################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AflII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BanI BbeI BbvCI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspEI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FbaI FseI FspI HaeII HgaI Hin1I HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RsrII SacI SacII SapI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspBI SspI StuI SwaI Tsp509I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccI 81 GTCGAC GT^[AC][GT]AC AccII 1398 CGCG CG^CG Alw44I 430 GTGCAC G^TGCAC ApaI 248 GGGCCC GGGCC^C ApaLI 430 GTGCAC G^TGCAC AvaI 1893 CCCGGG C^[CT]CG[GA]G BcgI 835 CGATGTGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BseRI 1935 GAGGAG GAGGAG(10/8) BsgI 1377 CTGCAC GTGCAG(16/14) BsoBI 1893 CCCGGG C^[CT]CG[GA]G BspDI 833 ATCGAT AT^CGAT BspHI 792 TCATGA T^CATGA BspLU11I 396 ACATGT A^CATGT BsrBI 1322 GAGCGG CCGCTC(-3/-3) BssSI 1750 CACGAG CACGAG(-5/-1) BstUI 1398 CGCG CG^CG BstXI 596 CCACAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 1399 GCGC GCG^C ClaI 833 ATCGAT AT^CGAT EaeI 822 TGGCCG [CT]^GGCC[GA] EcoT22I 804 ATGCAT ATGCA^T HhaI 1399 GCGC GCG^C HinP1I 1399 GCGC G^CGC MvnI 1398 CGCG CG^CG NsiI 804 ATGCAT ATGCA^T PflMI 1290 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1270 AGGTCCT [GA]G^G[AT]CC[CT] PshAI 66 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 792 TCATGA T^CATGA SalI 81 GTCGAC G^TCGAC SbfI 1265 CCTGCAGG CCTGCA^GG ScaI 385 AGTACT AGT^ACT SmaI 1893 CCCGGG CCC^GGG SmlI 1073 CTCAAG C^T[CT][GA]AG Sse8387I 1265 CCTGCAGG CCTGCA^GG TfiI 1707 GAATC G^A[AT]TC Van91I 1290 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 1893 CCCGGG C^CCGGG XmaI 1893 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AlwNI 416 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG 900 CAGCCCCTG AspHI 430 GTGCAC G[AT]GC[AT]^C 720 GAGCAC BamHI 537 GGATCC G^GATCC 1244 GGATCC BanII 248 GGGCCC G[GA]GC[CT]^C 1165 GAGCCC BbrPI 59 CACGTG CAC^GTG 921 CACGTG BbsI 1032 GAAGAC GAAGAC(2/6) 788 GTCTTC BglI 849 GCCTGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1505 GCCCATTTGGC BpuAI 1032 GAAGAC GAAGAC(2/6) 788 GTCTTC BsaAI 59 CACGTG [CT]AC^GT[GA] 921 CACGTG BsaI 1366 GAGACC GGTCTC(1/5) 1301 GGTCTC BsiHKAI 430 GTGCAC G[AT]GC[AT]^C 720 GAGCAC BspMI 949 ACCTGC ACCTGC(4/8) 1268 GCAGGT BsrDI 99 CATTGC GCAATG(2/0) 1124 CATTGC BstYI 537 GGATCC [GA]^GATC[CT] 1244 GGATCC DsaI 281 CCACGG C^C[GA][CT]GG 825 CCGTGG EarI 616 GAAGAG CTCTTC(1/4) 1344 GAAGAG EcoT14I 49 CCAAGG C^C[AT][AT]GG 253 CCTTGG HindIII 189 AAGCTT A^AGCTT 404 AAGCTT Ksp632I 616 GAAGAG CTCTTC(1/4) 1344 GAAGAG MflI 537 GGATCC [GA]^GATC[CT] 1244 GGATCC MspA1I 677 CAGCTG C[AC]G^C[GT]G 768 CAGCTG NspI 396 ACATGT [GA]CATG^[CT] 1459 GCATGT PmaCI 59 CACGTG CAC^GTG 921 CACGTG PmlI 59 CACGTG CAC^GTG 921 CACGTG PvuII 677 CAGCTG CAG^CTG 768 CAGCTG SfaNI 956 GATGC GCATC(5/9) 1886 GCATC StyI 49 CCAAGG C^C[AT][AT]GG 253 CCTTGG XcmI 244 CCAGGGGCCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1196 CCACCTTTGCCCTGG XhoII 537 GGATCC [GA]^GATC[CT] 1244 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AciI AfaI AflIII AluI AlwI AvaII BbvI BcnI BfaI BmyI BpmI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfcI TaqI Tru9I TseI Tsp45I TspRI TthHB8I