##################################
# NO CUT ENZYMES ( 175 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DpnII DraI
DraIII DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoT14I EcoT22I FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaI
KasI KpnI Ksp632I KspI MaeII
MaeIII MamI MboI MfeI MflI
MluI MroI MseI MslI MunI
NaeI NarI NcoI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
Sau3AI SbfI SfaNI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI StyI
SwaI TaqI TfiI Tru9I Tsp45I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccII 379 CGCG CG^CG
AcsI 42 GAATTC [GA]^AATT[CT]
AlwNI 458 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 42 GAATTC [GA]^AATT[CT]
AspHI 258 GAGCTC G[AT]GC[AT]^C
AvaII 56 GGACC G^G[AT]CC
BfaI 306 CTAG C^TAG
BglI 402 GCCCAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 111 CTGGAG CTGGAG(16/14)
BsiHKAI 258 GAGCTC G[AT]GC[AT]^C
BsmAI 330 GTCTC GTCTC(1/5)
Bsp1407I 370 TGTACA T^GTACA
BsrGI 370 TGTACA T^GTACA
BstAPI 457 GCAGCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 379 CGCG CG^CG
DraII 55 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoNI 32 CCTCATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 55 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 42 GAATTC G^AATTC
EcoRV 63 GATATC GAT^ATC
FauI 377 CCCGC CCCGC(4/6)
HgaI 380 GCGTC GACGC(5/10)
MaeI 306 CTAG C^TAG
MspA1I 108 CAGCTG C[AC]G^C[GT]G
MvnI 379 CGCG CG^CG
PpuMI 55 GGGACCC [GA]G^G[AT]CC[CT]
PstI 297 CTGCAG CTGCA^G
PvuII 108 CAGCTG CAG^CTG
SacI 258 GAGCTC GAGCT^C
ScaI 238 AGTACT AGT^ACT
SexAI 204 ACCAGGT A^CC[AT]GGT
SfcI 297 CTGCAG C^T[GA][CT]AG
SfiI 401 GGCCCAGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 370 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 239 GTAC GT^AC
371 GTAC
BalI 409 TGGCCA TGG^CCA
439 TGGCCA
BanII 114 GAGCCC G[GA]GC[CT]^C
258 GAGCTC
BbsI 90 GAAGAC GAAGAC(2/6)
73 GTCTTC
BcnI 59 CCCGG CC^[GC]GG
269 CCGGG
BmyI 114 GAGCCC G[AGT]GC[ACT]^C
258 GAGCTC
BpuAI 90 GAAGAC GAAGAC(2/6)
73 GTCTTC
BsaJI 249 CCTGGG C^C[ACGT][ACGT]GG
424 CCGAGG
BseRI 261 CTCCTC GAGGAG(10/8)
155 GAGGAG
BsmFI 55 GGGAC GGGAC(10/14)
271 GGGAC
Bsp1286I 114 GAGCCC G[AGT]GC[ACT]^C
258 GAGCTC
BstXI 226 CCAGCCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
412 CCATTCTTCTGG
Cac8I 225 GCCAGC GC[ACGT]^[ACGT]GC
398 GCTGGC
DdeI 284 CTTAG C^T[ACGT]AG
332 CTCAG
HphI 387 GGTGA GGTGA(8/7)
139 TCACC
MluNI 409 TGGCCA TGG^CCA
439 TGGCCA
MscI 409 TGGCCA TGG^CCA
439 TGGCCA
NciI 59 CCCGG CC^[GC]GG
269 CCGGG
NlaIII 35 CATG CATG^
363 CATG
RsaI 239 GTAC GT^AC
371 GTAC
Tsp509I 15 AATT ^AATT
43 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AluI BbvI BsiYI BslI
BsrI BssKI BstF5I BstNI Cfr13I
EaeI EcoRII Fnu4HI FokI HaeIII
HapII HpaII ItaI MboII MnlI
MspI MvaI MwoI NlaIV Sau96I
ScrFI TseI TspRI