##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AcsI AflII AflIII AhdI
AocI Aor51HI ApaI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbrPI BbsI BcgI BciVI BfrI
BglI BlnI BpmI BpuAI BsaAI
BsaBI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstZ17I
Bsu36I ClaI CpoI DraI DraII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoRI
EcoT22I FauI FseI FspI HgaI
HpaI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NcoI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PmaCI PmeI
PmlI PpuMI PshBI PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI Tth111I
Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AclI 381 AACGTT AA^CGTT
AcyI 946 GGCGCC G[GA]^CG[CT]C
AgeI 788 ACCGGT A^CCGGT
Alw44I 672 GTGCAC G^TGCAC
ApaLI 672 GTGCAC G^TGCAC
Asp700I 897 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 857 GGACC G^G[AT]CC
BbeI 946 GGCGCC GGCGC^C
BbvCI 30 CCTCAGC CCTCAGC(-5/-2)
BglII 995 AGATCT A^GATCT
BlpI 1053 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1053 GCTCAGC GC^T[ACGT]AGC
BsaHI 946 GGCGCC G[GA]^CG[CT]C
BsaWI 788 ACCGGT [AT]^CCGG[AT]
BsgI 364 GTGCAG GTGCAG(16/14)
Bsp1407I 298 TGTACA T^GTACA
BsrFI 788 ACCGGT [GA]^CCGG[CT]
BsrGI 298 TGTACA T^GTACA
BstEII 136 GGTCACC G^GT[ACGT]ACC
BstPI 136 GGTCACC G^GT[ACGT]ACC
BstXI 728 CCAACATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1053 GCTCAGC GC^T[ACGT]AGC
Cfr10I 788 ACCGGT [GA]^CCGG[CT]
DraIII 273 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 680 CCACGG C^C[GA][CT]GG
EcoNI 330 CCTGTTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 136 GGTCACC G^GT[ACGT]ACC
EcoRV 871 GATATC GAT^ATC
EcoT14I 98 CCAAGG C^C[AT][AT]GG
Hin1I 946 GGCGCC G[GA]^CG[CT]C
KasI 946 GGCGCC G^GCGCC
MspA1I 713 CAGCTG C[AC]G^C[GT]G
NarI 946 GGCGCC GG^CGCC
NdeI 977 CATATG CA^TATG
PinAI 788 ACCGGT A^CCGGT
PshAI 216 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 381 AACGTT AA^CGTT
PstI 808 CTGCAG CTGCA^G
PvuII 713 CAGCTG CAG^CTG
SacI 564 GAGCTC GAGCT^C
ScaI 1024 AGTACT AGT^ACT
SfoI 946 GGCGCC GGC^GCC
SmlI 526 CTCAAG C^T[CT][GA]AG
SspBI 298 TGTACA T^GTACA
SspI 402 AATATT AAT^ATT
StyI 98 CCAAGG C^C[AT][AT]GG
XbaI 1237 TCTAGA T^CTAGA
XcmI 725 CCACCAACATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 897 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwI 1272 GATCC GGATC(4/5)
234 GGATC
AspHI 564 GAGCTC G[AT]GC[AT]^C
672 GTGCAC
BanI 946 GGCGCC G^G[CT][GA]CC
1102 GGCACC
BclI 587 TGATCA T^GATCA
1252 TGATCA
BcnI 437 CCCGG CC^[GC]GG
801 CCCGG
BsaI 214 GAGACC GGTCTC(1/5)
125 GGTCTC
BsiHKAI 564 GAGCTC G[AT]GC[AT]^C
672 GTGCAC
BspHI 464 TCATGA T^CATGA
488 TCATGA
Cac8I 191 GCTTGC GC[ACGT]^[ACGT]GC
1035 GCACGC
FbaI 587 TGATCA T^GATCA
1252 TGATCA
HincII 375 GTCAAC GT[CT]^[GA]AC
631 GTCAAC
HindII 375 GTCAAC GT[CT]^[GA]AC
631 GTCAAC
HindIII 690 AAGCTT A^AGCTT
766 AAGCTT
MaeII 382 ACGT A^CGT
1009 ACGT
MseI 385 TTAA T^TAA
398 TTAA
MslI 139 CACCGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
465 CATGACTATG
NciI 437 CCCGG CC^[GC]GG
801 CCCGG
NspI 35 GCATGT [GA]CATG^[CT]
350 GCATGT
RcaI 464 TCATGA T^CATGA
488 TCATGA
SfcI 808 CTGCAG C^T[GA][CT]AG
909 CTACAG
TaqI 2 TCGA T^CGA
20 TCGA
TfiI 10 GATTC G^A[AT]TC
603 GATTC
Tru9I 385 TTAA T^TAA
398 TTAA
Tsp509I 387 AATT ^AATT
457 AATT
TthHB8I 2 TCGA T^CGA
20 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AfaI AluI AlwNI BanII BbvI
BfaI BmyI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI CfoI
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI TseI Tsp45I TspRI XhoII