##################################
# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BfrI
BlnI BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV EcoT14I
EcoT22I FseI HaeII Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MamI MfeI MluI
MroI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccII 1061 CGCG CG^CG
AcsI 813 GAATTC [GA]^AATT[CT]
AflIII 1146 ACATGT A^C[GA][CT]GT
Alw44I 434 GTGCAC G^TGCAC
AlwI 413 GATCC GGATC(4/5)
ApaLI 434 GTGCAC G^TGCAC
ApoI 813 GAATTC [GA]^AATT[CT]
Asp700I 596 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 434 GTGCAC G[AT]GC[AT]^C
AvaI 937 CTCGGG C^[CT]CG[GA]G
AvaII 494 GGTCC G^G[AT]CC
AviII 154 TGCGCA TGC^GCA
BalI 112 TGGCCA TGG^CCA
BcnI 1089 CCCGG CC^[GC]GG
BfaI 372 CTAG C^TAG
BglI 399 GCCGACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 515 AGATCT A^GATCT
BlpI 750 GCTGAGC GC^T[ACGT]AGC
BmyI 434 GTGCAC G[AGT]GC[ACT]^C
Bpu1102I 750 GCTGAGC GC^T[ACGT]AGC
BsgI 147 GTGCAG GTGCAG(16/14)
BsiHKAI 434 GTGCAC G[AT]GC[AT]^C
BsmAI 935 GTCTC GTCTC(1/5)
BsoBI 937 CTCGGG C^[CT]CG[GA]G
Bsp1286I 434 GTGCAC G[AGT]GC[ACT]^C
BspLU11I 1146 ACATGT A^CATGT
BspMI 614 GCAGGT ACCTGC(4/8)
BsrI 880 ACTGG ACTGG(1/-1)
BstUI 1061 CGCG CG^CG
BstYI 515 AGATCT [GA]^GATC[CT]
CelII 750 GCTGAGC GC^T[ACGT]AGC
DraII 847 AGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 112 TGGCCA [CT]^GGCC[GA]
EarI 135 GAAGAG CTCTTC(1/4)
EcoNI 590 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 847 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 813 GAATTC G^AATTC
FspI 154 TGCGCA TGC^GCA
HapII 1090 CCGG C^CGG
HgaI 1001 GCGTC GACGC(5/10)
HpaII 1090 CCGG C^CGG
Ksp632I 135 GAAGAG CTCTTC(1/4)
MaeI 372 CTAG C^TAG
MflI 515 AGATCT [GA]^GATC[CT]
MluNI 112 TGGCCA TGG^CCA
MscI 112 TGGCCA TGG^CCA
MslI 276 CATCAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1090 CCGG C^CGG
MvnI 1061 CGCG CG^CG
NciI 1089 CCCGG CC^[GC]GG
PflMI 293 CCACATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfaNI 396 GATGC GCATC(5/9)
SpeI 371 ACTAGT A^CTAGT
Van91I 293 CCACATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 515 AGATCT [GA]^GATC[CT]
XmnI 596 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 177 GTAC GT^AC
944 GTAC
BclI 121 TGATCA T^GATCA
535 TGATCA
BsaJI 391 CCCTGG C^C[ACGT][ACGT]GG
727 CCCAGG
BseRI 138 GAGGAG GAGGAG(10/8)
579 GAGGAG
BsmFI 389 GTCCC GGGAC(10/14)
620 GTCCC
BsrBI 855 CCGCTC CCGCTC(-3/-3)
213 GAGCGG
BstF5I 281 GGATG GGATG(2/0)
395 GGATG
CfoI 155 GCGC GCG^C
1060 GCGC
FauI 854 CCCGC CCCGC(4/6)
215 GCGGG
FbaI 121 TGATCA T^GATCA
535 TGATCA
FokI 281 GGATG GGATG(9/13)
395 GGATG
HhaI 155 GCGC GCG^C
1060 GCGC
HinP1I 155 GCGC G^CGC
1060 GCGC
HinfI 316 GATTC G^A[ACGT]TC
419 GATTC
HphI 797 GGTGA GGTGA(8/7)
124 TCACC
MseI 505 TTAA T^TAA
1075 TTAA
NspI 1015 ACATGC [GA]CATG^[CT]
1146 ACATGT
PstI 827 CTGCAG CTGCA^G
991 CTGCAG
RsaI 177 GTAC GT^AC
944 GTAC
SfcI 827 CTGCAG C^T[GA][CT]AG
991 CTGCAG
TfiI 316 GATTC G^A[AT]TC
419 GATTC
Tru9I 505 TTAA T^TAA
1075 TTAA
Tsp45I 9 GTGAC ^GT[GC]AC
360 GTGAC
Tsp509I 544 AATT ^AATT
814 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AluI BbvI BpmI BsiYI
BslI BssKI BstNI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI HaeIII
ItaI MaeIII MboI MboII MnlI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PvuII Sau3AI Sau96I ScrFI
TaqI TseI TspRI TthHB8I