##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AgeI AhdI Alw44I AlwI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AvrII
BamHI BbsI BcgI BclI BfaI
BfrI BglI BlnI BpuAI BsaBI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BstBI BstXI ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KpnI KspI MaeI MamI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SapI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI SspBI SspI SwaI XbaI
XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccI 207 GTATAC GT^[AC][GT]AC
AccII 496 CGCG CG^CG
AccIII 371 TCCGGA T^CCGGA
AspI 548 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1517 TGCGCA TGC^GCA
BalI 92 TGGCCA TGG^CCA
BanI 1553 GGCGCC G^G[CT][GA]CC
BbeI 1553 GGCGCC GGCGC^C
BbrPI 1486 CACGTG CAC^GTG
BbvCI 212 CCTCAGC CCTCAGC(-5/-2)
BciVI 558 GGATAC GTATCC(6/5)
BglII 417 AGATCT A^GATCT
BsaAI 1486 CACGTG [CT]AC^GT[GA]
BseAI 371 TCCGGA T^CCGGA
BsgI 118 GTGCAG GTGCAG(16/14)
BsmBI 820 GAGACG CGTCTC(1/5)
BspEI 371 TCCGGA T^CCGGA
BssSI 830 CTCGTG CACGAG(-5/-1)
Bst1107I 207 GTATAC GTA^TAC
BstAPI 1482 GCAGCACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 496 CGCG CG^CG
BstYI 417 AGATCT [GA]^GATC[CT]
BstZ17I 207 GTATAC GTA^TAC
DraIII 712 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
FspI 1517 TGCGCA TGC^GCA
KasI 1553 GGCGCC G^GCGCC
MaeII 1487 ACGT A^CGT
MflI 417 AGATCT [GA]^GATC[CT]
MluNI 92 TGGCCA TGG^CCA
MroI 371 TCCGGA T^CCGGA
MscI 92 TGGCCA TGG^CCA
MslI 580 CACACAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 496 CGCG CG^CG
NaeI 1556 GCCGGC GCC^GGC
NarI 1553 GGCGCC GG^CGCC
NcoI 1337 CCATGG C^CATGG
NgoMIV 1556 GCCGGC G^CCGGC
PflFI 548 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1486 CACGTG CAC^GTG
PmlI 1486 CACGTG CAC^GTG
PpuMI 1523 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 997 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 951 CCTGCAGG CCTGCA^GG
SfoI 1553 GGCGCC GGC^GCC
SmaI 155 CCCGGG CCC^GGG
Sse8387I 951 CCTGCAGG CCTGCA^GG
StuI 251 AGGCCT AGG^CCT
TaqI 344 TCGA T^CGA
TfiI 60 GATTC G^A[AT]TC
Tsp509I 1618 AATT ^AATT
Tth111I 548 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 344 TCGA T^CGA
XhoII 417 AGATCT [GA]^GATC[CT]
XmaCI 155 CCCGGG C^CCGGG
XmaI 155 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 1333 GACGCC G[GA]^CG[CT]C
1553 GGCGCC
AocI 334 CCTGAGG CC^T[ACGT]AGG
1548 CCTGAGG
ApaI 151 GGGCCC GGGCC^C
158 GGGCCC
BlpI 963 GCTTAGC GC^T[ACGT]AGC
1560 GCTGAGC
Bpu1102I 963 GCTTAGC GC^T[ACGT]AGC
1560 GCTGAGC
BsaHI 1333 GACGCC G[GA]^CG[CT]C
1553 GGCGCC
BsaI 37 GAGACC GGTCTC(1/5)
346 GAGACC
BsaWI 266 ACCGGA [AT]^CCGG[AT]
371 TCCGGA
BseRI 1342 GAGGAG GAGGAG(10/8)
1402 GAGGAG
BstEII 888 GGTCACC G^GT[ACGT]ACC
1473 GGTGACC
BstPI 888 GGTCACC G^GT[ACGT]ACC
1473 GGTGACC
Bsu36I 334 CCTGAGG CC^T[ACGT]AGG
1548 CCTGAGG
CelII 963 GCTTAGC GC^T[ACGT]AGC
1560 GCTGAGC
DpnII 177 GATC ^GATC
418 GATC
DsaI 41 CCGTGG C^C[GA][CT]GG
1337 CCATGG
EaeI 92 TGGCCA [CT]^GGCC[GA]
99 CGGCCA
Eco81I 334 CCTGAGG CC^T[ACGT]AGG
1548 CCTGAGG
EcoO65I 888 GGTCACC G^GT[ACGT]ACC
1473 GGTGACC
EcoT14I 1337 CCATGG C^C[AT][AT]GG
1580 CCAAGG
FauI 1 GCGGG CCCGC(4/6)
436 GCGGG
Hin1I 1333 GACGCC G[GA]^CG[CT]C
1553 GGCGCC
MboI 177 GATC ^GATC
418 GATC
MseI 533 TTAA T^TAA
1620 TTAA
NdeII 177 GATC ^GATC
418 GATC
PflMI 909 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1374 CCAGACCCTGG
PstI 406 CTGCAG CTGCA^G
952 CTGCAG
SacI 901 GAGCTC GAGCT^C
1563 GAGCTC
Sau3AI 177 GATC ^GATC
418 GATC
SfcI 406 CTGCAG C^T[GA][CT]AG
952 CTGCAG
SmlI 543 CTCAAG C^T[CT][GA]AG
1014 CTTGAG
StyI 1337 CCATGG C^C[AT][AT]GG
1580 CCAAGG
Tru9I 533 TTAA T^TAA
1620 TTAA
Van91I 909 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1374 CCAGACCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaI AvaII BanII BbvI BcnI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr10I Cfr13I
DdeI DraII EarI EcoNI EcoO109I
EcoRII Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboII MnlI MspA1I MspI MvaI
MwoI NciI NlaIII NlaIV PleI
RsaI Sau96I ScrFI SfaNI TseI
Tsp45I TspRI