##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AgeI AhdI AlwI Aor51HI ApaI
ApoI AscI Asp718I AspEI AspI
AvrII BamHI BbrPI BcgI BciVI
BclI BfrI BlnI BsaAI BsaBI
BsaI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BsrFI BsrGI BssHII
Bst1107I BstBI BstXI BstZ17I Cfr10I
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KpnI MamI
MfeI MluI MunI NaeI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SalI ScaI SexAI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI StuI SwaI TaqI Tsp509I
Tth111I TthHB8I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 1029 GACGTC GACGT^C
AccIII 143 TCCGGA T^CCGGA
AflIII 1523 ACATGT A^C[GA][CT]GT
AseI 631 ATTAAT AT^TAAT
AsnI 631 ATTAAT AT^TAAT
AviII 983 TGCGCA TGC^GCA
BalI 8 TGGCCA TGG^CCA
BbeI 977 GGCGCC GGCGC^C
BbvCI 1386 GCTGAGG CCTCAGC(-5/-2)
BsaWI 143 TCCGGA [AT]^CCGG[AT]
BseAI 143 TCCGGA T^CCGGA
BsgI 439 CTGCAC GTGCAG(16/14)
BsmAI 1312 GAGAC GTCTC(1/5)
BspEI 143 TCCGGA T^CCGGA
BspHI 900 TCATGA T^CATGA
BspLU11I 1523 ACATGT A^CATGT
BspMI 179 ACCTGC ACCTGC(4/8)
BsrDI 33 CATTGC GCAATG(2/0)
BssSI 1552 CACGAG CACGAG(-5/-1)
BstEII 805 GGTGACC G^GT[ACGT]ACC
BstPI 805 GGTGACC G^GT[ACGT]ACC
DrdI 1029 GACGTCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 981 CCTGCGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 805 GGTGACC G^GT[ACGT]ACC
FspI 983 TGCGCA TGC^GCA
KasI 977 GGCGCC G^GCGCC
MaeII 1030 ACGT A^CGT
MluNI 8 TGGCCA TGG^CCA
MroI 143 TCCGGA T^CCGGA
MscI 8 TGGCCA TGG^CCA
MseI 632 TTAA T^TAA
NarI 977 GGCGCC GG^CGCC
NheI 1239 GCTAGC G^CTAGC
NspI 1523 ACATGT [GA]CATG^[CT]
PflMI 589 CCAGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 631 ATTAAT AT^TAAT
RcaI 900 TCATGA T^CATGA
SacI 787 GAGCTC GAGCT^C
SapI 784 GAAGAGC GCTCTTC(1/4)
SbfI 1269 CCTGCAGG CCTGCA^GG
SfiI 483 GGCCAGTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 977 GGCGCC GGC^GCC
SmaI 113 CCCGGG CCC^GGG
SmlI 790 CTCAAG C^T[CT][GA]AG
Sse8387I 1269 CCTGCAGG CCTGCA^GG
TfiI 329 GAATC G^A[AT]TC
Tru9I 632 TTAA T^TAA
Van91I 589 CCAGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 383 CCATGCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 113 CCCGGG C^CCGGG
XmaI 113 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 1107 CGCG CG^CG
1286 CGCG
AcyI 977 GGCGCC G[GA]^CG[CT]C
1029 GACGTC
AfaI 593 GTAC GT^AC
1527 GTAC
Alw44I 497 GTGCAC G^TGCAC
1261 GTGCAC
AocI 17 CCTCAGG CC^T[ACGT]AGG
772 CCTGAGG
ApaLI 497 GTGCAC G^TGCAC
1261 GTGCAC
BanI 977 GGCGCC G^G[CT][GA]CC
1281 GGCACC
BbsI 1101 GAAGAC GAAGAC(2/6)
1188 GAAGAC
BglII 1175 AGATCT A^GATCT
1613 AGATCT
BpmI 1587 CTGGAG CTGGAG(16/14)
1600 CTGGAG
BpuAI 1101 GAAGAC GAAGAC(2/6)
1188 GAAGAC
BsaHI 977 GGCGCC G[GA]^CG[CT]C
1029 GACGTC
BsmFI 797 GGGAC GGGAC(10/14)
1592 GTCCC
BsrBI 304 GAGCGG CCGCTC(-3/-3)
1408 GAGCGG
BstAPI 1263 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1428 GCAGGCTCTGC
BstUI 1107 CGCG CG^CG
1286 CGCG
BstYI 1175 AGATCT [GA]^GATC[CT]
1613 AGATCT
Bsu36I 17 CCTCAGG CC^T[ACGT]AGG
772 CCTGAGG
Eco81I 17 CCTCAGG CC^T[ACGT]AGG
772 CCTGAGG
HaeII 848 AGCGCC [GA]GCGC^[CT]
977 GGCGCC
Hin1I 977 GGCGCC G[GA]^CG[CT]C
1029 GACGTC
KspI 1106 CCGCGG CCGC^GG
1285 CCGCGG
MflI 1175 AGATCT [GA]^GATC[CT]
1613 AGATCT
MslI 33 CATTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1518 CATGGACATG
MvnI 1107 CGCG CG^CG
1286 CGCG
NcoI 38 CCATGG C^CATGG
1043 CCATGG
PpuMI 797 GGGACCT [GA]G^G[AT]CC[CT]
1036 GGGTCCT
PstI 946 CTGCAG CTGCA^G
1270 CTGCAG
RsaI 593 GTAC GT^AC
1527 GTAC
SacII 1106 CCGCGG CCGC^GG
1285 CCGCGG
SfaNI 183 GCATC GCATC(5/9)
264 GCATC
SfcI 946 CTGCAG C^T[GA][CT]AG
1270 CTGCAG
XhoII 1175 AGATCT [GA]^GATC[CT]
1613 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AluI AlwNI Asp700I AspHI
AvaI AvaII BanII BbvI BcnI
BfaI BglI BlpI BmyI Bpu1102I
BsaJI BseRI BsiHKAI BsiYI BslI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PvuII Sau3AI Sau96I ScrFI
StyI TseI Tsp45I TspRI XmnI