##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AviII BbeI BbvCI BciVI BfrI
BglII BlpI Bpu1102I BsgI BsiWI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII Bst1107I BstBI
BstZ17I Bsu36I CelII Cfr10I ClaI
DraI Eam1105I Eco47III Eco81I EcoRV
FseI FspI HaeII HindIII HpaI
KasI KpnI MluI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI PvuII RcaI SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AatII 952 GACGTC GACGT^C
Asp700I 675 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1565 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1648 CTCGGG C^[CT]CG[GA]G
AvrII 59 CCTAGG C^CTAGG
BalI 1607 TGGCCA TGG^CCA
BamHI 224 GGATCC G^GATCC
BanI 1251 GGTGCC G^G[CT][GA]CC
BbrPI 1343 CACGTG CAC^GTG
BcgI 805 GCATGCTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1558 TGATCA T^GATCA
BfaI 60 CTAG C^TAG
BglI 825 GCCCGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 59 CCTAGG C^CTAGG
BsaAI 1343 CACGTG [CT]AC^GT[GA]
BsaBI 1631 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 322 CTCCTC GAGGAG(10/8)
BsiEI 815 CGGCCG CG[GA][CT]^CG
BsmI 996 GCATTC GAATGC(1/-1)
BsoBI 1648 CTCGGG C^[CT]CG[GA]G
BsrDI 1321 GCAATG GCAATG(2/0)
BstAPI 1471 GCAAGTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 106 GGTCACC G^GT[ACGT]ACC
BstPI 106 GGTCACC G^GT[ACGT]ACC
BstYI 224 GGATCC [GA]^GATC[CT]
DraIII 109 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 118 GACCGGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 815 CGGCCG C^GGCCG
EarI 741 CTCTTC CTCTTC(1/4)
EclXI 815 CGGCCG C^GGCCG
Eco52I 815 CGGCCG C^GGCCG
EcoO65I 106 GGTCACC G^GT[ACGT]ACC
EcoRI 1367 GAATTC G^AATTC
EcoT22I 994 ATGCAT ATGCA^T
FbaI 1558 TGATCA T^GATCA
HincII 774 GTTGAC GT[CT]^[GA]AC
HindII 774 GTTGAC GT[CT]^[GA]AC
Ksp632I 741 CTCTTC CTCTTC(1/4)
KspI 102 CCGCGG CCGC^GG
MaeI 60 CTAG C^TAG
MamI 1631 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1641 CAATTG C^AATTG
MflI 224 GGATCC [GA]^GATC[CT]
MluNI 1607 TGGCCA TGG^CCA
MscI 1607 TGGCCA TGG^CCA
MseI 189 TTAA T^TAA
MunI 1641 CAATTG C^AATTG
NcoI 271 CCATGG C^CATGG
NdeI 1490 CATATG CA^TATG
NsiI 994 ATGCAT ATGCA^T
PflFI 1565 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1013 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1459 GACTC GAGTC(4/5)
PmaCI 1343 CACGTG CAC^GTG
PmlI 1343 CACGTG CAC^GTG
PstI 628 CTGCAG CTGCA^G
SacII 102 CCGCGG CCGC^GG
SapI 740 GCTCTTC GCTCTTC(1/4)
Tru9I 189 TTAA T^TAA
Tth111I 1565 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1013 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 224 GGATCC [GA]^GATC[CT]
XmnI 675 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccIII 84 TCCGGA T^CCGGA
96 TCCGGA
AcsI 1301 GAATTT [GA]^AATT[CT]
1367 GAATTC
AfaI 593 GTAC GT^AC
856 GTAC
AflIII 1342 ACACGT A^C[GA][CT]GT
1344 ACGTGT
ApaI 965 GGGCCC GGGCC^C
1651 GGGCCC
ApoI 1301 GAATTT [GA]^AATT[CT]
1367 GAATTC
BpmI 1481 CTCCAG CTGGAG(16/14)
612 CTGGAG
BsaI 1695 GAGACC GGTCTC(1/5)
1062 GGTCTC
BsaWI 84 TCCGGA [AT]^CCGG[AT]
96 TCCGGA
BseAI 84 TCCGGA T^CCGGA
96 TCCGGA
BsmBI 923 GAGACG CGTCTC(1/5)
1286 GAGACG
BspEI 84 TCCGGA T^CCGGA
96 TCCGGA
BspMI 469 ACCTGC ACCTGC(4/8)
1467 ACCTGC
CpoI 81 CGGTCCG CG^G[AT]CCG
98 CGGACCG
DsaI 102 CCGCGG C^C[GA][CT]GG
271 CCATGG
EaeI 815 CGGCCG [CT]^GGCC[GA]
1607 TGGCCA
MroI 84 TCCGGA T^CCGGA
96 TCCGGA
MslI 519 CATTTAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
782 CATCCGTGTG
PshAI 100 GACCGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1341 GACACGTGTC
RsaI 593 GTAC GT^AC
856 GTAC
RsrII 81 CGGTCCG CG^G[AT]CCG
98 CGGACCG
SacI 408 GAGCTC GAGCT^C
582 GAGCTC
SphI 805 GCATGC GCATG^C
1258 GCATGC
XcmI 8 CCAACTACGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1234 CCACCGAGCCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI AspHI AvaII BanII BbsI
BbvI BcnI BmyI BpuAI BsaHI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PpuMI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I