##################################
# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BamHI BanII
BbeI BbrPI BbvCI BcgI BciVI
BcnI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV FauI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI HpaII KasI KpnI KspI
MaeIII MamI MluI MroI MspI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI SspI StuI SwaI Tsp45I
Tth111I XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AciI 5 GCGG CCGC(-3/-1)
AfaI 703 GTAC GT^AC
AflII 1094 CTTAAG C^TTAAG
AlwI 472 GGATC GGATC(4/5)
AlwNI 638 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 620 ATTAAT AT^TAAT
AsnI 620 ATTAAT AT^TAAT
Asp700I 1104 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 961 GGTCC G^G[AT]CC
BalI 1003 TGGCCA TGG^CCA
BanI 754 GGTGCC G^G[CT][GA]CC
BclI 517 TGATCA T^GATCA
BfaI 581 CTAG C^TAG
BfrI 1094 CTTAAG C^TTAAG
BglII 1087 AGATCT A^GATCT
BpmI 898 CTCCAG CTGGAG(16/14)
BsaI 587 GGTCTC GGTCTC(1/5)
BsgI 906 GTGCAG GTGCAG(16/14)
BsmFI 962 GTCCC GGGAC(10/14)
BsmI 163 GCATTC GAATGC(1/-1)
BssKI 723 CCTGG ^CC[ACGT]GG
BstNI 723 CCTGG CC^[AT]GG
BstYI 1087 AGATCT [GA]^GATC[CT]
Cac8I 608 GCTTGC GC[ACGT]^[ACGT]GC
DraI 826 TTTAAA TTT^AAA
DsaI 300 CCATGG C^C[GA][CT]GG
EaeI 1003 TGGCCA [CT]^GGCC[GA]
EcoRII 723 CCTGG ^CC[AT]GG
EcoT22I 857 ATGCAT ATGCA^T
FbaI 517 TGATCA T^GATCA
HindIII 689 AAGCTT A^AGCTT
MaeI 581 CTAG C^TAG
MaeII 97 ACGT A^CGT
MfeI 476 CAATTG C^AATTG
MflI 1087 AGATCT [GA]^GATC[CT]
MluNI 1003 TGGCCA TGG^CCA
MscI 1003 TGGCCA TGG^CCA
MslI 860 CATATAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 476 CAATTG C^AATTG
MvaI 723 CCTGG CC^[AT]GG
NcoI 300 CCATGG C^CATGG
NsiI 857 ATGCAT ATGCA^T
PflMI 578 CCACTAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 112 GACAAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 620 ATTAAT AT^TAAT
RsaI 703 GTAC GT^AC
SapI 53 GAAGAGC GCTCTTC(1/4)
ScrFI 723 CCTGG CC^[ACGT]GG
SpeI 580 ACTAGT A^CTAGT
TaqI 819 TCGA T^CGA
TspRI 780 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 819 TCGA T^CGA
Van91I 578 CCACTAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1087 AGATCT [GA]^GATC[CT]
XmnI 1104 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BbsI 997 GAAGAC GAAGAC(2/6)
119 GTCTTC
BbvI 908 GCAGC GCAGC(8/12)
1019 GCTGC
BmyI 755 GTGCCC G[AGT]GC[ACT]^C
1037 GTGCCC
BpuAI 997 GAAGAC GAAGAC(2/6)
119 GTCTTC
BsiYI 578 CCACTAGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
592 CCCTTTTTTGG
BslI 578 CCACTAGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
592 CCCTTTTTTGG
Bsp1286I 755 GTGCCC G[AGT]GC[ACT]^C
1037 GTGCCC
BstAPI 748 GCATCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
908 GCAGCATTTGC
BstF5I 7 GGATG GGATG(2/0)
496 GGATG
Fnu4HI 908 GCAGC GC^[ACGT]GC
1019 GCTGC
FokI 7 GGATG GGATG(9/13)
496 GGATG
ItaI 908 GCAGC GC^[ACGT]GC
1019 GCTGC
MspA1I 168 CAGCTG C[AC]G^C[GT]G
350 CAGCTG
NlaIII 301 CATG CATG^
1007 CATG
PvuII 168 CAGCTG CAG^CTG
350 CAGCTG
SmlI 224 CTCAAG C^T[CT][GA]AG
1094 CTTAAG
TseI 908 GCAGC G^C[AT]GC
1019 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AcsI AluI ApoI BsaJI BseRI
BsmAI BsrI Cfr13I DdeI DpnII
EarI EcoT14I HaeIII HinfI HphI
Ksp632I MboI MboII MnlI MseI
MwoI NdeII NlaIV Sau3AI Sau96I
SfaNI StyI TfiI Tru9I Tsp509I