################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII AccI AccII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI AspEI AspI AvaI AviII BalI BamHI BbeI BbrPI BcgI BclI BcnI BfrI BglI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DrdI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KspI MamI MfeI MluI MluNI MscI MslI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 738 GGTACC G^GTACC AccIII 22 TCCGGA T^CCGGA AlwI 1102 GATCC GGATC(4/5) AlwNI 1160 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG Asp718I 738 GGTACC G^GTACC AvrII 1317 CCTAGG C^CTAGG BanI 738 GGTACC G^G[CT][GA]CC BbsI 770 GAAGAC GAAGAC(2/6) BbvCI 553 GCTGAGG CCTCAGC(-5/-2) BciVI 367 GTATCC GTATCC(6/5) BglII 958 AGATCT A^GATCT BlnI 1317 CCTAGG C^CTAGG BpuAI 770 GAAGAC GAAGAC(2/6) BsaWI 22 TCCGGA [AT]^CCGG[AT] BseAI 22 TCCGGA T^CCGGA BsgI 245 GTGCAG GTGCAG(16/14) BsmFI 1022 GGGAC GGGAC(10/14) BspEI 22 TCCGGA T^CCGGA BsrDI 354 GCAATG GCAATG(2/0) BssSI 1361 CACGAG CACGAG(-5/-1) Cac8I 679 GCCCGC GC[ACGT]^[ACGT]GC DraII 1161 AGGACCT [GA]G^G[ACGT]CC[CT] DraIII 399 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 674 CCATGG C^C[GA][CT]GG EcoO109I 1161 AGGACCT [GA]G^G[ACGT]CC[CT] FauI 680 CCCGC CCCGC(4/6) HapII 23 CCGG C^CGG HgaI 540 GACGC GACGC(5/10) HpaII 23 CCGG C^CGG KpnI 738 GGTACC GGTAC^C MaeII 648 ACGT A^CGT MroI 22 TCCGGA T^CCGGA MspA1I 1012 CAGCGG C[AC]G^C[GT]G MspI 23 CCGG C^CGG NcoI 674 CCATGG C^CATGG NlaIV 738 GGTACC GG[ACGT]^[ACGT]CC PflMI 1159 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 988 GAGTC GAGTC(4/5) PpuMI 1161 AGGACCT [GA]G^G[AT]CC[CT] PstI 851 CTGCAG CTGCA^G SapI 788 GCTCTTC GCTCTTC(1/4) SmlI 632 CTCAAG C^T[CT][GA]AG Van91I 1159 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 136 TCTAGA T^CTAGA XcmI 1155 CCAACCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcsI 4 GAATTT [GA]^AATT[CT] 224 GAATTT Aor51HI 640 AGCGCT AGC^GCT 1328 AGCGCT ApoI 4 GAATTT [GA]^AATT[CT] 224 GAATTT Asp700I 298 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1257 GAAACCTTTC BspMI 178 GCAGGT ACCTGC(4/8) 580 GCAGGT BstYI 958 AGATCT [GA]^GATC[CT] 1101 AGATCC CfoI 641 GCGC GCG^C 1329 GCGC EarI 789 CTCTTC CTCTTC(1/4) 691 GAAGAG Eco47III 640 AGCGCT AGC^GCT 1328 AGCGCT EcoT14I 674 CCATGG C^C[AT][AT]GG 1317 CCTAGG HaeII 640 AGCGCT [GA]GCGC^[CT] 1328 AGCGCT HhaI 641 GCGC GCG^C 1329 GCGC HinP1I 641 GCGC G^CGC 1329 GCGC Ksp632I 789 CTCTTC CTCTTC(1/4) 691 GAAGAG MflI 958 AGATCT [GA]^GATC[CT] 1101 AGATCC SfcI 851 CTGCAG C^T[GA][CT]AG 1038 CTATAG StuI 300 AGGCCT AGG^CCT 1034 AGGCCT StyI 674 CCATGG C^C[AT][AT]GG 1317 CCTAGG TaqI 938 TCGA T^CGA 1111 TCGA Tsp45I 182 GTCAC ^GT[GC]AC 1344 GTGAC Tsp509I 5 AATT ^AATT 225 AATT TthHB8I 938 TCGA T^CGA 1111 TCGA XhoII 958 AGATCT [GA]^GATC[CT] 1101 AGATCC XmnI 298 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1257 GAAACCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AciI AfaI AluI AspHI AvaII BanII BbvI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII RsaI SacI Sau3AI Sau96I ScrFI SfaNI TfiI Tru9I TseI TspRI