CC[AT]GGT
2059 ACCTGGT
Tru9I 1795 TTAA T^TAA
1822 TTAA
XcmI 533 CCAGCAGCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
758 CCATGCCCATGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
Aor51HI AspHI AvaI AvaII BanI
BanII BbsI BbvI BcnI BmyI
BpmI BpuAI BsaI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI Eco47III EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI TseI Tsp45I TspRI
XhoII