##################################
# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AocI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BclI BcnI
BfrI BglII BlnI BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HapII HgaI Hin1I HincII
HindII HpaI HpaII KasI KpnI
KspI MamI MfeI MluI MroI
MseI MspI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tth111I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AfaI 675 GTAC GT^AC
Alw44I 659 GTGCAC G^TGCAC
AlwI 384 GATCC GGATC(4/5)
AlwNI 169 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 659 GTGCAC G^TGCAC
Asp700I 99 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 659 GTGCAC G[AT]GC[AT]^C
AvaI 442 CTCGGG C^[CT]CG[GA]G
BalI 914 TGGCCA TGG^CCA
BanII 586 GAGCCC G[GA]GC[CT]^C
BcgI 975 CGAAGAATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 156 GGATAC GTATCC(6/5)
BglI 916 GCCATGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 667 GGTCTC GGTCTC(1/5)
BsgI 718 CTGCAC GTGCAG(16/14)
BsiHKAI 659 GTGCAC G[AT]GC[AT]^C
BsoBI 442 CTCGGG C^[CT]CG[GA]G
BspHI 965 TCATGA T^CATGA
BsrI 108 CCAGT ACTGG(1/-1)
BstXI 590 CCAAAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 383 AGATCC [GA]^GATC[CT]
DraII 8 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 14 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 914 TGGCCA [CT]^GGCC[GA]
EcoO109I 8 AGGACCC [GA]G^G[ACGT]CC[CT]
HaeII 938 GGCGCT [GA]GCGC^[CT]
MaeII 875 ACGT A^CGT
MaeIII 877 GTCAC ^GT[ACGT]AC
MflI 383 AGATCC [GA]^GATC[CT]
MluNI 914 TGGCCA TGG^CCA
MscI 914 TGGCCA TGG^CCA
MwoI 916 GCCATGGCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 917 CCATGG C^CATGG
PflMI 13 CCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 8 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 965 TCATGA T^CATGA
RsaI 675 GTAC GT^AC
TaqI 389 TCGA T^CGA
Tsp45I 877 GTCAC ^GT[GC]AC
TthHB8I 389 TCGA T^CGA
Van91I 13 CCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 654 CCAGGGTGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 383 AGATCC [GA]^GATC[CT]
XmnI 99 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AvaII 9 GGACC G^G[AT]CC
341 GGACC
BlpI 36 GCTGAGC GC^T[ACGT]AGC
739 GCTGAGC
BmyI 586 GAGCCC G[AGT]GC[ACT]^C
659 GTGCAC
BpmI 946 CTCCAG CTGGAG(16/14)
646 CTGGAG
Bpu1102I 36 GCTGAGC GC^T[ACGT]AGC
739 GCTGAGC
BsmI 618 GCATTC GAATGC(1/-1)
1080 GCATTC
Bsp1286I 586 GAGCCC G[AGT]GC[ACT]^C
659 GTGCAC
BsrDI 164 CATTGC GCAATG(2/0)
345 CATTGC
Cac8I 510 GCTTGC GC[ACGT]^[ACGT]GC
1006 GCTTGC
CelII 36 GCTGAGC GC^T[ACGT]AGC
739 GCTGAGC
CfoI 550 GCGC GCG^C
939 GCGC
Cfr13I 9 GGACC G^G[ACGT]CC
341 GGACC
DpnII 384 GATC ^GATC
479 GATC
DsaI 917 CCATGG C^C[GA][CT]GG
933 CCACGG
EcoT14I 260 CCTTGG C^C[AT][AT]GG
917 CCATGG
HaeIII 223 GGCC GG^CC
915 GGCC
HhaI 550 GCGC GCG^C
939 GCGC
HinP1I 550 GCGC G^CGC
939 GCGC
HindIII 626 AAGCTT A^AGCTT
1004 AAGCTT
HphI 471 GGTGA GGTGA(8/7)
1056 TCACC
MboI 384 GATC ^GATC
479 GATC
MslI 858 CATCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
912 CATGGCCATG
MspA1I 737 CAGCTG C[AC]G^C[GT]G
949 CAGCTG
NdeII 384 GATC ^GATC
479 GATC
NlaIV 9 GGACCC GG[ACGT]^[ACGT]CC
340 GGGACC
PvuII 737 CAGCTG CAG^CTG
949 CAGCTG
Sau3AI 384 GATC ^GATC
479 GATC
Sau96I 9 GGACC G^G[ACGT]CC
341 GGACC
StyI 260 CCTTGG C^C[AT][AT]GG
917 CCATGG
TfiI 692 GAATC G^A[AT]TC
979 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AcsI AluI ApoI BbvI
BfaI BsaJI BsiYI BslI BsmAI
BsmFI BssKI BstF5I BstNI DdeI
EarI EcoRII Fnu4HI FokI HinfI
ItaI Ksp632I MaeI MboII MnlI
MvaI NlaIII PleI ScrFI SfaNI
TseI Tsp509I TspRI