##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
Acc65I AccI AccII AclI AflII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AviII
BalI BanI BbeI BciVI BclI
BfrI BglI BlpI Bpu1102I BsaBI
BseRI BsiEI BsiWI BspDI BspHI
BssHII BssSI Bst1107I BstBI BstUI
BstXI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI EaeI EagI
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MscI MunI MvnI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI Sse8387I SspI
StuI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 1183 GACGTC GACGT^C
AcsI 1547 GAATTT [GA]^AATT[CT]
AocI 1241 CCTCAGG CC^T[ACGT]AGG
ApoI 1547 GAATTT [GA]^AATT[CT]
AspI 743 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1596 CCTAGG C^CTAGG
BamHI 908 GGATCC G^GATCC
BanII 1325 GAGCTC G[GA]GC[CT]^C
BbrPI 854 CACGTG CAC^GTG
BcgI 956 CGACTAGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1596 CCTAGG C^CTAGG
BsaAI 854 CACGTG [CT]AC^GT[GA]
BsgI 866 GTGCAG GTGCAG(16/14)
BsmBI 794 GAGACG CGTCTC(1/5)
BsmI 590 GCATTC GAATGC(1/-1)
Bsp1407I 699 TGTACA T^GTACA
BspLU11I 696 ACATGT A^CATGT
BsrBI 575 GAGCGG CCGCTC(-3/-3)
BsrDI 1539 GCAATG GCAATG(2/0)
BsrGI 699 TGTACA T^GTACA
BstEII 1060 GGTCACC G^GT[ACGT]ACC
BstPI 1060 GGTCACC G^GT[ACGT]ACC
Bsu36I 1241 CCTCAGG CC^T[ACGT]AGG
DsaI 504 CCATGG C^C[GA][CT]GG
Eco81I 1241 CCTCAGG CC^T[ACGT]AGG
EcoNI 1378 CCTCAAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1060 GGTCACC G^GT[ACGT]ACC
HaeII 1460 AGCGCC [GA]GCGC^[CT]
MseI 1044 TTAA T^TAA
NaeI 972 GCCGGC GCC^GGC
NcoI 504 CCATGG C^CATGG
NgoMIV 972 GCCGGC G^CCGGC
PaeR7I 1328 CTCGAG C^TCGAG
PflFI 743 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1006 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 854 CACGTG CAC^GTG
PmlI 854 CACGTG CAC^GTG
PvuII 235 CAGCTG CAG^CTG
SacI 1325 GAGCTC GAGCT^C
SapI 1144 GCTCTTC GCTCTTC(1/4)
ScaI 1158 AGTACT AGT^ACT
SmaI 789 CCCGGG CCC^GGG
SpeI 958 ACTAGT A^CTAGT
SphI 1131 GCATGC GCATG^C
SspBI 699 TGTACA T^GTACA
TfiI 819 GAATC G^A[AT]TC
Tru9I 1044 TTAA T^TAA
Tth111I 743 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1006 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 654 CCATCTTCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1328 CTCGAG C^TCGAG
XmaCI 789 CCCGGG C^CCGGG
XmaI 789 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 525 TCCGGA T^CCGGA
639 TCCGGA
AcyI 1183 GACGTC G[GA]^CG[CT]C
1509 GGCGTC
AflIII 696 ACATGT A^C[GA][CT]GT
855 ACGTGT
AhdI 851 GACCACGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
954 GACGACTAGTC
AlwNI 1007 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1136 CAGGTCCTG
AspEI 851 GACCACGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
954 GACGACTAGTC
BbsI 779 GAAGAC GAAGAC(2/6)
838 GAAGAC
BbvCI 406 CCTCAGC CCTCAGC(-5/-2)
1465 CCTCAGC
BcnI 789 CCCGG CC^[GC]GG
790 CCGGG
BglII 1032 AGATCT A^GATCT
1068 AGATCT
BpuAI 779 GAAGAC GAAGAC(2/6)
838 GAAGAC
BsaHI 1183 GACGTC G[GA]^CG[CT]C
1509 GGCGTC
BsaI 224 GAGACC GGTCTC(1/5)
1482 GAGACC
BseAI 525 TCCGGA T^CCGGA
639 TCCGGA
BspEI 525 TCCGGA T^CCGGA
639 TCCGGA
BspMI 1269 ACCTGC ACCTGC(4/8)
1135 GCAGGT
BstAPI 1135 GCAGGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1410 GCAGGGCTTGC
Eam1105I 851 GACCACGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
954 GACGACTAGTC
HgaI 796 GACGC GACGC(5/10)
1510 GCGTC
Hin1I 1183 GACGTC G[GA]^CG[CT]C
1509 GGCGTC
MaeII 855 ACGT A^CGT
1184 ACGT
MroI 525 TCCGGA T^CCGGA
639 TCCGGA
MspA1I 235 CAGCTG C[AC]G^C[GT]G
599 CAGCGG
NciI 789 CCCGG CC^[GC]GG
790 CCGGG
PpuMI 1137 AGGTCCT [GA]G^G[AT]CC[CT]
1599 AGGACCC
PstI 154 CTGCAG CTGCA^G
392 CTGCAG
SfcI 154 CTGCAG C^T[GA][CT]AG
392 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AfaI AluI AlwI Asp700I
AspHI AvaI AvaII BbvI BfaI
BmyI BpmI BsaJI BsaWI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI