##################################
# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI AhdI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BanI BbeI BbvCI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspEI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV FbaI FseI
FspI HaeII HgaI Hin1I HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacI SacII
SapI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI StuI SwaI Tsp509I Tth111I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 81 GTCGAC GT^[AC][GT]AC
AccII 1398 CGCG CG^CG
Alw44I 430 GTGCAC G^TGCAC
ApaI 248 GGGCCC GGGCC^C
ApaLI 430 GTGCAC G^TGCAC
AvaI 1893 CCCGGG C^[CT]CG[GA]G
BcgI 835 CGATGTGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BseRI 1935 GAGGAG GAGGAG(10/8)
BsgI 1377 CTGCAC GTGCAG(16/14)
BsoBI 1893 CCCGGG C^[CT]CG[GA]G
BspDI 833 ATCGAT AT^CGAT
BspHI 792 TCATGA T^CATGA
BspLU11I 396 ACATGT A^CATGT
BsrBI 1322 GAGCGG CCGCTC(-3/-3)
BssSI 1750 CACGAG CACGAG(-5/-1)
BstUI 1398 CGCG CG^CG
BstXI 596 CCACAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1399 GCGC GCG^C
ClaI 833 ATCGAT AT^CGAT
EaeI 822 TGGCCG [CT]^GGCC[GA]
EcoT22I 804 ATGCAT ATGCA^T
HhaI 1399 GCGC GCG^C
HinP1I 1399 GCGC G^CGC
MvnI 1398 CGCG CG^CG
NsiI 804 ATGCAT ATGCA^T
PflMI 1290 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1270 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 66 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 792 TCATGA T^CATGA
SalI 81 GTCGAC G^TCGAC
SbfI 1265 CCTGCAGG CCTGCA^GG
ScaI 385 AGTACT AGT^ACT
SmaI 1893 CCCGGG CCC^GGG
SmlI 1073 CTCAAG C^T[CT][GA]AG
Sse8387I 1265 CCTGCAGG CCTGCA^GG
TfiI 1707 GAATC G^A[AT]TC
Van91I 1290 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1893 CCCGGG C^CCGGG
XmaI 1893 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwNI 416 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
900 CAGCCCCTG
AspHI 430 GTGCAC G[AT]GC[AT]^C
720 GAGCAC
BamHI 537 GGATCC G^GATCC
1244 GGATCC
BanII 248 GGGCCC G[GA]GC[CT]^C
1165 GAGCCC
BbrPI 59 CACGTG CAC^GTG
921 CACGTG
BbsI 1032 GAAGAC GAAGAC(2/6)
788 GTCTTC
BglI 849 GCCTGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1505 GCCCATTTGGC
BpuAI 1032 GAAGAC GAAGAC(2/6)
788 GTCTTC
BsaAI 59 CACGTG [CT]AC^GT[GA]
921 CACGTG
BsaI 1366 GAGACC GGTCTC(1/5)
1301 GGTCTC
BsiHKAI 430 GTGCAC G[AT]GC[AT]^C
720 GAGCAC
BspMI 949 ACCTGC ACCTGC(4/8)
1268 GCAGGT
BsrDI 99 CATTGC GCAATG(2/0)
1124 CATTGC
BstYI 537 GGATCC [GA]^GATC[CT]
1244 GGATCC
DsaI 281 CCACGG C^C[GA][CT]GG
825 CCGTGG
EarI 616 GAAGAG CTCTTC(1/4)
1344 GAAGAG
EcoT14I 49 CCAAGG C^C[AT][AT]GG
253 CCTTGG
HindIII 189 AAGCTT A^AGCTT
404 AAGCTT
Ksp632I 616 GAAGAG CTCTTC(1/4)
1344 GAAGAG
MflI 537 GGATCC [GA]^GATC[CT]
1244 GGATCC
MspA1I 677 CAGCTG C[AC]G^C[GT]G
768 CAGCTG
NspI 396 ACATGT [GA]CATG^[CT]
1459 GCATGT
PmaCI 59 CACGTG CAC^GTG
921 CACGTG
PmlI 59 CACGTG CAC^GTG
921 CACGTG
PvuII 677 CAGCTG CAG^CTG
768 CAGCTG
SfaNI 956 GATGC GCATC(5/9)
1886 GCATC
StyI 49 CCAAGG C^C[AT][AT]GG
253 CCTTGG
XcmI 244 CCAGGGGCCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1196 CCACCTTTGCCCTGG
XhoII 537 GGATCC [GA]^GATC[CT]
1244 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AvaII BbvI BcnI BfaI BmyI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfcI TaqI Tru9I TseI Tsp45I
TspRI TthHB8I