################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI Alw44I ApaLI ApoI AscI AseI AsnI Asp718I AspI AviII AvrII BamHI BanI BbeI BciVI BclI BfrI BglII BlnI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmI BspDI BspEI BspHI BspLU11I BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI EagI EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I Van91I XbaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 846 GACGTC GACGT^C AccII 642 CGCG CG^CG AhdI 1177 GACACAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 603 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 1248 AGCGCT AGC^GCT AspEI 1177 GACACAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 76 GAGCTC G[AT]GC[AT]^C BalI 422 TGGCCA TGG^CCA BbrPI 568 CACGTG CAC^GTG BbvCI 1102 GCTGAGG CCTCAGC(-5/-2) BpmI 70 CTGGAG CTGGAG(16/14) BsaBI 690 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiHKAI 76 GAGCTC G[AT]GC[AT]^C Bsp1407I 1052 TGTACA T^GTACA BsrBI 1382 GAGCGG CCGCTC(-3/-3) BsrDI 468 CATTGC GCAATG(2/0) BsrFI 922 ACCGGC [GA]^CCGG[CT] BsrGI 1052 TGTACA T^GTACA BstAPI 1260 GCAGCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 642 CGCG CG^CG BstXI 300 CCAGCACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 63 GGATCT [GA]^GATC[CT] Cfr10I 922 ACCGGC [GA]^CCGG[CT] DrdI 1138 GACTGGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1172 CCGTGG C^C[GA][CT]GG Eam1105I 1177 GACACAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1248 AGCGCT AGC^GCT EcoNI 954 CCTTGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeII 1248 AGCGCT [GA]GCGC^[CT] MamI 690 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 63 GGATCT [GA]^GATC[CT] MluNI 422 TGGCCA TGG^CCA MscI 422 TGGCCA TGG^CCA MseI 770 TTAA T^TAA MvnI 642 CGCG CG^CG NheI 1236 GCTAGC G^CTAGC PmaCI 568 CACGTG CAC^GTG PmlI 568 CACGTG CAC^GTG PpuMI 1198 GGGACCC [GA]G^G[AT]CC[CT] SacI 76 GAGCTC GAGCT^C SfiI 90 GGCCTCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 59 CCCGGG CCC^GGG SspBI 1052 TGTACA T^GTACA StuI 623 AGGCCT AGG^CCT Tru9I 770 TTAA T^TAA Tsp509I 768 AATT ^AATT XcmI 446 CCAGTGCCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 63 GGATCT [GA]^GATC[CT] XmaCI 59 CCCGGG C^CCGGG XmaI 59 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AlwI 63 GGATC GGATC(4/5) 1041 GGATC ApaI 1071 GGGCCC GGGCC^C 1350 GGGCCC Asp700I 156 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1246 GAAGCGCTTC BcgI 135 CGACCTGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 177 GCACCTGCCTCG BlpI 396 GCTGAGC GC^T[ACGT]AGC 823 GCTGAGC Bpu1102I 396 GCTGAGC GC^T[ACGT]AGC 823 GCTGAGC BsaAI 568 CACGTG [CT]AC^GT[GA] 841 TACGTG BspMI 179 ACCTGC ACCTGC(4/8) 1003 ACCTGC CelII 396 GCTGAGC GC^T[ACGT]AGC 823 GCTGAGC CfoI 926 GCGC GCG^C 1249 GCGC DraIII 382 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG 1338 CACCTTGTG EaeI 131 TGGCCG [CT]^GGCC[GA] 422 TGGCCA HhaI 926 GCGC GCG^C 1249 GCGC HinP1I 926 GCGC G^CGC 1249 GCGC MaeIII 844 GTGAC ^GT[ACGT]AC 1216 GTCAC MslI 304 CACATTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 442 CACCCCAGTG MspA1I 267 CAGCTG C[AC]G^C[GT]G 1261 CAGCTG PaeR7I 185 CTCGAG C^TCGAG 547 CTCGAG PvuII 267 CAGCTG CAG^CTG 1261 CAGCTG SapI 561 GAAGAGC GCTCTTC(1/4) 913 GAAGAGC SmlI 185 CTCGAG C^T[CT][GA]AG 547 CTCGAG Tsp45I 844 GTGAC ^GT[GC]AC 1216 GTCAC XhoI 185 CTCGAG C^TCGAG 547 CTCGAG XmnI 156 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1246 GAAGCGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AciI AcyI AfaI AluI AocI AvaI AvaII BanII BbsI BbvI BcnI BfaI BglI BmyI BpuAI BsaHI BsaJI BseRI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI Bsu36I Cac8I Cfr13I DdeI DpnII DraII EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI Hin1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI TseI TspRI TthHB8I