##################################
# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII AccI AccII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI ApaI ApaLI AscI AseI
AsnI AspEI AspI AvaI AviII
BalI BamHI BbeI BbrPI BcgI
BclI BcnI BfrI BglI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DrdI EaeI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KspI MamI
MfeI MluI MluNI MscI MslI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 738 GGTACC G^GTACC
AccIII 22 TCCGGA T^CCGGA
AlwI 1102 GATCC GGATC(4/5)
AlwNI 1160 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 738 GGTACC G^GTACC
AvrII 1317 CCTAGG C^CTAGG
BanI 738 GGTACC G^G[CT][GA]CC
BbsI 770 GAAGAC GAAGAC(2/6)
BbvCI 553 GCTGAGG CCTCAGC(-5/-2)
BciVI 367 GTATCC GTATCC(6/5)
BglII 958 AGATCT A^GATCT
BlnI 1317 CCTAGG C^CTAGG
BpuAI 770 GAAGAC GAAGAC(2/6)
BsaWI 22 TCCGGA [AT]^CCGG[AT]
BseAI 22 TCCGGA T^CCGGA
BsgI 245 GTGCAG GTGCAG(16/14)
BsmFI 1022 GGGAC GGGAC(10/14)
BspEI 22 TCCGGA T^CCGGA
BsrDI 354 GCAATG GCAATG(2/0)
BssSI 1361 CACGAG CACGAG(-5/-1)
Cac8I 679 GCCCGC GC[ACGT]^[ACGT]GC
DraII 1161 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 399 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 674 CCATGG C^C[GA][CT]GG
EcoO109I 1161 AGGACCT [GA]G^G[ACGT]CC[CT]
FauI 680 CCCGC CCCGC(4/6)
HapII 23 CCGG C^CGG
HgaI 540 GACGC GACGC(5/10)
HpaII 23 CCGG C^CGG
KpnI 738 GGTACC GGTAC^C
MaeII 648 ACGT A^CGT
MroI 22 TCCGGA T^CCGGA
MspA1I 1012 CAGCGG C[AC]G^C[GT]G
MspI 23 CCGG C^CGG
NcoI 674 CCATGG C^CATGG
NlaIV 738 GGTACC GG[ACGT]^[ACGT]CC
PflMI 1159 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 988 GAGTC GAGTC(4/5)
PpuMI 1161 AGGACCT [GA]G^G[AT]CC[CT]
PstI 851 CTGCAG CTGCA^G
SapI 788 GCTCTTC GCTCTTC(1/4)
SmlI 632 CTCAAG C^T[CT][GA]AG
Van91I 1159 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 136 TCTAGA T^CTAGA
XcmI 1155 CCAACCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 4 GAATTT [GA]^AATT[CT]
224 GAATTT
Aor51HI 640 AGCGCT AGC^GCT
1328 AGCGCT
ApoI 4 GAATTT [GA]^AATT[CT]
224 GAATTT
Asp700I 298 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1257 GAAACCTTTC
BspMI 178 GCAGGT ACCTGC(4/8)
580 GCAGGT
BstYI 958 AGATCT [GA]^GATC[CT]
1101 AGATCC
CfoI 641 GCGC GCG^C
1329 GCGC
EarI 789 CTCTTC CTCTTC(1/4)
691 GAAGAG
Eco47III 640 AGCGCT AGC^GCT
1328 AGCGCT
EcoT14I 674 CCATGG C^C[AT][AT]GG
1317 CCTAGG
HaeII 640 AGCGCT [GA]GCGC^[CT]
1328 AGCGCT
HhaI 641 GCGC GCG^C
1329 GCGC
HinP1I 641 GCGC G^CGC
1329 GCGC
Ksp632I 789 CTCTTC CTCTTC(1/4)
691 GAAGAG
MflI 958 AGATCT [GA]^GATC[CT]
1101 AGATCC
SfcI 851 CTGCAG C^T[GA][CT]AG
1038 CTATAG
StuI 300 AGGCCT AGG^CCT
1034 AGGCCT
StyI 674 CCATGG C^C[AT][AT]GG
1317 CCTAGG
TaqI 938 TCGA T^CGA
1111 TCGA
Tsp45I 182 GTCAC ^GT[GC]AC
1344 GTGAC
Tsp509I 5 AATT ^AATT
225 AATT
TthHB8I 938 TCGA T^CGA
1111 TCGA
XhoII 958 AGATCT [GA]^GATC[CT]
1101 AGATCC
XmnI 298 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1257 GAAACCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BanII BbvI BfaI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeI MaeIII MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI TfiI Tru9I TseI
TspRI