##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AhdI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AviII AvrII BamHI BbvCI
BcgI BciVI BfrI BglII BlnI
BsaBI BsaI BsiWI BsmI BsoBI
BspDI BspMI BsrDI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI DrdI DsaI Eam1105I Eco47III
Eco81I EcoO65I EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MamI MflI MluI
MseI MvnI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI ScaI SexAI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspI SwaI Tru9I Van91I XbaI
XhoI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AcsI 1403 GAATTC [GA]^AATT[CT]
AgeI 1386 ACCGGT A^CCGGT
AlwNI 146 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 1403 GAATTC [GA]^AATT[CT]
AspI 598 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1689 GGCGCC GGCGC^C
BbrPI 699 CACGTG CAC^GTG
BbsI 1315 GAAGAC GAAGAC(2/6)
BclI 1734 TGATCA T^GATCA
BlpI 626 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 626 GCTGAGC GC^T[ACGT]AGC
BpuAI 1315 GAAGAC GAAGAC(2/6)
BsaAI 699 CACGTG [CT]AC^GT[GA]
BsiEI 168 CGGCCG CG[GA][CT]^CG
BsmBI 911 GAGACG CGTCTC(1/5)
BspHI 1696 TCATGA T^CATGA
BspLU11I 1510 ACATGT A^CATGT
BssSI 1322 CACGAG CACGAG(-5/-1)
BstXI 93 CCAGGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 626 GCTGAGC GC^T[ACGT]AGC
EagI 168 CGGCCG C^GGCCG
EclXI 168 CGGCCG C^GGCCG
Eco52I 168 CGGCCG C^GGCCG
EcoNI 223 CCTCCCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1403 GAATTC G^AATTC
EcoT14I 19 CCTTGG C^C[AT][AT]GG
FbaI 1734 TGATCA T^GATCA
HaeII 1689 GGCGCC [GA]GCGC^[CT]
KasI 1689 GGCGCC G^GCGCC
MfeI 1450 CAATTG C^AATTG
MunI 1450 CAATTG C^AATTG
NarI 1689 GGCGCC GG^CGCC
NspI 1510 ACATGT [GA]CATG^[CT]
PflFI 598 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1386 ACCGGT A^CCGGT
PmaCI 699 CACGTG CAC^GTG
PmlI 699 CACGTG CAC^GTG
PvuII 74 CAGCTG CAG^CTG
RcaI 1696 TCATGA T^CATGA
SapI 1456 GAAGAGC GCTCTTC(1/4)
SbfI 253 CCTGCAGG CCTGCA^GG
SfiI 211 GGCCAGAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1689 GGCGCC GGC^GCC
Sse8387I 253 CCTGCAGG CCTGCA^GG
StyI 19 CCTTGG C^C[AT][AT]GG
TfiI 393 GAATC G^A[AT]TC
Tth111I 598 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 294 GTCTAC GT^[AC][GT]AC
1079 GTCTAC
AccIII 484 TCCGGA T^CCGGA
1374 TCCGGA
AcyI 32 GGCGTC G[GA]^CG[CT]C
1689 GGCGCC
AfaI 781 GTAC GT^AC
1255 GTAC
Alw44I 1108 GTGCAC G^TGCAC
1553 GTGCAC
AlwI 320 GATCC GGATC(4/5)
1372 GATCC
ApaLI 1108 GTGCAC G^TGCAC
1553 GTGCAC
BglI 212 GCCAGAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
233 GCCCTCCGGGC
BsaHI 32 GGCGTC G[GA]^CG[CT]C
1689 GGCGCC
BseAI 484 TCCGGA T^CCGGA
1374 TCCGGA
BsgI 663 GTGCAG GTGCAG(16/14)
1033 GTGCAG
BsmAI 911 GAGAC GTCTC(1/5)
1049 GAGAC
Bsp1407I 780 TGTACA T^GTACA
1254 TGTACA
BspEI 484 TCCGGA T^CCGGA
1374 TCCGGA
BsrBI 629 GAGCGG CCGCTC(-3/-3)
1634 GAGCGG
BsrGI 780 TGTACA T^GTACA
1254 TGTACA
DraIII 282 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
1102 CACCCGGTG
EarI 158 CTCTTC CTCTTC(1/4)
1456 GAAGAG
HgaI 1318 GACGC GACGC(5/10)
33 GCGTC
Hin1I 32 GGCGTC G[GA]^CG[CT]C
1689 GGCGCC
Ksp632I 158 CTCTTC CTCTTC(1/4)
1456 GAAGAG
MroI 484 TCCGGA T^CCGGA
1374 TCCGGA
RsaI 781 GTAC GT^AC
1255 GTAC
SmlI 586 CTTGAG C^T[CT][GA]AG
1526 CTCAAG
SspBI 780 TGTACA T^GTACA
1254 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AflIII AluI AspHI AvaII
BalI BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV PleI PstI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI