##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflIII AgeI ApaI AscI
AseI AsnI Asp700I Asp718I AvaI
AviII AvrII BalI BanI BanII
BbeI BbrPI BbvCI BclI BglI
BglII BlnI BsaAI BsaBI BsaHI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I ClaI CpoI DraI DraII
DraIII EagI EclXI Eco52I EcoNI
EcoO109I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HindIII
HpaI KasI KpnI KspI MamI
MluI MluNI MscI MspA1I MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AccIII 257 TCCGGA T^CCGGA
AcsI 39 GAATTC [GA]^AATT[CT]
AflII 798 CTTAAG C^TTAAG
AhdI 869 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 793 GTGCAC G^TGCAC
AlwNI 606 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 634 AGCGCT AGC^GCT
ApaLI 793 GTGCAC G^TGCAC
ApoI 39 GAATTC [GA]^AATT[CT]
AspEI 869 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 793 GTGCAC G[AT]GC[AT]^C
AspI 279 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 31 GGATCC G^GATCC
BbsI 276 GAAGAC GAAGAC(2/6)
BciVI 112 GTATCC GTATCC(6/5)
BcnI 1033 CCCGG CC^[GC]GG
BfaI 441 CTAG C^TAG
BfrI 798 CTTAAG C^TTAAG
BlpI 777 GCTAAGC GC^T[ACGT]AGC
BmyI 793 GTGCAC G[AGT]GC[ACT]^C
BpmI 1005 CTCCAG CTGGAG(16/14)
Bpu1102I 777 GCTAAGC GC^T[ACGT]AGC
BpuAI 276 GAAGAC GAAGAC(2/6)
BsaI 97 GGTCTC GGTCTC(1/5)
BsaWI 257 TCCGGA [AT]^CCGG[AT]
BseAI 257 TCCGGA T^CCGGA
BsgI 1182 CTGCAC GTGCAG(16/14)
BsiHKAI 793 GTGCAC G[AT]GC[AT]^C
BsmI 773 GAATGC GAATGC(1/-1)
Bsp1286I 793 GTGCAC G[AGT]GC[ACT]^C
BspEI 257 TCCGGA T^CCGGA
BsrFI 563 GCCGGT [GA]^CCGG[CT]
BstYI 31 GGATCC [GA]^GATC[CT]
CelII 777 GCTAAGC GC^T[ACGT]AGC
Cfr10I 563 GCCGGT [GA]^CCGG[CT]
DrdI 558 GACCAGCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1035 CGGCCA [CT]^GGCC[GA]
Eam1105I 869 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 634 AGCGCT AGC^GCT
EcoRI 39 GAATTC G^AATTC
EcoT14I 496 CCATGG C^C[AT][AT]GG
FauI 1093 CCCGC CCCGC(4/6)
HincII 1115 GTTGAC GT[CT]^[GA]AC
HindII 1115 GTTGAC GT[CT]^[GA]AC
MaeI 441 CTAG C^TAG
MfeI 358 CAATTG C^AATTG
MflI 31 GGATCC [GA]^GATC[CT]
MroI 257 TCCGGA T^CCGGA
MseI 799 TTAA T^TAA
MslI 86 CATGCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 358 CAATTG C^AATTG
NciI 1033 CCCGG CC^[GC]GG
NcoI 496 CCATGG C^CATGG
PflFI 279 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SfaNI 120 GATGC GCATC(5/9)
SphI 85 GCATGC GCATG^C
StyI 496 CCATGG C^C[AT][AT]GG
TaqI 43 TCGA T^CGA
Tru9I 799 TTAA T^TAA
Tth111I 279 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 43 TCGA T^CGA
XhoII 31 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AfaI 62 GTAC GT^AC
602 GTAC
BcgI 44 CGATCCAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1010 GCAGCAGGCTCG
BsmAI 98 GTCTC GTCTC(1/5)
693 GTCTC
BsmFI 224 GTCCC GGGAC(10/14)
918 GTCCC
BsrDI 146 CATTGC GCAATG(2/0)
923 CATTGC
BsrI 734 ACTGG ACTGG(1/-1)
1110 CCAGT
DsaI 496 CCATGG C^C[GA][CT]GG
1120 CCACGG
MaeII 478 ACGT A^CGT
682 ACGT
PstI 612 CTGCAG CTGCA^G
698 CTGCAG
RsaI 62 GTAC GT^AC
602 GTAC
SapI 317 GAAGAGC GCTCTTC(1/4)
981 GCTCTTC
SfcI 612 CTGCAG C^T[GA][CT]AG
698 CTGCAG
Tsp45I 463 GTGAC ^GT[GC]AC
514 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI AlwI AocI AvaII
BbvI BsaJI BseRI BsiYI BslI
BssKI BstF5I BstNI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII EarI
Eco81I EcoRII Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI Sau3AI Sau96I ScrFI SmlI
TfiI TseI Tsp509I TspRI