################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BfrI BlnI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT14I EcoT22I FseI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccII 1061 CGCG CG^CG AcsI 813 GAATTC [GA]^AATT[CT] AflIII 1146 ACATGT A^C[GA][CT]GT Alw44I 434 GTGCAC G^TGCAC AlwI 413 GATCC GGATC(4/5) ApaLI 434 GTGCAC G^TGCAC ApoI 813 GAATTC [GA]^AATT[CT] Asp700I 596 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 434 GTGCAC G[AT]GC[AT]^C AvaI 937 CTCGGG C^[CT]CG[GA]G AvaII 494 GGTCC G^G[AT]CC AviII 154 TGCGCA TGC^GCA BalI 112 TGGCCA TGG^CCA BcnI 1089 CCCGG CC^[GC]GG BfaI 372 CTAG C^TAG BglI 399 GCCGACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 515 AGATCT A^GATCT BlpI 750 GCTGAGC GC^T[ACGT]AGC BmyI 434 GTGCAC G[AGT]GC[ACT]^C Bpu1102I 750 GCTGAGC GC^T[ACGT]AGC BsgI 147 GTGCAG GTGCAG(16/14) BsiHKAI 434 GTGCAC G[AT]GC[AT]^C BsmAI 935 GTCTC GTCTC(1/5) BsoBI 937 CTCGGG C^[CT]CG[GA]G Bsp1286I 434 GTGCAC G[AGT]GC[ACT]^C BspLU11I 1146 ACATGT A^CATGT BspMI 614 GCAGGT ACCTGC(4/8) BsrI 880 ACTGG ACTGG(1/-1) BstUI 1061 CGCG CG^CG BstYI 515 AGATCT [GA]^GATC[CT] CelII 750 GCTGAGC GC^T[ACGT]AGC DraII 847 AGGGCCT [GA]G^G[ACGT]CC[CT] EaeI 112 TGGCCA [CT]^GGCC[GA] EarI 135 GAAGAG CTCTTC(1/4) EcoNI 590 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 847 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoRI 813 GAATTC G^AATTC FspI 154 TGCGCA TGC^GCA HapII 1090 CCGG C^CGG HgaI 1001 GCGTC GACGC(5/10) HpaII 1090 CCGG C^CGG Ksp632I 135 GAAGAG CTCTTC(1/4) MaeI 372 CTAG C^TAG MflI 515 AGATCT [GA]^GATC[CT] MluNI 112 TGGCCA TGG^CCA MscI 112 TGGCCA TGG^CCA MslI 276 CATCAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 1090 CCGG C^CGG MvnI 1061 CGCG CG^CG NciI 1089 CCCGG CC^[GC]GG PflMI 293 CCACATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SfaNI 396 GATGC GCATC(5/9) SpeI 371 ACTAGT A^CTAGT Van91I 293 CCACATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 515 AGATCT [GA]^GATC[CT] XmnI 596 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AfaI 177 GTAC GT^AC 944 GTAC BclI 121 TGATCA T^GATCA 535 TGATCA BsaJI 391 CCCTGG C^C[ACGT][ACGT]GG 727 CCCAGG BseRI 138 GAGGAG GAGGAG(10/8) 579 GAGGAG BsmFI 389 GTCCC GGGAC(10/14) 620 GTCCC BsrBI 855 CCGCTC CCGCTC(-3/-3) 213 GAGCGG BstF5I 281 GGATG GGATG(2/0) 395 GGATG CfoI 155 GCGC GCG^C 1060 GCGC FauI 854 CCCGC CCCGC(4/6) 215 GCGGG FbaI 121 TGATCA T^GATCA 535 TGATCA FokI 281 GGATG GGATG(9/13) 395 GGATG HhaI 155 GCGC GCG^C 1060 GCGC HinP1I 155 GCGC G^CGC 1060 GCGC HinfI 316 GATTC G^A[ACGT]TC 419 GATTC HphI 797 GGTGA GGTGA(8/7) 124 TCACC MseI 505 TTAA T^TAA 1075 TTAA NspI 1015 ACATGC [GA]CATG^[CT] 1146 ACATGT PstI 827 CTGCAG CTGCA^G 991 CTGCAG RsaI 177 GTAC GT^AC 944 GTAC SfcI 827 CTGCAG C^T[GA][CT]AG 991 CTGCAG TfiI 316 GATTC G^A[AT]TC 419 GATTC Tru9I 505 TTAA T^TAA 1075 TTAA Tsp45I 9 GTGAC ^GT[GC]AC 360 GTGAC Tsp509I 544 AATT ^AATT 814 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AciI AluI BbvI BpmI BsiYI BslI BssKI BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII ItaI MaeIII MboI MboII MnlI MspA1I MvaI MwoI NdeII NlaIII NlaIV PvuII Sau3AI Sau96I ScrFI TaqI TseI TspRI TthHB8I