##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

Acc65I      AccI        AccII       AclI        AflII       
AgeI        Alw44I      Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AviII       
BalI        BanI        BbeI        BciVI       BclI        
BfrI        BglI        BlpI        Bpu1102I    BsaBI       
BseRI       BsiEI       BsiWI       BspDI       BspHI       
BssHII      BssSI       Bst1107I    BstBI       BstUI       
BstXI       BstZ17I     CelII       ClaI        CpoI        
DraI        DraIII      DrdI        EaeI        EagI        
EclXI       Eco47III    Eco52I      EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MluNI       
MscI        MunI        MvnI        NarI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       Sse8387I    SspI        
StuI        SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AatII        1183  GACGTC               GACGT^C
AcsI         1547  GAATTT               [GA]^AATT[CT]
AocI         1241  CCTCAGG              CC^T[ACGT]AGG
ApoI         1547  GAATTT               [GA]^AATT[CT]
AspI          743  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        1596  CCTAGG               C^CTAGG
BamHI         908  GGATCC               G^GATCC
BanII        1325  GAGCTC               G[GA]GC[CT]^C
BbrPI         854  CACGTG               CAC^GTG
BcgI          956  CGACTAGTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI         1596  CCTAGG               C^CTAGG
BsaAI         854  CACGTG               [CT]AC^GT[GA]
BsgI          866  GTGCAG               GTGCAG(16/14)
BsmBI         794  GAGACG               CGTCTC(1/5)
BsmI          590  GCATTC               GAATGC(1/-1)
Bsp1407I      699  TGTACA               T^GTACA
BspLU11I      696  ACATGT               A^CATGT
BsrBI         575  GAGCGG               CCGCTC(-3/-3)
BsrDI        1539  GCAATG               GCAATG(2/0)
BsrGI         699  TGTACA               T^GTACA
BstEII       1060  GGTCACC              G^GT[ACGT]ACC
BstPI        1060  GGTCACC              G^GT[ACGT]ACC
Bsu36I       1241  CCTCAGG              CC^T[ACGT]AGG
DsaI          504  CCATGG               C^C[GA][CT]GG
Eco81I       1241  CCTCAGG              CC^T[ACGT]AGG
EcoNI        1378  CCTCAAAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1060  GGTCACC              G^GT[ACGT]ACC
HaeII        1460  AGCGCC               [GA]GCGC^[CT]
MseI         1044  TTAA                 T^TAA
NaeI          972  GCCGGC               GCC^GGC
NcoI          504  CCATGG               C^CATGG
NgoMIV        972  GCCGGC               G^CCGGC
PaeR7I       1328  CTCGAG               C^TCGAG
PflFI         743  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1006  CCAGAAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         854  CACGTG               CAC^GTG
PmlI          854  CACGTG               CAC^GTG
PvuII         235  CAGCTG               CAG^CTG
SacI         1325  GAGCTC               GAGCT^C
SapI         1144  GCTCTTC              GCTCTTC(1/4)
ScaI         1158  AGTACT               AGT^ACT
SmaI          789  CCCGGG               CCC^GGG
SpeI          958  ACTAGT               A^CTAGT
SphI         1131  GCATGC               GCATG^C
SspBI         699  TGTACA               T^GTACA
TfiI          819  GAATC                G^A[AT]TC
Tru9I        1044  TTAA                 T^TAA
Tth111I       743  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1006  CCAGAAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          654  CCATCTTCATCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1328  CTCGAG               C^TCGAG
XmaCI         789  CCCGGG               C^CCGGG
XmaI          789  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccIII        525  TCCGGA               T^CCGGA
              639  TCCGGA              
AcyI         1183  GACGTC               G[GA]^CG[CT]C
             1509  GGCGTC              
AflIII        696  ACATGT               A^C[GA][CT]GT
              855  ACGTGT              
AhdI          851  GACCACGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              954  GACGACTAGTC         
AlwNI        1007  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1136  CAGGTCCTG           
AspEI         851  GACCACGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              954  GACGACTAGTC         
BbsI          779  GAAGAC               GAAGAC(2/6)
              838  GAAGAC              
BbvCI         406  CCTCAGC              CCTCAGC(-5/-2)
             1465  CCTCAGC             
BcnI          789  CCCGG                CC^[GC]GG
              790  CCGGG               
BglII        1032  AGATCT               A^GATCT
             1068  AGATCT              
BpuAI         779  GAAGAC               GAAGAC(2/6)
              838  GAAGAC              
BsaHI        1183  GACGTC               G[GA]^CG[CT]C
             1509  GGCGTC              
BsaI          224  GAGACC               GGTCTC(1/5)
             1482  GAGACC              
BseAI         525  TCCGGA               T^CCGGA
              639  TCCGGA              
BspEI         525  TCCGGA               T^CCGGA
              639  TCCGGA              
BspMI        1269  ACCTGC               ACCTGC(4/8)
             1135  GCAGGT              
BstAPI       1135  GCAGGTCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1410  GCAGGGCTTGC         
Eam1105I      851  GACCACGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              954  GACGACTAGTC         
HgaI          796  GACGC                GACGC(5/10)
             1510  GCGTC               
Hin1I        1183  GACGTC               G[GA]^CG[CT]C
             1509  GGCGTC              
MaeII         855  ACGT                 A^CGT
             1184  ACGT                
MroI          525  TCCGGA               T^CCGGA
              639  TCCGGA              
MspA1I        235  CAGCTG               C[AC]G^C[GT]G
              599  CAGCGG              
NciI          789  CCCGG                CC^[GC]GG
              790  CCGGG               
PpuMI        1137  AGGTCCT              [GA]G^G[AT]CC[CT]
             1599  AGGACCC             
PstI          154  CTGCAG               CTGCA^G
              392  CTGCAG              
SfcI          154  CTGCAG               C^T[GA][CT]AG
              392  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (   80 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        Asp700I     
AspHI       AvaI        AvaII       BbvI        BfaI        
BmyI        BpmI        BsaJI       BsaWI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       EarI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MflI        
MnlI        MslI        MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       NspI        PleI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SmlI        StyI        TaqI        TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       XmnI