##################################
# NO CUT ENZYMES (  151 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        AocI        Aor51HI     ApaI        AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AviII       AvrII       BamHI       BanI        BbeI        
BbrPI       BbsI        BbvCI       BclI        BcnI        
BfrI        BglII       BlnI        BpuAI       BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BseRI       
BsiEI       BsiWI       BsmBI       Bsp1407I    BspDI       
BspEI       BspLU11I    BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstZ17I     
Bsu36I      Cfr10I      ClaI        CpoI        DraI        
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HapII       HgaI        Hin1I       HincII      
HindII      HpaI        HpaII       KasI        KpnI        
KspI        MamI        MfeI        MluI        MroI        
MseI        MspI        MunI        MvnI        NaeI        
NarI        NciI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tru9I       Tth111I     XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AfaI          675  GTAC                 GT^AC
Alw44I        659  GTGCAC               G^TGCAC
AlwI          384  GATCC                GGATC(4/5)
AlwNI         169  CAGTTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         659  GTGCAC               G^TGCAC
Asp700I        99  GAAATTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         659  GTGCAC               G[AT]GC[AT]^C
AvaI          442  CTCGGG               C^[CT]CG[GA]G
BalI          914  TGGCCA               TGG^CCA
BanII         586  GAGCCC               G[GA]GC[CT]^C
BcgI          975  CGAAGAATCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         156  GGATAC               GTATCC(6/5)
BglI          916  GCCATGGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI          667  GGTCTC               GGTCTC(1/5)
BsgI          718  CTGCAC               GTGCAG(16/14)
BsiHKAI       659  GTGCAC               G[AT]GC[AT]^C
BsoBI         442  CTCGGG               C^[CT]CG[GA]G
BspHI         965  TCATGA               T^CATGA
BsrI          108  CCAGT                ACTGG(1/-1)
BstXI         590  CCAAAGAGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         383  AGATCC               [GA]^GATC[CT]
DraII           8  AGGACCC              [GA]G^G[ACGT]CC[CT]
DraIII         14  CACATTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          914  TGGCCA               [CT]^GGCC[GA]
EcoO109I        8  AGGACCC              [GA]G^G[ACGT]CC[CT]
HaeII         938  GGCGCT               [GA]GCGC^[CT]
MaeII         875  ACGT                 A^CGT
MaeIII        877  GTCAC                ^GT[ACGT]AC
MflI          383  AGATCC               [GA]^GATC[CT]
MluNI         914  TGGCCA               TGG^CCA
MscI          914  TGGCCA               TGG^CCA
MwoI          916  GCCATGGCGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI          917  CCATGG               C^CATGG
PflMI          13  CCACATTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI           8  AGGACCC              [GA]G^G[AT]CC[CT]
RcaI          965  TCATGA               T^CATGA
RsaI          675  GTAC                 GT^AC
TaqI          389  TCGA                 T^CGA
Tsp45I        877  GTCAC                ^GT[GC]AC
TthHB8I       389  TCGA                 T^CGA
Van91I         13  CCACATTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          654  CCAGGGTGCACTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         383  AGATCC               [GA]^GATC[CT]
XmnI           99  GAAATTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AvaII           9  GGACC                G^G[AT]CC
              341  GGACC               
BlpI           36  GCTGAGC              GC^T[ACGT]AGC
              739  GCTGAGC             
BmyI          586  GAGCCC               G[AGT]GC[ACT]^C
              659  GTGCAC              
BpmI          946  CTCCAG               CTGGAG(16/14)
              646  CTGGAG              
Bpu1102I       36  GCTGAGC              GC^T[ACGT]AGC
              739  GCTGAGC             
BsmI          618  GCATTC               GAATGC(1/-1)
             1080  GCATTC              
Bsp1286I      586  GAGCCC               G[AGT]GC[ACT]^C
              659  GTGCAC              
BsrDI         164  CATTGC               GCAATG(2/0)
              345  CATTGC              
Cac8I         510  GCTTGC               GC[ACGT]^[ACGT]GC
             1006  GCTTGC              
CelII          36  GCTGAGC              GC^T[ACGT]AGC
              739  GCTGAGC             
CfoI          550  GCGC                 GCG^C
              939  GCGC                
Cfr13I          9  GGACC                G^G[ACGT]CC
              341  GGACC               
DpnII         384  GATC                 ^GATC
              479  GATC                
DsaI          917  CCATGG               C^C[GA][CT]GG
              933  CCACGG              
EcoT14I       260  CCTTGG               C^C[AT][AT]GG
              917  CCATGG              
HaeIII        223  GGCC                 GG^CC
              915  GGCC                
HhaI          550  GCGC                 GCG^C
              939  GCGC                
HinP1I        550  GCGC                 G^CGC
              939  GCGC                
HindIII       626  AAGCTT               A^AGCTT
             1004  AAGCTT              
HphI          471  GGTGA                GGTGA(8/7)
             1056  TCACC               
MboI          384  GATC                 ^GATC
              479  GATC                
MslI          858  CATCCCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              912  CATGGCCATG          
MspA1I        737  CAGCTG               C[AC]G^C[GT]G
              949  CAGCTG              
NdeII         384  GATC                 ^GATC
              479  GATC                
NlaIV           9  GGACCC               GG[ACGT]^[ACGT]CC
              340  GGGACC              
PvuII         737  CAGCTG               CAG^CTG
              949  CAGCTG              
Sau3AI        384  GATC                 ^GATC
              479  GATC                
Sau96I          9  GGACC                G^G[ACGT]CC
              341  GGACC               
StyI          260  CCTTGG               C^C[AT][AT]GG
              917  CCATGG              
TfiI          692  GAATC                G^A[AT]TC
              979  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   33 enzymes) #
#########################################

AciI        AcsI        AluI        ApoI        BbvI        
BfaI        BsaJI       BsiYI       BslI        BsmAI       
BsmFI       BssKI       BstF5I      BstNI       DdeI        
EarI        EcoRII      Fnu4HI      FokI        HinfI       
ItaI        Ksp632I     MaeI        MboII       MnlI        
MvaI        NlaIII      PleI        ScrFI       SfaNI       
TseI        Tsp509I     TspRI