################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AflIII AgeI ApaI AscI AseI AsnI Asp700I Asp718I AvaI AviII AvrII BalI BanI BanII BbeI BbrPI BbvCI BclI BglI BglII BlnI BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I ClaI CpoI DraI DraII DraIII EagI EclXI Eco52I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI KspI MamI MluI MluNI MscI MspA1I MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AccIII 257 TCCGGA T^CCGGA AcsI 39 GAATTC [GA]^AATT[CT] AflII 798 CTTAAG C^TTAAG AhdI 869 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 793 GTGCAC G^TGCAC AlwNI 606 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 634 AGCGCT AGC^GCT ApaLI 793 GTGCAC G^TGCAC ApoI 39 GAATTC [GA]^AATT[CT] AspEI 869 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 793 GTGCAC G[AT]GC[AT]^C AspI 279 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 31 GGATCC G^GATCC BbsI 276 GAAGAC GAAGAC(2/6) BciVI 112 GTATCC GTATCC(6/5) BcnI 1033 CCCGG CC^[GC]GG BfaI 441 CTAG C^TAG BfrI 798 CTTAAG C^TTAAG BlpI 777 GCTAAGC GC^T[ACGT]AGC BmyI 793 GTGCAC G[AGT]GC[ACT]^C BpmI 1005 CTCCAG CTGGAG(16/14) Bpu1102I 777 GCTAAGC GC^T[ACGT]AGC BpuAI 276 GAAGAC GAAGAC(2/6) BsaI 97 GGTCTC GGTCTC(1/5) BsaWI 257 TCCGGA [AT]^CCGG[AT] BseAI 257 TCCGGA T^CCGGA BsgI 1182 CTGCAC GTGCAG(16/14) BsiHKAI 793 GTGCAC G[AT]GC[AT]^C BsmI 773 GAATGC GAATGC(1/-1) Bsp1286I 793 GTGCAC G[AGT]GC[ACT]^C BspEI 257 TCCGGA T^CCGGA BsrFI 563 GCCGGT [GA]^CCGG[CT] BstYI 31 GGATCC [GA]^GATC[CT] CelII 777 GCTAAGC GC^T[ACGT]AGC Cfr10I 563 GCCGGT [GA]^CCGG[CT] DrdI 558 GACCAGCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 1035 CGGCCA [CT]^GGCC[GA] Eam1105I 869 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 634 AGCGCT AGC^GCT EcoRI 39 GAATTC G^AATTC EcoT14I 496 CCATGG C^C[AT][AT]GG FauI 1093 CCCGC CCCGC(4/6) HincII 1115 GTTGAC GT[CT]^[GA]AC HindII 1115 GTTGAC GT[CT]^[GA]AC MaeI 441 CTAG C^TAG MfeI 358 CAATTG C^AATTG MflI 31 GGATCC [GA]^GATC[CT] MroI 257 TCCGGA T^CCGGA MseI 799 TTAA T^TAA MslI 86 CATGCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 358 CAATTG C^AATTG NciI 1033 CCCGG CC^[GC]GG NcoI 496 CCATGG C^CATGG PflFI 279 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC SfaNI 120 GATGC GCATC(5/9) SphI 85 GCATGC GCATG^C StyI 496 CCATGG C^C[AT][AT]GG TaqI 43 TCGA T^CGA Tru9I 799 TTAA T^TAA Tth111I 279 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 43 TCGA T^CGA XhoII 31 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AfaI 62 GTAC GT^AC 602 GTAC BcgI 44 CGATCCAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1010 GCAGCAGGCTCG BsmAI 98 GTCTC GTCTC(1/5) 693 GTCTC BsmFI 224 GTCCC GGGAC(10/14) 918 GTCCC BsrDI 146 CATTGC GCAATG(2/0) 923 CATTGC BsrI 734 ACTGG ACTGG(1/-1) 1110 CCAGT DsaI 496 CCATGG C^C[GA][CT]GG 1120 CCACGG MaeII 478 ACGT A^CGT 682 ACGT PstI 612 CTGCAG CTGCA^G 698 CTGCAG RsaI 62 GTAC GT^AC 602 GTAC SapI 317 GAAGAGC GCTCTTC(1/4) 981 GCTCTTC SfcI 612 CTGCAG C^T[GA][CT]AG 698 CTGCAG Tsp45I 463 GTGAC ^GT[GC]AC 514 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AluI AlwI AocI AvaII BbvI BsaJI BseRI BsiYI BslI BssKI BstF5I BstNI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII EarI Eco81I EcoRII Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI Sau3AI Sau96I ScrFI SmlI TfiI TseI Tsp509I TspRI