################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BsaI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstBI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NaeI NarI NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AcyI 76 GACGCC G[GA]^CG[CT]C AfaI 177 GTAC GT^AC Alw44I 426 GTGCAC G^TGCAC AlwNI 739 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 426 GTGCAC G^TGCAC AspHI 426 GTGCAC G[AT]GC[AT]^C AspI 76 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 399 TGGCCA TGG^CCA BcnI 42 CCCGG CC^[GC]GG BlpI 51 GCTGAGC GC^T[ACGT]AGC BmyI 426 GTGCAC G[AGT]GC[ACT]^C Bpu1102I 51 GCTGAGC GC^T[ACGT]AGC BsaHI 76 GACGCC G[GA]^CG[CT]C BsaWI 1067 ACCGGA [AT]^CCGG[AT] BsiHKAI 426 GTGCAC G[AT]GC[AT]^C BsmBI 774 CGTCTC CGTCTC(1/5) Bsp1286I 426 GTGCAC G[AGT]GC[ACT]^C BspHI 1089 TCATGA T^CATGA BspMI 752 ACCTGC ACCTGC(4/8) BssSI 762 CACGAG CACGAG(-5/-1) BstAPI 605 GCATCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1146 GGTGACC G^GT[ACGT]ACC BstPI 1146 GGTGACC G^GT[ACGT]ACC BstXI 261 CCAGGATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 14 AGATCC [GA]^GATC[CT] CelII 51 GCTGAGC GC^T[ACGT]AGC DraII 623 GGGACCC [GA]G^G[ACGT]CC[CT] DrdI 661 GACAACAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 623 GGGACCC [GA]G^G[ACGT]CC[CT] EcoO65I 1146 GGTGACC G^GT[ACGT]ACC FauI 437 GCGGG CCCGC(4/6) Hin1I 76 GACGCC G[GA]^CG[CT]C MaeII 290 ACGT A^CGT MflI 14 AGATCC [GA]^GATC[CT] MluNI 399 TGGCCA TGG^CCA MscI 399 TGGCCA TGG^CCA MseI 305 TTAA T^TAA MslI 798 CATTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NciI 42 CCCGG CC^[GC]GG NdeI 1114 CATATG CA^TATG NheI 127 GCTAGC G^CTAGC PflFI 76 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 195 CCACACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 623 GGGACCC [GA]G^G[AT]CC[CT] PshAI 856 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1089 TCATGA T^CATGA RsaI 177 GTAC GT^AC ScaI 176 AGTACT AGT^ACT StuI 25 AGGCCT AGG^CCT TaqI 1026 TCGA T^CGA TfiI 1036 GATTC G^A[AT]TC Tru9I 305 TTAA T^TAA Tth111I 76 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1026 TCGA T^CGA Van91I 195 CCACACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 14 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcsI 283 GAATTT [GA]^AATT[CT] 315 GAATTT AlwI 15 GATCC GGATC(4/5) 1235 GATCC ApoI 283 GAATTT [GA]^AATT[CT] 315 GAATTT AvaI 528 CTCGGG C^[CT]CG[GA]G 648 CTCGGG BclI 119 TGATCA T^GATCA 389 TGATCA BfaI 128 CTAG C^TAG 1078 CTAG BpmI 21 CTCCAG CTGGAG(16/14) 553 CTGGAG BseRI 709 CTCCTC GAGGAG(10/8) 836 CTCCTC BsgI 736 GTGCAG GTGCAG(16/14) 975 GTGCAG BsmAI 164 GAGAC GTCTC(1/5) 775 GTCTC BsoBI 528 CTCGGG C^[CT]CG[GA]G 648 CTCGGG BsrDI 798 CATTGC GCAATG(2/0) 1128 CATTGC CfoI 91 GCGC GCG^C 432 GCGC DsaI 221 CCATGG C^C[GA][CT]GG 1121 CCATGG EarI 643 CTCTTC CTCTTC(1/4) 829 GAAGAG FbaI 119 TGATCA T^GATCA 389 TGATCA HgaI 76 GACGC GACGC(5/10) 668 GCGTC HhaI 91 GCGC GCG^C 432 GCGC HinP1I 91 GCGC G^CGC 432 GCGC Ksp632I 643 CTCTTC CTCTTC(1/4) 829 GAAGAG MaeI 128 CTAG C^TAG 1078 CTAG MspA1I 168 CAGCGG C[AC]G^C[GT]G 435 CAGCGG NcoI 221 CCATGG C^CATGG 1121 CCATGG SmlI 586 CTCAAG C^T[CT][GA]AG 927 CTTGAG Tsp509I 284 AATT ^AATT 316 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AccII AciI AluI AvaII BbvI BsaJI BsiYI BslI BsmFI BsrI BssKI BstF5I BstNI BstUI Cac8I Cfr13I DdeI DpnII EaeI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PleI PstI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI Tsp45I TspRI