##################################
# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII BamHI BbeI
BbrPI BbsI BcgI BciVI BclI
BfrI BlpI Bpu1102I BpuAI BsaAI
BsaHI BsaI BseAI BseRI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII Hin1I HindIII HinfI HpaI
KasI KpnI Ksp632I KspI MaeII
MfeI MluI MroI MseI MslI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI TfiI
Tru9I Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 1 GTCGAC GT^[AC][GT]AC
AcsI 689 AAATTT [GA]^AATT[CT]
AlwNI 41 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 689 AAATTT [GA]^AATT[CT]
AspHI 792 GAGCTC G[AT]GC[AT]^C
AvaI 391 CCCGGG C^[CT]CG[GA]G
BalI 356 TGGCCA TGG^CCA
BanI 312 GGTGCC G^G[CT][GA]CC
BanII 792 GAGCTC G[GA]GC[CT]^C
BbvCI 50 GCTGAGG CCTCAGC(-5/-2)
BglI 254 GCCCGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 804 CTGGAG CTGGAG(16/14)
BsaBI 663 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 79 ACCGGA [AT]^CCGG[AT]
BsiHKAI 792 GAGCTC G[AT]GC[AT]^C
BsmAI 152 GAGAC GTCTC(1/5)
BsmBI 152 GAGACG CGTCTC(1/5)
BsoBI 391 CCCGGG C^[CT]CG[GA]G
BspMI 521 ACCTGC ACCTGC(4/8)
BssHII 95 GCGCGC G^CGCGC
BstXI 243 CCAAGACGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoNI 207 CCTAGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
MamI 663 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 356 TGGCCA TGG^CCA
MscI 356 TGGCCA TGG^CCA
MspA1I 11 CAGCTG C[AC]G^C[GT]G
PflMI 40 CCAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 11 CAGCTG CAG^CTG
SacI 792 GAGCTC GAGCT^C
SalI 1 GTCGAC G^TCGAC
SfcI 852 CTATAG C^T[GA][CT]AG
SmaI 391 CCCGGG CCC^GGG
SspI 471 AATATT AAT^ATT
Tsp509I 690 AATT ^AATT
Van91I 40 CCAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 629 CCAGCAAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 391 CCCGGG C^CCGGG
XmaI 391 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 64 CGCG CG^CG
96 CGCG
AfaI 622 GTAC GT^AC
749 GTAC
AvrII 307 CCTAGG C^CTAGG
372 CCTAGG
BglII 662 AGATCT A^GATCT
767 AGATCT
BlnI 307 CCTAGG C^CTAGG
372 CCTAGG
BmyI 792 GAGCTC G[AGT]GC[ACT]^C
885 GTGCCC
Bsp1286I 792 GAGCTC G[AGT]GC[ACT]^C
885 GTGCCC
BsrI 46 ACTGG ACTGG(1/-1)
870 ACTGG
BstUI 64 CGCG CG^CG
96 CGCG
BstYI 662 AGATCT [GA]^GATC[CT]
767 AGATCT
CfoI 95 GCGC GCG^C
97 GCGC
DpnII 663 GATC ^GATC
768 GATC
DsaI 549 CCACGG C^C[GA][CT]GG
590 CCACGG
HgaI 569 GCGTC GACGC(5/10)
836 GCGTC
HhaI 95 GCGC GCG^C
97 GCGC
HinP1I 95 GCGC G^CGC
97 GCGC
HincII 1 GTCGAC GT[CT]^[GA]AC
932 GTTGAC
HindII 1 GTCGAC GT[CT]^[GA]AC
932 GTTGAC
MboI 663 GATC ^GATC
768 GATC
MboII 174 GAAGA GAAGA(8/7)
665 TCTTC
MflI 662 AGATCT [GA]^GATC[CT]
767 AGATCT
MvnI 64 CGCG CG^CG
96 CGCG
NdeII 663 GATC ^GATC
768 GATC
NlaIII 86 CATG CATG^
289 CATG
RsaI 622 GTAC GT^AC
749 GTAC
Sau3AI 663 GATC ^GATC
768 GATC
SfaNI 177 GATGC GCATC(5/9)
682 GCATC
Tsp45I 529 GTCAC ^GT[GC]AC
779 GTGAC
TspRI 509 CAGTG CA[GC]TG[ACGT][ACGT]^
906 CAGTG
XhoII 662 AGATCT [GA]^GATC[CT]
767 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI AvaII BbvI BcnI
BfaI BsaJI BsgI BsiYI BslI
BsmFI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DraII EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HpaII HphI ItaI
MaeI MaeIII MnlI MspI MvaI
MwoI NciI NlaIV PpuMI Sau96I
ScrFI StyI TaqI TseI TthHB8I