################################## # NO CUT ENZYMES ( 151 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BclI BcnI BfrI BglII BlnI BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HapII HgaI Hin1I HincII HindII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MroI MseI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AfaI 675 GTAC GT^AC Alw44I 659 GTGCAC G^TGCAC AlwI 384 GATCC GGATC(4/5) AlwNI 169 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 659 GTGCAC G^TGCAC Asp700I 99 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 659 GTGCAC G[AT]GC[AT]^C AvaI 442 CTCGGG C^[CT]CG[GA]G BalI 914 TGGCCA TGG^CCA BanII 586 GAGCCC G[GA]GC[CT]^C BcgI 975 CGAAGAATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 156 GGATAC GTATCC(6/5) BglI 916 GCCATGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaI 667 GGTCTC GGTCTC(1/5) BsgI 718 CTGCAC GTGCAG(16/14) BsiHKAI 659 GTGCAC G[AT]GC[AT]^C BsoBI 442 CTCGGG C^[CT]CG[GA]G BspHI 965 TCATGA T^CATGA BsrI 108 CCAGT ACTGG(1/-1) BstXI 590 CCAAAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 383 AGATCC [GA]^GATC[CT] DraII 8 AGGACCC [GA]G^G[ACGT]CC[CT] DraIII 14 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 914 TGGCCA [CT]^GGCC[GA] EcoO109I 8 AGGACCC [GA]G^G[ACGT]CC[CT] HaeII 938 GGCGCT [GA]GCGC^[CT] MaeII 875 ACGT A^CGT MaeIII 877 GTCAC ^GT[ACGT]AC MflI 383 AGATCC [GA]^GATC[CT] MluNI 914 TGGCCA TGG^CCA MscI 914 TGGCCA TGG^CCA MwoI 916 GCCATGGCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 917 CCATGG C^CATGG PflMI 13 CCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 8 AGGACCC [GA]G^G[AT]CC[CT] RcaI 965 TCATGA T^CATGA RsaI 675 GTAC GT^AC TaqI 389 TCGA T^CGA Tsp45I 877 GTCAC ^GT[GC]AC TthHB8I 389 TCGA T^CGA Van91I 13 CCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 654 CCAGGGTGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 383 AGATCC [GA]^GATC[CT] XmnI 99 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AvaII 9 GGACC G^G[AT]CC 341 GGACC BlpI 36 GCTGAGC GC^T[ACGT]AGC 739 GCTGAGC BmyI 586 GAGCCC G[AGT]GC[ACT]^C 659 GTGCAC BpmI 946 CTCCAG CTGGAG(16/14) 646 CTGGAG Bpu1102I 36 GCTGAGC GC^T[ACGT]AGC 739 GCTGAGC BsmI 618 GCATTC GAATGC(1/-1) 1080 GCATTC Bsp1286I 586 GAGCCC G[AGT]GC[ACT]^C 659 GTGCAC BsrDI 164 CATTGC GCAATG(2/0) 345 CATTGC Cac8I 510 GCTTGC GC[ACGT]^[ACGT]GC 1006 GCTTGC CelII 36 GCTGAGC GC^T[ACGT]AGC 739 GCTGAGC CfoI 550 GCGC GCG^C 939 GCGC Cfr13I 9 GGACC G^G[ACGT]CC 341 GGACC DpnII 384 GATC ^GATC 479 GATC DsaI 917 CCATGG C^C[GA][CT]GG 933 CCACGG EcoT14I 260 CCTTGG C^C[AT][AT]GG 917 CCATGG HaeIII 223 GGCC GG^CC 915 GGCC HhaI 550 GCGC GCG^C 939 GCGC HinP1I 550 GCGC G^CGC 939 GCGC HindIII 626 AAGCTT A^AGCTT 1004 AAGCTT HphI 471 GGTGA GGTGA(8/7) 1056 TCACC MboI 384 GATC ^GATC 479 GATC MslI 858 CATCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 912 CATGGCCATG MspA1I 737 CAGCTG C[AC]G^C[GT]G 949 CAGCTG NdeII 384 GATC ^GATC 479 GATC NlaIV 9 GGACCC GG[ACGT]^[ACGT]CC 340 GGGACC PvuII 737 CAGCTG CAG^CTG 949 CAGCTG Sau3AI 384 GATC ^GATC 479 GATC Sau96I 9 GGACC G^G[ACGT]CC 341 GGACC StyI 260 CCTTGG C^C[AT][AT]GG 917 CCATGG TfiI 692 GAATC G^A[AT]TC 979 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AciI AcsI AluI ApoI BbvI BfaI BsaJI BsiYI BslI BsmAI BsmFI BssKI BstF5I BstNI DdeI EarI EcoRII Fnu4HI FokI HinfI ItaI Ksp632I MaeI MboII MnlI MvaI NlaIII PleI ScrFI SfaNI TseI Tsp509I TspRI