##################################
# NO CUT ENZYMES ( 158 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BciVI BclI BcnI BfrI
BglI BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoT14I EcoT22I FbaI FseI
FspI HaeII HapII HhaI Hin1I
HinP1I HindIII HpaI HpaII KasI
KpnI KspI MamI MfeI MluI
MroI MslI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 538 GTAGAC GT^[AC][GT]AC
AciI 181 GCGG CCGC(-3/-1)
AfaI 592 GTAC GT^AC
AlwI 386 GATCC GGATC(4/5)
Asp700I 410 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 856 GGACC G^G[AT]CC
BalI 218 TGGCCA TGG^CCA
BanII 13 GAGCCC G[GA]GC[CT]^C
BbsI 468 GAAGAC GAAGAC(2/6)
BcgI 104 CGAAGAGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 440 AGATCT A^GATCT
BmyI 13 GAGCCC G[AGT]GC[ACT]^C
BpuAI 468 GAAGAC GAAGAC(2/6)
BsaI 267 GAGACC GGTCTC(1/5)
BseRI 580 GAGGAG GAGGAG(10/8)
BsgI 463 GTGCAG GTGCAG(16/14)
BsmI 647 GCATTC GAATGC(1/-1)
Bsp1286I 13 GAGCCC G[AGT]GC[ACT]^C
BspHI 154 TCATGA T^CATGA
BspMI 311 ACCTGC ACCTGC(4/8)
BsrBI 179 GAGCGG CCGCTC(-3/-3)
BstBI 70 TTCGAA TT^CGAA
BstF5I 492 CATCC GGATG(2/0)
Cac8I 110 GCTTGC GC[ACGT]^[ACGT]GC
DsaI 495 CCGTGG C^C[GA][CT]GG
EaeI 218 TGGCCA [CT]^GGCC[GA]
EcoNI 25 CCTTTCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 905 GAATTC G^AATTC
FauI 181 GCGGG CCCGC(4/6)
FokI 492 CATCC GGATG(9/13)
HgaI 126 GCGTC GACGC(5/10)
HincII 636 GTTGAC GT[CT]^[GA]AC
HindII 636 GTTGAC GT[CT]^[GA]AC
HphI 425 TCACC GGTGA(8/7)
MaeII 50 ACGT A^CGT
MluNI 218 TGGCCA TGG^CCA
MscI 218 TGGCCA TGG^CCA
MwoI 835 GCAGCCCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspV 70 TTCGAA TT^CGAA
PpuMI 855 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 154 TCATGA T^CATGA
RsaI 592 GTAC GT^AC
SapI 105 GAAGAGC GCTCTTC(1/4)
SfcI 76 CTGTAG C^T[GA][CT]AG
SfuI 70 TTCGAA TT^CGAA
SmlI 570 CTTGAG C^T[CT][GA]AG
Tsp45I 658 GTGAC ^GT[GC]AC
XmnI 410 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 762 GAATTT [GA]^AATT[CT]
905 GAATTC
ApoI 762 GAATTT [GA]^AATT[CT]
905 GAATTC
BbvI 835 GCAGC GCAGC(8/12)
869 GCAGC
BfaI 229 CTAG C^TAG
831 CTAG
BsrDI 122 CATTGC GCAATG(2/0)
193 GCAATG
BstYI 385 AGATCC [GA]^GATC[CT]
440 AGATCT
Cfr13I 369 GGGCC G^G[ACGT]CC
856 GGACC
DraII 368 AGGGCCT [GA]G^G[ACGT]CC[CT]
855 GGGACCC
EcoO109I 368 AGGGCCT [GA]G^G[ACGT]CC[CT]
855 GGGACCC
EcoRV 894 GATATC GAT^ATC
929 GATATC
Fnu4HI 835 GCAGC GC^[ACGT]GC
869 GCAGC
ItaI 835 GCAGC GC^[ACGT]GC
869 GCAGC
MaeI 229 CTAG C^TAG
831 CTAG
MaeIII 523 GTAAC ^GT[ACGT]AC
658 GTGAC
MflI 385 AGATCC [GA]^GATC[CT]
440 AGATCT
NlaIII 155 CATG CATG^
473 CATG
PleI 822 GACTC GAGTC(4/5)
984 GAGTC
Sau96I 369 GGGCC G^G[ACGT]CC
856 GGACC
TfiI 35 GAATC G^A[AT]TC
506 GATTC
TseI 835 GCAGC G^C[AT]GC
869 GCAGC
XhoII 385 AGATCC [GA]^GATC[CT]
440 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AluI BsaJI BsiYI BslI BsmAI
BsmFI BsrI BssKI BstNI DdeI
DpnII EarI EcoRII HaeIII HinfI
Ksp632I MboI MboII MnlI MseI
MvaI NdeII NlaIV Sau3AI ScrFI
SfaNI TaqI Tru9I Tsp509I TspRI
TthHB8I