################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## Acc65I AccI AccII AclI AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AviII BalI BanI BbeI BciVI BclI BfrI BglI BlpI Bpu1102I BsaBI BseRI BsiEI BsiWI BspDI BspHI BssHII BssSI Bst1107I BstBI BstUI BstXI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EaeI EagI EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MscI MunI MvnI NarI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI Sse8387I SspI StuI SwaI XbaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AatII 1183 GACGTC GACGT^C AcsI 1547 GAATTT [GA]^AATT[CT] AocI 1241 CCTCAGG CC^T[ACGT]AGG ApoI 1547 GAATTT [GA]^AATT[CT] AspI 743 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 1596 CCTAGG C^CTAGG BamHI 908 GGATCC G^GATCC BanII 1325 GAGCTC G[GA]GC[CT]^C BbrPI 854 CACGTG CAC^GTG BcgI 956 CGACTAGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 1596 CCTAGG C^CTAGG BsaAI 854 CACGTG [CT]AC^GT[GA] BsgI 866 GTGCAG GTGCAG(16/14) BsmBI 794 GAGACG CGTCTC(1/5) BsmI 590 GCATTC GAATGC(1/-1) Bsp1407I 699 TGTACA T^GTACA BspLU11I 696 ACATGT A^CATGT BsrBI 575 GAGCGG CCGCTC(-3/-3) BsrDI 1539 GCAATG GCAATG(2/0) BsrGI 699 TGTACA T^GTACA BstEII 1060 GGTCACC G^GT[ACGT]ACC BstPI 1060 GGTCACC G^GT[ACGT]ACC Bsu36I 1241 CCTCAGG CC^T[ACGT]AGG DsaI 504 CCATGG C^C[GA][CT]GG Eco81I 1241 CCTCAGG CC^T[ACGT]AGG EcoNI 1378 CCTCAAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1060 GGTCACC G^GT[ACGT]ACC HaeII 1460 AGCGCC [GA]GCGC^[CT] MseI 1044 TTAA T^TAA NaeI 972 GCCGGC GCC^GGC NcoI 504 CCATGG C^CATGG NgoMIV 972 GCCGGC G^CCGGC PaeR7I 1328 CTCGAG C^TCGAG PflFI 743 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1006 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 854 CACGTG CAC^GTG PmlI 854 CACGTG CAC^GTG PvuII 235 CAGCTG CAG^CTG SacI 1325 GAGCTC GAGCT^C SapI 1144 GCTCTTC GCTCTTC(1/4) ScaI 1158 AGTACT AGT^ACT SmaI 789 CCCGGG CCC^GGG SpeI 958 ACTAGT A^CTAGT SphI 1131 GCATGC GCATG^C SspBI 699 TGTACA T^GTACA TfiI 819 GAATC G^A[AT]TC Tru9I 1044 TTAA T^TAA Tth111I 743 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1006 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 654 CCATCTTCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1328 CTCGAG C^TCGAG XmaCI 789 CCCGGG C^CCGGG XmaI 789 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccIII 525 TCCGGA T^CCGGA 639 TCCGGA AcyI 1183 GACGTC G[GA]^CG[CT]C 1509 GGCGTC AflIII 696 ACATGT A^C[GA][CT]GT 855 ACGTGT AhdI 851 GACCACGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 954 GACGACTAGTC AlwNI 1007 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG 1136 CAGGTCCTG AspEI 851 GACCACGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 954 GACGACTAGTC BbsI 779 GAAGAC GAAGAC(2/6) 838 GAAGAC BbvCI 406 CCTCAGC CCTCAGC(-5/-2) 1465 CCTCAGC BcnI 789 CCCGG CC^[GC]GG 790 CCGGG BglII 1032 AGATCT A^GATCT 1068 AGATCT BpuAI 779 GAAGAC GAAGAC(2/6) 838 GAAGAC BsaHI 1183 GACGTC G[GA]^CG[CT]C 1509 GGCGTC BsaI 224 GAGACC GGTCTC(1/5) 1482 GAGACC BseAI 525 TCCGGA T^CCGGA 639 TCCGGA BspEI 525 TCCGGA T^CCGGA 639 TCCGGA BspMI 1269 ACCTGC ACCTGC(4/8) 1135 GCAGGT BstAPI 1135 GCAGGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1410 GCAGGGCTTGC Eam1105I 851 GACCACGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 954 GACGACTAGTC HgaI 796 GACGC GACGC(5/10) 1510 GCGTC Hin1I 1183 GACGTC G[GA]^CG[CT]C 1509 GGCGTC MaeII 855 ACGT A^CGT 1184 ACGT MroI 525 TCCGGA T^CCGGA 639 TCCGGA MspA1I 235 CAGCTG C[AC]G^C[GT]G 599 CAGCGG NciI 789 CCCGG CC^[GC]GG 790 CCGGG PpuMI 1137 AGGTCCT [GA]G^G[AT]CC[CT] 1599 AGGACCC PstI 154 CTGCAG CTGCA^G 392 CTGCAG SfcI 154 CTGCAG C^T[GA][CT]AG 392 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AciI AfaI AluI AlwI Asp700I AspHI AvaI AvaII BbvI BfaI BmyI BpmI BsaJI BsaWI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MslI MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI