##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI Alw44I ApaLI
ApoI AscI AseI AsnI Asp718I
AspI AviII AvrII BamHI BanI
BbeI BciVI BclI BfrI BglII
BlnI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmAI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI EagI
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
KspI MfeI MluI MroI MunI
NaeI NarI NcoI NdeI NgoMIV
NotI NruI NsiI NspI NspV
PacI PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I Van91I XbaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 846 GACGTC GACGT^C
AccII 642 CGCG CG^CG
AhdI 1177 GACACAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 603 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1248 AGCGCT AGC^GCT
AspEI 1177 GACACAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 76 GAGCTC G[AT]GC[AT]^C
BalI 422 TGGCCA TGG^CCA
BbrPI 568 CACGTG CAC^GTG
BbvCI 1102 GCTGAGG CCTCAGC(-5/-2)
BpmI 70 CTGGAG CTGGAG(16/14)
BsaBI 690 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 76 GAGCTC G[AT]GC[AT]^C
Bsp1407I 1052 TGTACA T^GTACA
BsrBI 1382 GAGCGG CCGCTC(-3/-3)
BsrDI 468 CATTGC GCAATG(2/0)
BsrFI 922 ACCGGC [GA]^CCGG[CT]
BsrGI 1052 TGTACA T^GTACA
BstAPI 1260 GCAGCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 642 CGCG CG^CG
BstXI 300 CCAGCACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 63 GGATCT [GA]^GATC[CT]
Cfr10I 922 ACCGGC [GA]^CCGG[CT]
DrdI 1138 GACTGGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1172 CCGTGG C^C[GA][CT]GG
Eam1105I 1177 GACACAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1248 AGCGCT AGC^GCT
EcoNI 954 CCTTGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 1248 AGCGCT [GA]GCGC^[CT]
MamI 690 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 63 GGATCT [GA]^GATC[CT]
MluNI 422 TGGCCA TGG^CCA
MscI 422 TGGCCA TGG^CCA
MseI 770 TTAA T^TAA
MvnI 642 CGCG CG^CG
NheI 1236 GCTAGC G^CTAGC
PmaCI 568 CACGTG CAC^GTG
PmlI 568 CACGTG CAC^GTG
PpuMI 1198 GGGACCC [GA]G^G[AT]CC[CT]
SacI 76 GAGCTC GAGCT^C
SfiI 90 GGCCTCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 59 CCCGGG CCC^GGG
SspBI 1052 TGTACA T^GTACA
StuI 623 AGGCCT AGG^CCT
Tru9I 770 TTAA T^TAA
Tsp509I 768 AATT ^AATT
XcmI 446 CCAGTGCCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 63 GGATCT [GA]^GATC[CT]
XmaCI 59 CCCGGG C^CCGGG
XmaI 59 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwI 63 GGATC GGATC(4/5)
1041 GGATC
ApaI 1071 GGGCCC GGGCC^C
1350 GGGCCC
Asp700I 156 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1246 GAAGCGCTTC
BcgI 135 CGACCTGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
177 GCACCTGCCTCG
BlpI 396 GCTGAGC GC^T[ACGT]AGC
823 GCTGAGC
Bpu1102I 396 GCTGAGC GC^T[ACGT]AGC
823 GCTGAGC
BsaAI 568 CACGTG [CT]AC^GT[GA]
841 TACGTG
BspMI 179 ACCTGC ACCTGC(4/8)
1003 ACCTGC
CelII 396 GCTGAGC GC^T[ACGT]AGC
823 GCTGAGC
CfoI 926 GCGC GCG^C
1249 GCGC
DraIII 382 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
1338 CACCTTGTG
EaeI 131 TGGCCG [CT]^GGCC[GA]
422 TGGCCA
HhaI 926 GCGC GCG^C
1249 GCGC
HinP1I 926 GCGC G^CGC
1249 GCGC
MaeIII 844 GTGAC ^GT[ACGT]AC
1216 GTCAC
MslI 304 CACATTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
442 CACCCCAGTG
MspA1I 267 CAGCTG C[AC]G^C[GT]G
1261 CAGCTG
PaeR7I 185 CTCGAG C^TCGAG
547 CTCGAG
PvuII 267 CAGCTG CAG^CTG
1261 CAGCTG
SapI 561 GAAGAGC GCTCTTC(1/4)
913 GAAGAGC
SmlI 185 CTCGAG C^T[CT][GA]AG
547 CTCGAG
Tsp45I 844 GTGAC ^GT[GC]AC
1216 GTCAC
XhoI 185 CTCGAG C^TCGAG
547 CTCGAG
XmnI 156 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1246 GAAGCGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcyI AfaI AluI AocI
AvaI AvaII BanII BbsI BbvI
BcnI BfaI BglI BmyI BpuAI
BsaHI BsaJI BseRI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI Bsu36I Cac8I Cfr13I
DdeI DpnII DraII EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI Hin1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MboI MboII MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI TseI TspRI
TthHB8I