##################################
# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BfrI
BglI BglII BlnI BpuAI BsaAI
BsaBI BsaI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII Bst1107I
BstBI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV EcoT22I FseI FspI
HaeII HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI NaeI NarI
NgoMIV NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
XbaI XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AcyI 76 GACGCC G[GA]^CG[CT]C
AfaI 177 GTAC GT^AC
Alw44I 426 GTGCAC G^TGCAC
AlwNI 739 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 426 GTGCAC G^TGCAC
AspHI 426 GTGCAC G[AT]GC[AT]^C
AspI 76 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 399 TGGCCA TGG^CCA
BcnI 42 CCCGG CC^[GC]GG
BlpI 51 GCTGAGC GC^T[ACGT]AGC
BmyI 426 GTGCAC G[AGT]GC[ACT]^C
Bpu1102I 51 GCTGAGC GC^T[ACGT]AGC
BsaHI 76 GACGCC G[GA]^CG[CT]C
BsaWI 1067 ACCGGA [AT]^CCGG[AT]
BsiHKAI 426 GTGCAC G[AT]GC[AT]^C
BsmBI 774 CGTCTC CGTCTC(1/5)
Bsp1286I 426 GTGCAC G[AGT]GC[ACT]^C
BspHI 1089 TCATGA T^CATGA
BspMI 752 ACCTGC ACCTGC(4/8)
BssSI 762 CACGAG CACGAG(-5/-1)
BstAPI 605 GCATCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1146 GGTGACC G^GT[ACGT]ACC
BstPI 1146 GGTGACC G^GT[ACGT]ACC
BstXI 261 CCAGGATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 14 AGATCC [GA]^GATC[CT]
CelII 51 GCTGAGC GC^T[ACGT]AGC
DraII 623 GGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 661 GACAACAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 623 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 1146 GGTGACC G^GT[ACGT]ACC
FauI 437 GCGGG CCCGC(4/6)
Hin1I 76 GACGCC G[GA]^CG[CT]C
MaeII 290 ACGT A^CGT
MflI 14 AGATCC [GA]^GATC[CT]
MluNI 399 TGGCCA TGG^CCA
MscI 399 TGGCCA TGG^CCA
MseI 305 TTAA T^TAA
MslI 798 CATTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 42 CCCGG CC^[GC]GG
NdeI 1114 CATATG CA^TATG
NheI 127 GCTAGC G^CTAGC
PflFI 76 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 195 CCACACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 623 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 856 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1089 TCATGA T^CATGA
RsaI 177 GTAC GT^AC
ScaI 176 AGTACT AGT^ACT
StuI 25 AGGCCT AGG^CCT
TaqI 1026 TCGA T^CGA
TfiI 1036 GATTC G^A[AT]TC
Tru9I 305 TTAA T^TAA
Tth111I 76 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1026 TCGA T^CGA
Van91I 195 CCACACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 14 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 283 GAATTT [GA]^AATT[CT]
315 GAATTT
AlwI 15 GATCC GGATC(4/5)
1235 GATCC
ApoI 283 GAATTT [GA]^AATT[CT]
315 GAATTT
AvaI 528 CTCGGG C^[CT]CG[GA]G
648 CTCGGG
BclI 119 TGATCA T^GATCA
389 TGATCA
BfaI 128 CTAG C^TAG
1078 CTAG
BpmI 21 CTCCAG CTGGAG(16/14)
553 CTGGAG
BseRI 709 CTCCTC GAGGAG(10/8)
836 CTCCTC
BsgI 736 GTGCAG GTGCAG(16/14)
975 GTGCAG
BsmAI 164 GAGAC GTCTC(1/5)
775 GTCTC
BsoBI 528 CTCGGG C^[CT]CG[GA]G
648 CTCGGG
BsrDI 798 CATTGC GCAATG(2/0)
1128 CATTGC
CfoI 91 GCGC GCG^C
432 GCGC
DsaI 221 CCATGG C^C[GA][CT]GG
1121 CCATGG
EarI 643 CTCTTC CTCTTC(1/4)
829 GAAGAG
FbaI 119 TGATCA T^GATCA
389 TGATCA
HgaI 76 GACGC GACGC(5/10)
668 GCGTC
HhaI 91 GCGC GCG^C
432 GCGC
HinP1I 91 GCGC G^CGC
432 GCGC
Ksp632I 643 CTCTTC CTCTTC(1/4)
829 GAAGAG
MaeI 128 CTAG C^TAG
1078 CTAG
MspA1I 168 CAGCGG C[AC]G^C[GT]G
435 CAGCGG
NcoI 221 CCATGG C^CATGG
1121 CCATGG
SmlI 586 CTCAAG C^T[CT][GA]AG
927 CTTGAG
Tsp509I 284 AATT ^AATT
316 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AccII AciI AluI AvaII BbvI
BsaJI BsiYI BslI BsmFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
Cfr13I DdeI DpnII EaeI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
PleI PstI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TseI Tsp45I
TspRI