##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
AocI Aor51HI AscI AseI AsnI
AspEI AviII AvrII BamHI BbeI
BciVI BclI BfaI BfrI BglII
BlnI BsaBI BsaWI BseAI BsiWI
BsmBI BsmI BspDI BspEI BspLU11I
BsrDI BssHII Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DrdI Eam1105I EarI
Eco47III Eco81I EcoNI EcoO65I EcoRV
EcoT22I FbaI FseI FspI HaeII
HindIII HpaI KasI Ksp632I KspI
MaeI MamI MfeI MluI MroI
MunI NarI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 781 GACGTC GACGT^C
Acc65I 350 GGTACC G^GTACC
AccI 1153 GTCTAC GT^[AC][GT]AC
AccII 759 CGCG CG^CG
AflIII 246 ACACGT A^C[GA][CT]GT
Alw44I 558 GTGCAC G^TGCAC
AlwNI 1207 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 558 GTGCAC G^TGCAC
Asp700I 120 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 350 GGTACC G^GTACC
AspI 1147 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1135 CTCGGG C^[CT]CG[GA]G
BbrPI 247 CACGTG CAC^GTG
BbvCI 961 CCTCAGC CCTCAGC(-5/-2)
BsaAI 247 CACGTG [CT]AC^GT[GA]
BsgI 267 CTGCAC GTGCAG(16/14)
BsoBI 1135 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1319 TGTACA T^GTACA
BspHI 665 TCATGA T^CATGA
BsrFI 76 GCCGGC [GA]^CCGG[CT]
BsrGI 1319 TGTACA T^GTACA
BssSI 1327 CTCGTG CACGAG(-5/-1)
BstAPI 16 GCACTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 759 CGCG CG^CG
BstYI 1459 AGATCC [GA]^GATC[CT]
Cfr10I 76 GCCGGC [GA]^CCGG[CT]
DraIII 1125 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 78 CGGCCG C^GGCCG
EclXI 78 CGGCCG C^GGCCG
Eco52I 78 CGGCCG C^GGCCG
EcoRI 1348 GAATTC G^AATTC
KpnI 350 GGTACC GGTAC^C
MflI 1459 AGATCC [GA]^GATC[CT]
MseI 1172 TTAA T^TAA
MslI 270 CACGATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 759 CGCG CG^CG
NaeI 76 GCCGGC GCC^GGC
NcoI 346 CCATGG C^CATGG
NgoMIV 76 GCCGGC G^CCGGC
PflFI 1147 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 247 CACGTG CAC^GTG
PmlI 247 CACGTG CAC^GTG
PstI 23 CTGCAG CTGCA^G
RcaI 665 TCATGA T^CATGA
SexAI 881 ACCTGGT A^CC[AT]GGT
SfcI 23 CTGCAG C^T[GA][CT]AG
SspBI 1319 TGTACA T^GTACA
StuI 122 AGGCCT AGG^CCT
TfiI 470 GAATC G^A[AT]TC
Tru9I 1172 TTAA T^TAA
Tth111I 1147 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 563 CCAGCTTCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1459 AGATCC [GA]^GATC[CT]
XmnI 120 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 1299 AAATTT [GA]^AATT[CT]
1348 GAATTC
ApoI 1299 AAATTT [GA]^AATT[CT]
1348 GAATTC
BbsI 784 GTCTTC GAAGAC(2/6)
994 GTCTTC
BcgI 560 GCACCAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1260 GCAGATGTCTCG
BpuAI 784 GTCTTC GAAGAC(2/6)
994 GTCTTC
BsaI 141 GAGACC GGTCTC(1/5)
85 GGTCTC
BsmFI 750 GGGAC GGGAC(10/14)
1518 GGGAC
BspMI 353 ACCTGC ACCTGC(4/8)
617 ACCTGC
BsrBI 291 CCGCTC CCGCTC(-3/-3)
57 GAGCGG
DraII 109 GGGGCCC [GA]G^G[ACGT]CC[CT]
1280 GGGCCCT
EcoO109I 109 GGGGCCC [GA]G^G[ACGT]CC[CT]
1280 GGGCCCT
EcoT14I 346 CCATGG C^C[AT][AT]GG
429 CCTTGG
HgaI 1370 GACGC GACGC(5/10)
448 GCGTC
PflMI 372 CCACTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1124 CCACTCTGTGG
SacI 31 GAGCTC GAGCT^C
1195 GAGCTC
SmlI 622 CTCAAG C^T[CT][GA]AG
826 CTCAAG
StyI 346 CCATGG C^C[AT][AT]GG
429 CCTTGG
Van91I 372 CCACTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1124 CCACTCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
ApaI AspHI AvaII BalI BanI
BanII BbvI BcnI BglI BlpI
BmyI BpmI Bpu1102I BsaHI BsaJI
BseRI BsiEI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CelII CfoI Cfr13I
DdeI DpnII DsaI EaeI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I